BRENDA - Enzyme Database show

Synthesis of prostaglandin F ethanolamide by prostaglandin F synthase and identification of Bimatoprost as a potent inhibitor of the enzyme: new enzyme assay method using LC/ESI/MS

Koda, N.; Tsutsui, Y.; Niwa, H.; Ito, S.; Woodward, D.F.; Watanabe, K.; Arch. Biochem. Biophys. 424, 128-136 (2004)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.1.1.188
expressed in Escherichia coli HB101 cells; expression Escherichia coli HB101
Homo sapiens
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.188
(5Z)-7-[(1R,2R,3R,5S)-3,5-dihydroxy-2-[(1E,3S)-3-hydroxy-5-phenylpent-1-en-1-yl]cyclopentyl]-N-ethylhept-5-enamide
bimatoprost
Homo sapiens
1.1.1.188
(5Z)-7-[(1R,4S,5S,6R)-6-[(1E)-oct-1-en-1-yl]-2-azabicyclo[2.2.1]hept-5-yl]hept-5-enoic acid
ONO1370
Homo sapiens
1.1.1.188
(5Z)-7-[(1R,4S,5S,6R)-6-[(1E,3S)-3-hydroxyoct-1-en-1-yl]-2-azabicyclo[2.2.1]hept-5-yl]hept-5-enoic acid
ONO1349
Homo sapiens
1.1.1.188
(5Z)-7-[(1S,4S,5S,6R)-2-methyl-6-[(1E)-oct-1-en-1-yl]-2-azabicyclo[2.2.1]hept-5-yl]hept-5-enoic acid
ONO1373
Homo sapiens
1.1.1.188
(Z)-7-[(1R,4S,5S,6R)-((E)-6-oct-1-enyl)-2-aza-bicyclo[2.2.1]hept-5-yl]-hept-5-enoic acid
inhibition of PGH2 9,11-endoperoxide reductase activity, slight inhibition of prostamide D2 11-ketoreductase and PGD2 11-ketoreductase activity
Homo sapiens
1.1.1.188
(Z)-7-[(1R,4S,5S,6R)-2-methyl-6-((E)-oct-1-enyl)-2-aza-bicyclo[2.2.1]hept-5-yl]-hept-5-enoic acid
inhibition of PGH2 9,11-endoperoxide reductase activity, slight inhibition of prostamide D2 11-ketoreductase and PGD2 11-ketoreductase activity, competitive, binds to the active site
Homo sapiens
1.1.1.188
(Z)-7-[(1R,4S,5S,6R)-6-((E)-(S)-3-hydroxy-oct-1-enyl)-2-aza-bicyclo[2.2.1]hept-5-yl]-hept-5-enoic acid
inhibition of PGH2 9,11-endoperoxide reductase activity, slight inhibition of prostamide D2 11-ketoreductase and PGD2 11-ketoreductase activity
Homo sapiens
1.1.1.188
bimatoprost
ethyl amide analogue of 17-phenyl-trinor PGF2alpha, inhibits all enzyme activities noncompetitively at low concentration, binds to a site different from the active site; potent inhibitor
Homo sapiens
1.1.1.188
ONO1349
-
Homo sapiens
1.1.1.188
ONO1370
-
Homo sapiens
1.1.1.188
ONO1373
-
Homo sapiens
1.1.1.188
Tolrestat
; inhibition of all 3 enzyme activities, most effective inhibiting the PGD2 11-ketoreductase activity
Homo sapiens
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.188
Homo sapiens
-
-
-
1.1.1.188
Homo sapiens
P42330
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.1.1.188
; recombinant enzyme from Escherichia coli, to homogeneity
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.188
additional information
substrate and product detection by liquid chromatographic-electrospray ionization-mass spectrometry
654368
Homo sapiens
?
-
-
-
-
1.1.1.188
prostaglandin D2 + NADPH
-
654368
Homo sapiens
prostaglandin F2alpha + NADP+
-
-
-
?
1.1.1.188
prostaglandin D2 + NADPH + H+
-
654368
Homo sapiens
prostaglandin 9alpha,11beta-F2 + NADP+
-
-
-
?
