Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Parry, N.J.; Beever, D.E.; Owen, E.; Vandenberghe, I.; Van Beeumen, J.; Bhat, M.K.
    Biochemical characterization and mechanism of action of a thermostable beta-glucosidase purified from Thermoascus aurantiacus (2001), Biochem. J., 353, 117-127.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.21 ethanol 30%, 40% activation Thermoascus aurantiacus
3.2.1.21 methanol 30%, 30% activation Thermoascus aurantiacus
3.2.1.21 propan-2-ol concentrations below 50%, activation Thermoascus aurantiacus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.21 beta-D-glucose
-
 Thermoascus aurantiacus
3.2.1.21 D-delta-gluconolactone
-
 Thermoascus aurantiacus
3.2.1.21 ethanol inhibition above 50% concentration Thermoascus aurantiacus
3.2.1.21 methanol inhibition above 50% concentration Thermoascus aurantiacus
3.2.1.21 propan-2-ol inhibition above 50% concentration, 50% inhibition at 90%-100% propan-2-ol Thermoascus aurantiacus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.21 120000
-
 3 * 120000, SDS-PAGE Thermoascus aurantiacus
3.2.1.21 350000
-
 gel filtration Thermoascus aurantiacus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.21 Thermoascus aurantiacus
-
 IMI216529
-
3.2.1.21 Thermoascus aurantiacus IMI216529
-
 IMI216529
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.21 DEAE-Sepharose, Ultrogel AcA 44, Mono P Thermoascus aurantiacus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.21 190000
-
 at pH 4.5 and 60°C Thermoascus aurantiacus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.21 4-methylumbelliferyl-beta-D-cellobiose + H2O
-
 Thermoascus aurantiacus 4-methylumbelliferyl-beta-D-glucopyranoside + beta-D-glucose
-
 ?
3.2.1.21 4-methylumbelliferyl-beta-D-cellobiose + H2O
-
 Thermoascus aurantiacus IMI216529 4-methylumbelliferyl-beta-D-glucopyranoside + beta-D-glucose
-
 ?
3.2.1.21 4-methylumbelliferyl-beta-D-glucopyranoside + H2O
-
 Thermoascus aurantiacus 4-methylumbelliferol + beta-D-glucose
-
 ?
3.2.1.21 arbutin + H2O
-
 Thermoascus aurantiacus beta-D-glucose + ?
-
 ?
3.2.1.21 beta-beta-trehalose + H2O
-
 Thermoascus aurantiacus beta-D-glucose
-
 ?
3.2.1.21 cellobiose + H2O
-
 Thermoascus aurantiacus 2 beta-D-glucose
-
 ?
3.2.1.21 cellobiose + H2O
-
 Thermoascus aurantiacus IMI216529 2 beta-D-glucose
-
 ?
3.2.1.21 cellobiose + methanol 25% methanol, transglycosylation activity Thermoascus aurantiacus methyl-beta-D-glucopyranoside + beta-D-glucose
-
 ?
3.2.1.21 CM-cellulose + H2O
-
 Thermoascus aurantiacus CM-cellulose + beta-D-glucose
-
 ?
3.2.1.21 esculin + H2O
-
 Thermoascus aurantiacus beta-D-glucose + ?
-
 ?
3.2.1.21 gentiobiose + H2O
-
 Thermoascus aurantiacus 2 beta-D-glucose
-
 ?
3.2.1.21 gentiobiose + H2O
-
 Thermoascus aurantiacus IMI216529 2 beta-D-glucose
-
 ?
3.2.1.21 laminarin + H2O 100times lower activity than with cellobiose Thermoascus aurantiacus laminarioligosaccharides + D-glucose
-
 ?
3.2.1.21 methyl-beta-D-glucopyranoside + H2O
-
 Thermoascus aurantiacus methanol + beta-D-glucopyranose
-
 ?
3.2.1.21 n-octyl-beta-D-glucopyranoside + H2O
-
 Thermoascus aurantiacus n-octanol + beta-D-glucose
-
 ?
3.2.1.21 o-nitrophenyl-beta-D-glucopyranoside + H2O
-
 Thermoascus aurantiacus o-nitrophenol + beta-D-glucose
-
 ?
3.2.1.21 p-nitrophenyl-beta-D-glucopyranoside + H2O
-
 Thermoascus aurantiacus p-nitrophenol + beta-D-glucose
-
 ?
3.2.1.21 salicin + H2O
-
 Thermoascus aurantiacus beta-D-glucose + ?
-
 ?
3.2.1.21 salicin + H2O
-
 Thermoascus aurantiacus IMI216529 beta-D-glucose + ?
-
 ?
3.2.1.21 sophorose + H2O
-
 Thermoascus aurantiacus 2 beta-D-glucose
-
 ?
3.2.1.21 sophorose + H2O
-
 Thermoascus aurantiacus IMI216529 2 beta-D-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.21 trimer 3 * 120000, SDS-PAGE Thermoascus aurantiacus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.21 80
-
-
 Thermoascus aurantiacus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.21 40 90 70% of maximal activity at 90°C, low activity below 60°C Thermoascus aurantiacus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.21 50
-
 no loss of activity after 48 h at pH 6.8 Thermoascus aurantiacus
3.2.1.21 70
-
 no loss of activity after 30 min at pH 5.2 Thermoascus aurantiacus
3.2.1.21 80
-
 90% loss of activity after 30 min at pH 5.2 Thermoascus aurantiacus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.21 136.4
-
 sophorose 60°C, pH 4.5 Thermoascus aurantiacus
3.2.1.21 140.7
-
 gentiobiose 60°C, pH 4.5 Thermoascus aurantiacus
3.2.1.21 234.8
-
 n-octyl-beta-D-glucopyranoside 60°C, pH 4.5 Thermoascus aurantiacus
3.2.1.21 235.2
-
 o-nitrophenyl-beta-D-glucopyranoside 60°C, pH 4.5 Thermoascus aurantiacus
3.2.1.21 242.2
-
 p-nitrophenyl-beta-D-glucopyranoside 60°C, pH 4.5 Thermoascus aurantiacus
3.2.1.21 284.2
-
 cellobiose 60°C, pH 4.5 Thermoascus aurantiacus
3.2.1.21 284.2
-
 beta,beta-trehalose 60°C, pH 4.5 Thermoascus aurantiacus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.21 4.5
-
-
 Thermoascus aurantiacus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.21 2.8 6.8 approx. 30% of maximal activity at pH 3.5 and pH 6.8, respectively Thermoascus aurantiacus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.21 0.0083
-
 D-delta-gluconolactone 60°C, pH 4.5 Thermoascus aurantiacus
3.2.1.21 0.29
-
 beta-D-glucose 60°C, pH 4.5 Thermoascus aurantiacus