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Literature summary extracted from

  • Lim, W.J.; Park, S.R.; Cho, S.J.; Kim, M.K.; Ryu, S.K.; Hong, S.Y.; Seo, W.T.; Kim, H.; Yun, H.D.
    Cloning and characterization of an intracellular isoamylase gene from Pectobacterium chrysanthemi PY35 (2001), Biochem. Biophys. Res. Commun., 287, 348-354.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.68
-
 Dickeya chrysanthemi

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.68 ammonium sulfate 0.5 M, 30% residual activity Dickeya chrysanthemi
3.2.1.68 CuCl2 5 mM, 30% residual activity Dickeya chrysanthemi
3.2.1.68 EDTA 5 mM, 80% residual activity Dickeya chrysanthemi
3.2.1.68 MgCl2 5 mM, 70% residual activity Dickeya chrysanthemi
3.2.1.68 MnCl2 5 mM, 80% residual activity Dickeya chrysanthemi
3.2.1.68 sodium dodecylsulfate 1 mM, no residual activity Dickeya chrysanthemi
3.2.1.68 ZnCl2 5 mM, 20% residual activity Dickeya chrysanthemi

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.68 CaCl2 5 mM, 120% of activity Dickeya chrysanthemi

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.68 74000
-
 x * 74100, deduced from gene sequence, x * 74000, SDS-PAGE Dickeya chrysanthemi
3.2.1.68 74100
-
 x * 74100, deduced from gene sequence, x * 74000, SDS-PAGE Dickeya chrysanthemi

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.68 Dickeya chrysanthemi
-
 PY35
-
3.2.1.68 Dickeya chrysanthemi PY35
-
 PY35
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.68 amylopectin + H2O
-
 Dickeya chrysanthemi ? hydrolysis of alpha-1,6-linkages ?
3.2.1.68 amylopectin + H2O
-
 Dickeya chrysanthemi PY35 ? hydrolysis of alpha-1,6-linkages ?
3.2.1.68 glycogen + H2O 80% activity compared to amylopectin Dickeya chrysanthemi ?
-
 ?
3.2.1.68 glycogen + H2O 80% activity compared to amylopectin Dickeya chrysanthemi PY35 ?
-
 ?
3.2.1.68 additional information no substrate: dextran, amylose Dickeya chrysanthemi ?
-
 ?
3.2.1.68 additional information no substrate: dextran, amylose Dickeya chrysanthemi PY35 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.68 ? x * 74100, deduced from gene sequence, x * 74000, SDS-PAGE Dickeya chrysanthemi

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.68 40
-
-
 Dickeya chrysanthemi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.68 7
-
-
 Dickeya chrysanthemi