1.1.1.188
prostaglandin D2 + NADPH + H+
-
654368
Homo sapiens
9alpha,11beta-prostaglandin F2 + NADP+
-
-
-
?
1.1.1.188
prostaglandin D2 ethanolamide + NADPH
i.e. prostamide D2
654368
Homo sapiens
prostaglandin 9alpha,11beta-F2 ethanolamide + NADP+
i.e. 91lpha,11beta-prostamide F2
-
-
?
1.1.1.188
prostaglandin H2 + NADPH
-
654368
Homo sapiens
prostaglandin F2alpha + NADP+
-
-
-
?
1.1.1.188
prostaglandin H2 + NADPH
-
654368
Homo sapiens
prostaglandin 9alpha,11beta-F2 + NADP+
-
-
-
?
1.1.1.188
prostaglandin H2 + NADPH + H+
-
654368
Homo sapiens
prostaglandin F2alpha + NADP+
-
-
-
?
1.1.1.188
prostamide D2 + NADPH + H+
-
654368
Homo sapiens
9alpha,11beta-prostamide F2 + NADP+
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.1.1.188
37
-
PGD2 11-ketoreductase activity assay at
Homo sapiens
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.188
6.5
-
PGD2 11-ketoreductase activity assay at
Homo sapiens
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.188
NADPH
;
Homo sapiens
IC50 Value
EC Number
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
1.1.1.188
0.005
-
recombinant enzyme, using prostaglandin D2 as substrate, in 0.1 M potassium phosphate buffer, pH 6.5, at 37°C
Homo sapiens
(5Z)-7-[(1R,2R,3R,5S)-3,5-dihydroxy-2-[(1E,3S)-3-hydroxy-5-phenylpent-1-en-1-yl]cyclopentyl]-N-ethylhept-5-enamide
1.1.1.188
0.005
-
recombinant enzyme, using prostaglandin D2 as substrate,in 0.1 M potassium phosphate buffer, pH 6.5, at 37°C
Homo sapiens
bimatoprost
1.1.1.188
0.006
-
recombinant enzyme, using prostaglandin H2 as substrate, in 0.1 M potassium phosphate buffer, pH 6.5, at 37°C
Homo sapiens
(5Z)-7-[(1R,2R,3R,5S)-3,5-dihydroxy-2-[(1E,3S)-3-hydroxy-5-phenylpent-1-en-1-yl]cyclopentyl]-N-ethylhept-5-enamide
1.1.1.188
0.006
-
recombinant enzyme, using prostaglandin H2 as substrate,in 0.1 M potassium phosphate buffer, pH 6.5, at 37°C
Homo sapiens
bimatoprost
1.1.1.188
0.06
-
recombinant enzyme, using prostamide D2 as substrate, in 0.1 M potassium phosphate buffer, pH 6.5, at 37°C
Homo sapiens
(5Z)-7-[(1R,2R,3R,5S)-3,5-dihydroxy-2-[(1E,3S)-3-hydroxy-5-phenylpent-1-en-1-yl]cyclopentyl]-N-ethylhept-5-enamide
1.1.1.188
0.06
-
recombinant enzyme, using prostaglandin H2 as substrate, in 0.1 M potassium phosphate buffer, pH 6.5, at 37°C
Homo sapiens
(5Z)-7-[(1S,4S,5S,6R)-2-methyl-6-[(1E)-oct-1-en-1-yl]-2-azabicyclo[2.2.1]hept-5-yl]hept-5-enoic acid
1.1.1.188
0.06
-
recombinant enzyme, using prostamide D2 as substrate,in 0.1 M potassium phosphate buffer, pH 6.5, at 37°C
Homo sapiens
bimatoprost
1.1.1.188
0.06
-
recombinant enzyme, using prostaglandin H2 2 as substrate,in 0.1 M potassium phosphate buffer, pH 6.5, at 37°C
Homo sapiens
ONO1373
1.1.1.188
0.1
-
recombinant enzyme, using prostaglandin D2 as substrate,in 0.1 M potassium phosphate buffer, pH 6.5, at 37°C
Homo sapiens
Tolrestat
1.1.1.188
0.13
-
