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Literature summary extracted from

  • Punyasiri, P.A.; Abeysinghe, I.S.; Kumar, V.; Treutter, D.; Duy, D.; Gosch, C.; Martens, S.; Forkmann, G.; Fischer, T.C.
    Flavonoid biosynthesis in the tea plant Camellia sinensis: properties of enzymes of the prominent epicatechin and catechin pathways (2004), Arch. Biochem. Biophys., 431, 22-30.
    View publication on PubMed

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.17.1.3 additional information Camellia sinensis enzyme is important in biosynthesis of catchin, epigallocatechin, and anthocyanidins, flavonoid metabolism in tea leaves, overview ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.77 Camellia sinensis
-
-
-
1.17.1.3 Camellia sinensis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.17.1.3 partially Camellia sinensis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.1.77 leaf
-
 Camellia sinensis
-
1.17.1.3 leaf 2 young leaves and bud Camellia sinensis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.17.1.3 additional information
-
 activity of flavonoid metabolism enzymes in tea leaves Camellia sinensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.77 cyanidin + NADPH
-
 Camellia sinensis epicatechin + NADP+
-
 ?
1.17.1.3 (2R,3R)-dihydromyricetin + NADPH combined dihydroflavonol 4-reductase/leucoanthocyanidin 4-reductae activity Camellia sinensis gallocatechin + NADP+ + H2O
-
 ?
1.17.1.3 (2R,3R)-dihydroquercetin + NADPH combined dihydroflavonol 4-reductase/leucoanthocyanidin 4-reductae activity, 2-step reaction Camellia sinensis (2R,3S)-catechin + NADP+ + H2O
-
 ?
1.17.1.3 (2S)-eriodictyol + NADPH flavan formation by combined flavanone 4-reductase/leucoanthocyanidin 4-reductae activity Camellia sinensis luteoliflavan + NADP+ + H2O
-
 ?
1.17.1.3 (2S)-naringenin + NADPH flavan formation by combined flavanone 4-reductase/leucoanthocyanidin 4-reductae activity Camellia sinensis ?
-
 ?
1.17.1.3 2,3-trans-3,4-cis-leucoanthocyanidin + NADPH
-
 Camellia sinensis (2R,3S)-catechin + NADP+ + H2O
-
 ?
1.17.1.3 additional information enzyme is important in biosynthesis of catchin, epigallocatechin, and anthocyanidins, flavonoid metabolism in tea leaves, overview Camellia sinensis ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.1.77 ANR
-
 Camellia sinensis
1.17.1.3 LAR
-
 Camellia sinensis
1.17.1.3 leucoanthocyanidin 4-reductase
-
 Camellia sinensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.17.1.3 25
-
 assay at Camellia sinensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.17.1.3 6
-
 assay at, combined flavanone 4-reductase/leucoanthocyanidin 4-reductae activity Camellia sinensis
1.17.1.3 7.6
-
 combined dihydroflavonol 4-reductase/leucoanthocyanidin 4-reductae activity Camellia sinensis

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.77 NADPH
-
 Camellia sinensis
1.17.1.3 NADPH dependent on Camellia sinensis