Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Li, S.; Friesen, J.A.; Fei, H.; Ding, X.; Borst, D.W.
    The lobster mandibular organ produces soluble and membrane-bound forms of 3-hydroxy-3-methylglutaryl-CoA reductase (2004), Biochem. J., 381, 831-840.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.34 isozyme HMGR1, DNA and amino acid sequence determination and analysis, functional expression in Spodoptera frugiperda Sf9 cells via baculovirus infection system as His-tagged enzyme Homarus americanus
1.1.1.34 isozyme HMGR2, DNA and amino acid sequence determination and analysis Homarus americanus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.34 lovastatin
-
 Homarus americanus
1.1.1.34 mevalonic acid
-
 Homarus americanus
1.1.1.34 additional information no inhibition by farnesoate and farnesoic acid Homarus americanus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.34 membrane bound, isozyme HMGR2, about 25% of total enzyme content Homarus americanus 16020
-
1.1.1.34 soluble isozyme HMGR1, about 75% of total enzyme content Homarus americanus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.34 72000
-
-
 Homarus americanus
1.1.1.34 140000
-
 isozyme HMGR1, minor peak, gel filtration Homarus americanus
1.1.1.34 540000
-
 isozyme HMGR1, major peak, gel filtration Homarus americanus

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.34 Homarus americanus Q7YT62 lobster, 2 enzyme forms: a soluble and a membrane-bound enzyme
-
1.1.1.34 Homarus americanus Q7YT63 lobster, 2 enzyme forms: a soluble isozyme HMGR1 and a membrane-bound isozyme HMGR2
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.1.1.34 additional information the isozyme is not regulated by phosphorylation Homarus americanus
1.1.1.34 additional information the isozyme is probably not regulated by phosphorylation Homarus americanus

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.34 native soluble isozyme HMGR1 from mandibular organ by ultracentrifugation, anion exchange chromatography, ultrafiltration and gel filtration, recombinant enzyme from Sf9 insect cells by nickel affinity chromatography Homarus americanus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.34 mandibular organ
-
 Homarus americanus
-
1.1.1.34 additional information very low content in other tissues, higher enzyme activity in male organisms than in female, tissue distribution overview Homarus americanus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.34 0.0006
-
 about, native cells Homarus americanus
1.1.1.34 60
-
 recombinant Sf9 cells Homarus americanus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.34 (S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH + 2 H+
-
 Homarus americanus (R)-mevalonate + CoA + 2 NADP+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.34 oligomer 1 x 69000, recombinant His-tagged isozyme HMGR1, SDS-PAGE Homarus americanus

Synonyms

EC Number Synonyms Comment Organism
1.1.1.34 3-hydroxy-3-methylglutaryl CoA reductase
-
 Homarus americanus
1.1.1.34 HMGR
-
 Homarus americanus
1.1.1.34 HMGR1
-
 Homarus americanus
1.1.1.34 HMGR2
-
 Homarus americanus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.34 7.4
-
 assay at Homarus americanus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.1.34 0.00000045
-
 lovastatin pH 7.4, recombinant isozyme HMGR1 Homarus americanus
1.1.1.34 0.081
-
 mevalonic acid pH 7.4, recombinant isozyme HMGR1 Homarus americanus