recombinant enzyme, using prostaglandin H2 as substrate, in 0.1 M potassium phosphate buffer, pH 6.5, at 37°C
Homo sapiens
(5Z)-7-[(1R,4S,5S,6R)-6-[(1E,3S)-3-hydroxyoct-1-en-1-yl]-2-azabicyclo[2.2.1]hept-5-yl]hept-5-enoic acid
1.1.1.188
0.13
-
recombinant enzyme, using prostaglandin H2 as substrate,in 0.1 M potassium phosphate buffer, pH 6.5, at 37°C
Homo sapiens
ONO1349
1.1.1.188
0.3
-
recombinant enzyme, using prostaglandin H2 as substrate, in 0.1 M potassium phosphate buffer, pH 6.5, at 37°C
Homo sapiens
(5Z)-7-[(1R,4S,5S,6R)-6-[(1E)-oct-1-en-1-yl]-2-azabicyclo[2.2.1]hept-5-yl]hept-5-enoic acid
1.1.1.188
0.3
-
recombinant enzyme, using prostaglandin H2 as substrate,in 0.1 M potassium phosphate buffer, pH 6.5, at 37°C
Homo sapiens
ONO1370
1.1.1.188
0.3
-
recombinant enzyme, using prostaglandin H2 as substrate,in 0.1 M potassium phosphate buffer, pH 6.5, at 37°C
Homo sapiens
Tolrestat
1.1.1.188
0.45
-
recombinant enzyme, using prostamide D2 as substrate,in 0.1 M potassium phosphate buffer, pH 6.5, at 37°C
Homo sapiens
Tolrestat
1.1.1.188
1
-
IC50 about 1 mM, recombinant enzyme, using prostaglandin D2 as substrate, in 0.1 M potassium phosphate buffer, pH 6.5, at 37°C; IC50 about 1 mM, recombinant enzyme, using prostamide D2 as substrate, in 0.1 M potassium phosphate buffer, pH 6.5, at 37°C
Homo sapiens
(5Z)-7-[(1R,4S,5S,6R)-6-[(1E,3S)-3-hydroxyoct-1-en-1-yl]-2-azabicyclo[2.2.1]hept-5-yl]hept-5-enoic acid
1.1.1.188
1
-
IC50 about 1 mM, recombinant enzyme, using prostaglandin D2 as substrate, in 0.1 M potassium phosphate buffer, pH 6.5, at 37°C; IC50 about 1 mM, recombinant enzyme, using prostamide D2 as substrate, in 0.1 M potassium phosphate buffer, pH 6.5, at 37°C
Homo sapiens
(5Z)-7-[(1R,4S,5S,6R)-6-[(1E)-oct-1-en-1-yl]-2-azabicyclo[2.2.1]hept-5-yl]hept-5-enoic acid
1.1.1.188
1
-
IC50 about 1 mM, recombinant enzyme, using prostaglandin D2 as substrate, in 0.1 M potassium phosphate buffer, pH 6.5, at 37°C; IC50 about 1 mM, recombinant enzyme, using prostamide D2 as substrate, in 0.1 M potassium phosphate buffer, pH 6.5, at 37°C
Homo sapiens
(5Z)-7-[(1S,4S,5S,6R)-2-methyl-6-[(1E)-oct-1-en-1-yl]-2-azabicyclo[2.2.1]hept-5-yl]hept-5-enoic acid
1.1.1.188
1
-
recombinant enzyme, using prostaglandin D2 as substrate,in 0.1 M potassium phosphate buffer, pH 6.5, at 37°C; recombinant enzyme, using prostamide D2 as substrate,in 0.1 M potassium phosphate buffer, pH 6.5, at 37°C
Homo sapiens
ONO1349
1.1.1.188
1
-
recombinant enzyme, using prostaglandin D2 as substrate,in 0.1 M potassium phosphate buffer, pH 6.5, at 37°C; recombinant enzyme, using prostamide D2 as substrate,in 0.1 M potassium phosphate buffer, pH 6.5, at 37°C
Homo sapiens
ONO1370
1.1.1.188
1
-
recombinant enzyme, using prostaglandin D2 as substrate,in 0.1 M potassium phosphate buffer, pH 6.5, at 37°C; recombinant enzyme, using prostamide D2 2 as substrate,in 0.1 M potassium phosphate buffer, pH 6.5, at 37°C
