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Literature summary extracted from

  • Hashimoto, W.; Miyake, O.; Nankai, H.; Murata, K.
    Molecular identification of an alpha-L-rhamnosidase from Bacillus sp strain GL1 as an enzyme involved in complete metabolism of gellan (2003), Arch. Biochem. Biophys., 415, 235-244.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.40
-
 Bacillus sp. (in: Bacteria)

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.40 CuCl2 1 mM, 4% residual activity; 1 mM, 70% residual activity Bacillus sp. (in: Bacteria)
3.2.1.40 D-glucose 10 mM, 56% residual activity Bacillus sp. (in: Bacteria)
3.2.1.40 FeCl3 1 mM, 76% residual activity Bacillus sp. (in: Bacteria)
3.2.1.40 HgCl2 0.1 mM, 0.23% residual activity Bacillus sp. (in: Bacteria)
3.2.1.40 L-rhamnose 10 mM, 14% residual activity; 10 mM, 37% residual activity Bacillus sp. (in: Bacteria)
3.2.1.40 p-chloromercuribenzoic acid 0.1 mM, 50% residual activity Bacillus sp. (in: Bacteria)
3.2.1.40 ZnCl2 1 mM, 73% residual activity Bacillus sp. (in: Bacteria)

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.40 0.119
-
 p-nitrophenyl-alpha-L-rhamnopyranoside pH 7.0 Bacillus sp. (in: Bacteria)
3.2.1.40 0.282
-
 p-nitrophenyl-alpha-L-rhamnopyranoside pH 7.0 Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.40 98000
-
 5 * 98300, deduced from gene sequence, 5 * 98000, SDS-PAGE Bacillus sp. (in: Bacteria)
3.2.1.40 98300
-
 5 * 98300, deduced from gene sequence, 5 * 98000, SDS-PAGE Bacillus sp. (in: Bacteria)
3.2.1.40 100000
-
 gel filtration, PAGE Bacillus sp. (in: Bacteria)
3.2.1.40 105000
-
 1 * 106000, deduced from gene sequence, 1 * 105000, SDS-PAGE Bacillus sp. (in: Bacteria)
3.2.1.40 106000
-
 1 * 106000, deduced from gene sequence, 1 * 105000, SDS-PAGE Bacillus sp. (in: Bacteria)
3.2.1.40 500000
-
 gel filtration, PAGE Bacillus sp. (in: Bacteria)

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.40 additional information Bacillus sp. (in: Bacteria) isozyme plays a crucial role in complete metabolism of gellan ?
-
 ?
3.2.1.40 additional information Bacillus sp. (in: Bacteria) GL1 isozyme plays a crucial role in complete metabolism of gellan ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.40 Bacillus sp. (in: Bacteria) Q93RE7 ene rhaB, when grown on gellan medium, only this isoform is expressed
-
3.2.1.40 Bacillus sp. (in: Bacteria) Q93RE8 gene rhaA
-
3.2.1.40 Bacillus sp. (in: Bacteria) GL1 Q93RE7 ene rhaB, when grown on gellan medium, only this isoform is expressed
-
3.2.1.40 Bacillus sp. (in: Bacteria) GL1 Q93RE8 gene rhaA
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.40 proteolytic modification
-
 Bacillus sp. (in: Bacteria)

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.40
-
 Bacillus sp. (in: Bacteria)

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.40 54
-
 pH 7.0 Bacillus sp. (in: Bacteria)
3.2.1.40 54.4
-
 pH 7.0 Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.40 gellan + H2O
-
 Bacillus sp. (in: Bacteria) L-rhamnose + ?
-
 ?
3.2.1.40 gellan + H2O
-
 Bacillus sp. (in: Bacteria) GL1 L-rhamnose + ?
-
 ?
3.2.1.40 additional information isozyme plays a crucial role in complete metabolism of gellan Bacillus sp. (in: Bacteria) ?
-
 ?
3.2.1.40 additional information isozyme plays a crucial role in complete metabolism of gellan Bacillus sp. (in: Bacteria) GL1 ?
-
 ?
3.2.1.40 naringin + H2O
-
 Bacillus sp. (in: Bacteria) L-rhamnose + ?
-
 ?
3.2.1.40 naringin + H2O
-
 Bacillus sp. (in: Bacteria) GL1 L-rhamnose + ?
-
 ?
3.2.1.40 p-nitrophenyl-alpha-L-rhamnopyranoside + H2O
-
 Bacillus sp. (in: Bacteria) p-nitrophenol + alpha-L-rhamnopyranose
-
 ?
3.2.1.40 p-nitrophenyl-alpha-L-rhamnopyranoside + H2O
-
 Bacillus sp. (in: Bacteria) GL1 p-nitrophenol + alpha-L-rhamnopyranose
-
 ?
3.2.1.40 p-nitrophenyl-beta-D-fucopyranoside + H2O
-
 Bacillus sp. (in: Bacteria) p-nitrophenol + beta-D-fucopyranose 2.9% of activity compared to p-nitrophenyl-alpha-L-rhamnopyranoside ?
3.2.1.40 p-nitrophenyl-beta-D-fucopyranoside + H2O
-
 Bacillus sp. (in: Bacteria) GL1 p-nitrophenol + beta-D-fucopyranose 2.9% of activity compared to p-nitrophenyl-alpha-L-rhamnopyranoside ?
3.2.1.40 p-nitrophenyl-beta-D-galactopyranoside + H2O
-
 Bacillus sp. (in: Bacteria) p-nitrophenol + beta-D-galactopyranose 1.6% of activity compared to p-nitrophenyl-alpha-L-rhamnopyranoside ?

Subunits

EC Number Subunits Comment Organism
3.2.1.40 monomer 1 * 106000, deduced from gene sequence, 1 * 105000, SDS-PAGE Bacillus sp. (in: Bacteria)
3.2.1.40 pentamer 5 * 98300, deduced from gene sequence, 5 * 98000, SDS-PAGE Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.40 40
-
-
 Bacillus sp. (in: Bacteria)

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.40 6.5 7
-
 Bacillus sp. (in: Bacteria)