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Literature summary extracted from

  • Ludwig, R.; Salamon, A.; Varga, J.; Zamocky, M.; Peterbauer, C.K.; Kulbe, K.D.; Haltrich, D.
    Characterization of cellobiose dehydrogenase from the white rot fungi Trametes pubescens and Trametes villosa (2004), Appl. Microbiol. Biotechnol., 64, 213-222.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.99.18 0.00069
-
 cytochrome c pH 4.5, 30°C, reaction with lactose Trametes pubescens
1.1.99.18 0.00099
-
 ferricenium ion pH 4.5, 30°C, reaction with lactose Trametes pubescens
1.1.99.18 0.0012
-
 ferricenium ion pH 4.5, 30°C, reaction with lactose Trametes villosa
1.1.99.18 0.0018
-
 cytochrome c pH 4.5, 30°C, reaction with lactose Trametes villosa
1.1.99.18 0.0049
-
 2,6-dichlorophenol indophenol pH 4.5, 30°C, reaction with lactose Trametes pubescens
1.1.99.18 0.0055
-
 K3Fe(CN)6 pH 4.5, 30°C, reaction with lactose Trametes villosa
1.1.99.18 0.0086
-
 K3Fe(CN)6 pH 4.5, 30°C, reaction with lactose Trametes pubescens
1.1.99.18 0.0099
-
 2,6-dichlorophenol indophenol pH 4.5, 30°C, reaction with lactose Trametes villosa
1.1.99.18 0.011
-
 1,4-benzoquinone pH 4.5, 30°C, reaction with lactose Trametes villosa
1.1.99.18 0.018
-
 3,5-di-tert-butyl-1,2-benzoquinone pH 4.5, 30°C, reaction with lactose Trametes pubescens
1.1.99.18 0.019
-
 1,4-benzoquinone pH 4.5, 30°C, reaction with lactose Trametes pubescens
1.1.99.18 0.041
-
 3,5-di-tert-butyl-1,2-benzoquinone pH 4.5, 30°C, reaction with lactose Trametes villosa
1.1.99.18 0.21
-
 cellobiose pH 4.5, 30°C, electron acceptor: 2,6-dichlorophenol indophenol Trametes villosa
1.1.99.18 0.21
-
 cellobiose pH 4.5, 30°C, electron acceptor: 2,6-dichlorophenol indophenol Trametes pubescens
1.1.99.18 0.42
-
 cellotetraose pH 4.5, 30°C, electron acceptor: 2,6-dichlorophenol indophenol Trametes pubescens
1.1.99.18 0.46
-
 cellopentaose pH 4.5, 30°C, electron acceptor: 2,6-dichlorophenol indophenol Trametes pubescens
1.1.99.18 0.47
-
 cellotetraose pH 4.5, 30°C, electron acceptor: 2,6-dichlorophenol indophenol Trametes villosa
1.1.99.18 0.51
-
 cellopentaose pH 4.5, 30°C, electron acceptor: 2,6-dichlorophenol indophenol Trametes villosa
1.1.99.18 1.8
-
 thiocellobiose pH 4.5, 30°C, electron acceptor: 2,6-dichlorophenol indophenol Trametes pubescens
1.1.99.18 2.4
-
 lactose pH 4.5, 30°C, electron acceptor: 2,6-dichlorophenol indophenol Trametes pubescens
1.1.99.18 2.5
-
 thiocellobiose pH 4.5, 30°C, electron acceptor: 2,6-dichlorophenol indophenol Trametes villosa
1.1.99.18 3.7
-
 lactose pH 4.5, 30°C, electron acceptor: 2,6-dichlorophenol indophenol Trametes villosa
1.1.99.18 7.3
-
 xylobiose pH 4.5, 30°C, electron acceptor: 2,6-dichlorophenol indophenol Trametes pubescens
1.1.99.18 7.7
-
 xylobiose pH 4.5, 30°C, electron acceptor: 2,6-dichlorophenol indophenol Trametes villosa
1.1.99.18 350
-
 maltose pH 4.5, 30°C, electron acceptor: 2,6-dichlorophenol indophenol Trametes villosa
1.1.99.18 390
-
 maltose pH 4.5, 30°C, electron acceptor: 2,6-dichlorophenol indophenol Trametes pubescens
1.1.99.18 890
-
 D-glucose pH 4.5, 30°C, electron acceptor: 2,6-dichlorophenol indophenol Trametes pubescens
1.1.99.18 1300
-
 D-glucose pH 4.5, 30°C, electron acceptor: 2,6-dichlorophenol indophenol Trametes villosa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.99.18 extracellular