Homo sapiens
ONO1373
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.188
expressed in Escherichia coli HB101 cells
Homo sapiens
1.1.1.188
expression Escherichia coli HB101
Homo sapiens
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.188
NADPH
-
Homo sapiens
IC50 Value (protein specific)
EC Number
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
1.1.1.188
0.005
-
recombinant enzyme, using prostaglandin D2 as substrate, in 0.1 M potassium phosphate buffer, pH 6.5, at 37°C
Homo sapiens
(5Z)-7-[(1R,2R,3R,5S)-3,5-dihydroxy-2-[(1E,3S)-3-hydroxy-5-phenylpent-1-en-1-yl]cyclopentyl]-N-ethylhept-5-enamide
1.1.1.188
0.005
-
recombinant enzyme, using prostaglandin D2 as substrate,in 0.1 M potassium phosphate buffer, pH 6.5, at 37°C
Homo sapiens
bimatoprost
1.1.1.188
0.006
-
recombinant enzyme, using prostaglandin H2 as substrate, in 0.1 M potassium phosphate buffer, pH 6.5, at 37°C
Homo sapiens
(5Z)-7-[(1R,2R,3R,5S)-3,5-dihydroxy-2-[(1E,3S)-3-hydroxy-5-phenylpent-1-en-1-yl]cyclopentyl]-N-ethylhept-5-enamide
1.1.1.188
0.006
-
recombinant enzyme, using prostaglandin H2 as substrate,in 0.1 M potassium phosphate buffer, pH 6.5, at 37°C
Homo sapiens
bimatoprost
1.1.1.188
0.06
-
recombinant enzyme, using prostamide D2 as substrate, in 0.1 M potassium phosphate buffer, pH 6.5, at 37°C
Homo sapiens
(5Z)-7-[(1R,2R,3R,5S)-3,5-dihydroxy-2-[(1E,3S)-3-hydroxy-5-phenylpent-1-en-1-yl]cyclopentyl]-N-ethylhept-5-enamide
1.1.1.188
0.06
-
recombinant enzyme, using prostaglandin H2 as substrate, in 0.1 M potassium phosphate buffer, pH 6.5, at 37°C
Homo sapiens
(5Z)-7-[(1S,4S,5S,6R)-2-methyl-6-[(1E)-oct-1-en-1-yl]-2-azabicyclo[2.2.1]hept-5-yl]hept-5-enoic acid
1.1.1.188
0.06
-
recombinant enzyme, using prostamide D2 as substrate,in 0.1 M potassium phosphate buffer, pH 6.5, at 37°C
Homo sapiens
bimatoprost
1.1.1.188
0.06
-
recombinant enzyme, using prostaglandin H2 2 as substrate,in 0.1 M potassium phosphate buffer, pH 6.5, at 37°C
Homo sapiens
ONO1373
1.1.1.188
0.1
-
recombinant enzyme, using prostaglandin D2 as substrate,in 0.1 M potassium phosphate buffer, pH 6.5, at 37°C
Homo sapiens
Tolrestat
1.1.1.188
0.13
-
recombinant enzyme, using prostaglandin H2 as substrate, in 0.1 M potassium phosphate buffer, pH 6.5, at 37°C
Homo sapiens
(5Z)-7-[(1R,4S,5S,6R)-6-[(1E,3S)-3-hydroxyoct-1-en-1-yl]-2-azabicyclo[2.2.1]hept-5-yl]hept-5-enoic acid
1.1.1.188
0.13
-
recombinant enzyme, using prostaglandin H2 as substrate,in 0.1 M potassium phosphate buffer, pH 6.5, at 37°C
Homo sapiens
ONO1349
1.1.1.188
0.3
-
recombinant enzyme, using prostaglandin H2 as substrate, in 0.1 M potassium phosphate buffer, pH 6.5, at 37°C
Homo sapiens
(5Z)-7-[(1R,4S,5S,6R)-6-[(1E)-oct-1-en-1-yl]-2-azabicyclo[2.2.1]hept-5-yl]hept-5-enoic acid
1.1.1.188
0.3
-
recombinant enzyme, using prostaglandin H2 as substrate,in 0.1 M potassium phosphate buffer, pH 6.5, at 37°C
Homo sapiens
ONO1370
1.1.1.188
0.3
-
recombinant enzyme, using prostaglandin H2 as substrate,in 0.1 M potassium phosphate buffer, pH 6.5, at 37°C
Homo sapiens
Tolrestat
1.1.1.188
0.45
-
recombinant enzyme, using prostamide D2 as substrate,in 0.1 M potassium phosphate buffer, pH 6.5, at 37°C
Homo sapiens
Tolrestat
1.1.1.188
1
-
IC50 about 1 mM, recombinant enzyme, using prostaglandin D2 as substrate, in 0.1 M potassium phosphate buffer, pH 6.5, at 37°C; IC50 about 1 mM, recombinant enzyme, using prostamide D2 as substrate, in 0.1 M potassium phosphate buffer, pH 6.5, at 37°C
Homo sapiens
(5Z)-7-[(1R,4S,5S,6R)-6-[(1E,3S)-3-hydroxyoct-1-en-1-yl]-2-azabicyclo[2.2.1]hept-5-yl]hept-5-enoic acid
1.1.1.188
1
-
IC50 about 1 mM, recombinant enzyme, using prostaglandin D2 as substrate, in 0.1 M potassium phosphate buffer, pH 6.5, at 37°C; IC50 about 1 mM, recombinant enzyme, using prostamide D2 as substrate, in 0.1 M potassium phosphate buffer, pH 6.5, at 37°C
Homo sapiens
(5Z)-7-[(1R,4S,5S,6R)-6-[(1E)-oct-1-en-1-yl]-2-azabicyclo[2.2.1]hept-5-yl]hept-5-enoic acid
1.1.1.188
1
-
IC50 about 1 mM, recombinant enzyme, using prostaglandin D2 as substrate, in 0.1 M potassium phosphate buffer, pH 6.5, at 37°C; IC50 about 1 mM, recombinant enzyme, using prostamide D2 as substrate, in 0.1 M potassium phosphate buffer, pH 6.5, at 37°C
Homo sapiens
(5Z)-7-[(1S,4S,5S,6R)-2-methyl-6-[(1E)-oct-1-en-1-yl]-2-azabicyclo[2.2.1]hept-5-yl]hept-5-enoic acid
1.1.1.188
1
-
recombinant enzyme, using prostaglandin D2 as substrate,in 0.1 M potassium phosphate buffer, pH 6.5, at 37°C; recombinant enzyme, using prostamide D2 as substrate,in 0.1 M potassium phosphate buffer, pH 6.5, at 37°C
Homo sapiens
ONO1349
1.1.1.188
1
-
recombinant enzyme, using prostaglandin D2 as substrate,in 0.1 M potassium phosphate buffer, pH 6.5, at 37°C; recombinant enzyme, using prostamide D2 as substrate,in 0.1 M potassium phosphate buffer, pH 6.5, at 37°C
Homo sapiens
ONO1370
1.1.1.188
1
-
recombinant enzyme, using prostaglandin D2 as substrate,in 0.1 M potassium phosphate buffer, pH 6.5, at 37°C; recombinant enzyme, using prostamide D2 2 as substrate,in 0.1 M potassium phosphate buffer, pH 6.5, at 37°C
Homo sapiens
ONO1373
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.188
(5Z)-7-[(1R,2R,3R,5S)-3,5-dihydroxy-2-[(1E,3S)-3-hydroxy-5-phenylpent-1-en-1-yl]cyclopentyl]-N-ethylhept-5-enamide
bimatoprost
Homo sapiens
1.1.1.188
(5Z)-7-[(1R,4S,5S,6R)-6-[(1E)-oct-1-en-1-yl]-2-azabicyclo[2.2.1]hept-5-yl]hept-5-enoic acid
ONO1370
Homo sapiens
1.1.1.188
(5Z)-7-[(1R,4S,5S,6R)-6-[(1E,3S)-3-hydroxyoct-1-en-1-yl]-2-azabicyclo[2.2.1]hept-5-yl]hept-5-enoic acid
ONO1349
Homo sapiens
1.1.1.188
(5Z)-7-[(1S,4S,5S,6R)-2-methyl-6-[(1E)-oct-1-en-1-yl]-2-azabicyclo[2.2.1]hept-5-yl]hept-5-enoic acid