-
 Trametes villosa
-
-
1.1.99.18 extracellular
-
 Trametes pubescens
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.99.18 89000
-
 gel filtration Trametes villosa
1.1.99.18 90000
-
 gel filtration Trametes pubescens
1.1.99.18 98000
-
 1 * 98000, SDS-PAGE Trametes villosa
1.1.99.18 100000
-
 1 * 100000, SDS-PAGE Trametes pubescens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.99.18 additional information Trametes versicolor proposed role of the enzyme in the early events of lignocellulose degradation and wood colonisation ?
-
 ?
1.1.99.18 additional information Trametes hirsuta proposed role of the enzyme in the early events of lignocellulose degradation and wood colonisation ?
-
 ?
1.1.99.18 additional information Trametes ochracea proposed role of the enzyme in the early events of lignocellulose degradation and wood colonisation ?
-
 ?
1.1.99.18 additional information Trametes villosa proposed role of the enzyme in the early events of lignocellulose degradation and wood colonisation ?
-
 ?
1.1.99.18 additional information Trametes pubescens proposed role of the enzyme in the early events of lignocellulose degradation and wood colonisation ?
-
 ?
1.1.99.18 additional information Trametes maxima proposed role of the enzyme in the early events of lignocellulose degradation and wood colonisation ?
-
 ?
1.1.99.18 additional information Trametes gibbosa proposed role of the enzyme in the early events of lignocellulose degradation and wood colonisation ?
-
 ?
1.1.99.18 additional information Trametes cotonea proposed role of the enzyme in the early events of lignocellulose degradation and wood colonisation ?
-
 ?
1.1.99.18 additional information Trametes incerta proposed role of the enzyme in the early events of lignocellulose degradation and wood colonisation ?
-
 ?
1.1.99.18 additional information Trametes meyenii proposed role of the enzyme in the early events of lignocellulose degradation and wood colonisation ?
-
 ?
1.1.99.18 additional information Trametes suaveolens proposed role of the enzyme in the early events of lignocellulose degradation and wood colonisation ?
-
 ?
1.1.99.18 additional information Trametes villosa CBS 334.49 proposed role of the enzyme in the early events of lignocellulose degradation and wood colonisation ?
-
 ?
1.1.99.18 additional information Trametes suaveolens MB 51 proposed role of the enzyme in the early events of lignocellulose degradation and wood colonisation ?
-
 ?
1.1.99.18 additional information Trametes ochracea MB 49 proposed role of the enzyme in the early events of lignocellulose degradation and wood colonisation ?
-
 ?
1.1.99.18 additional information Trametes versicolor MB 52 proposed role of the enzyme in the early events of lignocellulose degradation and wood colonisation ?
-
 ?
1.1.99.18 additional information Trametes hirsuta MB 50 proposed role of the enzyme in the early events of lignocellulose degradation and wood colonisation ?
-
 ?
1.1.99.18 additional information Trametes maxima CBS 250.69 proposed role of the enzyme in the early events of lignocellulose degradation and wood colonisation ?
-
 ?
1.1.99.18 additional information Trametes gibbosa MB 187 proposed role of the enzyme in the early events of lignocellulose degradation and wood colonisation ?