ONO1373
Homo sapiens
1.1.1.188
(Z)-7-[(1R,4S,5S,6R)-((E)-6-oct-1-enyl)-2-aza-bicyclo[2.2.1]hept-5-yl]-hept-5-enoic acid
inhibition of PGH2 9,11-endoperoxide reductase activity, slight inhibition of prostamide D2 11-ketoreductase and PGD2 11-ketoreductase activity
Homo sapiens
1.1.1.188
(Z)-7-[(1R,4S,5S,6R)-2-methyl-6-((E)-oct-1-enyl)-2-aza-bicyclo[2.2.1]hept-5-yl]-hept-5-enoic acid
inhibition of PGH2 9,11-endoperoxide reductase activity, slight inhibition of prostamide D2 11-ketoreductase and PGD2 11-ketoreductase activity, competitive, binds to the active site
Homo sapiens
1.1.1.188
(Z)-7-[(1R,4S,5S,6R)-6-((E)-(S)-3-hydroxy-oct-1-enyl)-2-aza-bicyclo[2.2.1]hept-5-yl]-hept-5-enoic acid
inhibition of PGH2 9,11-endoperoxide reductase activity, slight inhibition of prostamide D2 11-ketoreductase and PGD2 11-ketoreductase activity
Homo sapiens
1.1.1.188
bimatoprost
ethyl amide analogue of 17-phenyl-trinor PGF2alpha, inhibits all enzyme activities noncompetitively at low concentration, binds to a site different from the active site
Homo sapiens
1.1.1.188
bimatoprost
potent inhibitor
Homo sapiens
1.1.1.188
ONO1349
-
Homo sapiens
1.1.1.188
ONO1370
-
Homo sapiens
1.1.1.188
ONO1373
-
Homo sapiens
1.1.1.188
Tolrestat
inhibition of all 3 enzyme activities, most effective inhibiting the PGD2 11-ketoreductase activity
Homo sapiens
1.1.1.188
Tolrestat
-
Homo sapiens
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.188
-
Homo sapiens
1.1.1.188
recombinant enzyme from Escherichia coli, to homogeneity
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.188
additional information
substrate and product detection by liquid chromatographic-electrospray ionization-mass spectrometry
654368
Homo sapiens
?
-
-
-
-
1.1.1.188
prostaglandin D2 + NADPH
-
654368
Homo sapiens
prostaglandin F2alpha + NADP+
-
-
-
?
1.1.1.188
prostaglandin D2 + NADPH + H+
-
654368
Homo sapiens
prostaglandin 9alpha,11beta-F2 + NADP+
-
-
-
?
1.1.1.188
prostaglandin D2 + NADPH + H+
-
654368
Homo sapiens
9alpha,11beta-prostaglandin F2 + NADP+
-
-
-
?
1.1.1.188
prostaglandin D2 ethanolamide + NADPH
i.e. prostamide D2
654368
Homo sapiens
prostaglandin 9alpha,11beta-F2 ethanolamide + NADP+
i.e. 91lpha,11beta-prostamide F2
-
-
?
1.1.1.188
prostaglandin H2 + NADPH
-
654368
Homo sapiens
prostaglandin F2alpha + NADP+
-
-
-
?
1.1.1.188
prostaglandin H2 + NADPH
-
654368
Homo sapiens
prostaglandin 9alpha,11beta-F2 + NADP+
-
-
-
?
1.1.1.188
prostaglandin H2 + NADPH + H+
-
654368
Homo sapiens
prostaglandin F2alpha + NADP+
-
-
-
?
1.1.1.188
prostamide D2 + NADPH + H+
-
654368
Homo sapiens
9alpha,11beta-prostamide F2 + NADP+
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.1.1.188
37
-
PGD2 11-ketoreductase activity assay at
Homo sapiens
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.188
6.5
-
PGD2 11-ketoreductase activity assay at
Homo sapiens