-
 ?
1.1.99.18 additional information Trametes cotonea CBS 352.80 proposed role of the enzyme in the early events of lignocellulose degradation and wood colonisation ?
-
 ?
1.1.99.18 additional information Trametes incerta CBS 455.76 proposed role of the enzyme in the early events of lignocellulose degradation and wood colonisation ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.99.18 Trametes cotonea
-
 CBS 352.80
-
1.1.99.18 Trametes cotonea CBS 352.80
-
 CBS 352.80
-
1.1.99.18 Trametes gibbosa
-
 MB 187
-
1.1.99.18 Trametes gibbosa MB 187
-
 MB 187
-
1.1.99.18 Trametes hirsuta
-
 MB 50
-
1.1.99.18 Trametes hirsuta MB 50
-
 MB 50
-
1.1.99.18 Trametes incerta
-
 CBS 455.76
-
1.1.99.18 Trametes incerta CBS 455.76
-
 CBS 455.76
-
1.1.99.18 Trametes maxima
-
 CBS 250.69
-
1.1.99.18 Trametes maxima CBS 250.69
-
 CBS 250.69
-
1.1.99.18 Trametes meyenii
-
 CBS 453.76, weak activity
-
1.1.99.18 Trametes ochracea
-
 MB 49
-
1.1.99.18 Trametes ochracea MB 49
-
 MB 49
-
1.1.99.18 Trametes pubescens
-
-
-
1.1.99.18 Trametes suaveolens
-
 MB 51
-
1.1.99.18 Trametes suaveolens MB 51
-
 MB 51
-
1.1.99.18 Trametes versicolor
-
 MB 52
-
1.1.99.18 Trametes versicolor MB 52
-
 MB 52
-
1.1.99.18 Trametes villosa
-
 CBS 334.49
-
1.1.99.18 Trametes villosa CBS 334.49
-
 CBS 334.49
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.99.18
-
 Trametes villosa
1.1.99.18
-
 Trametes pubescens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.99.18 culture medium cellulose-containing Trametes villosa
-
1.1.99.18 culture medium cellulose-containing Trametes pubescens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.99.18 62
-
 assay detects both intact and truncated enzyme Trametes pubescens
1.1.99.18 69
-
 assay detects both intact and truncated enzyme Trametes villosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.99.18 cellobiose + 2,6-dichlorophenol indophenol
-
 Trametes villosa cellobiono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
1.1.99.18 cellobiose + 2,6-dichlorophenol indophenol
-
 Trametes pubescens cellobiono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
1.1.99.18 cellobiose + 2,6-dichlorophenol indophenol
-
 Trametes villosa CBS 334.49 cellobiono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
1.1.99.18 cellopentaose + 2,6-dichlorophenol indophenol
-
 Trametes villosa cellopentaono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
1.1.99.18 cellopentaose + 2,6-dichlorophenol indophenol
-
 Trametes pubescens cellopentaono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
1.1.99.18 cellopentaose + 2,6-dichlorophenol indophenol
-
 Trametes villosa CBS 334.49 cellopentaono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
1.1.99.18 cellotetraose + 2,6-dichlorophenol indophenol
-
 Trametes villosa cellotetrono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
1.1.99.18 cellotetraose + 2,6-dichlorophenol indophenol
-
 Trametes pubescens cellotetrono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
1.1.99.18 cellotetraose + 2,6-dichlorophenol indophenol
-
 Trametes villosa CBS 334.49 cellotetrono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
1.1.99.18 D-glucose + 2,6-dichlorophenol indophenol
-
 Trametes villosa D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
1.1.99.18 D-glucose + 2,6-dichlorophenol indophenol
-
 Trametes pubescens D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
1.1.99.18 D-glucose + 2,6-dichlorophenol indophenol
-
 Trametes villosa CBS 334.49 D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
1.1.99.18 lactose + 1,4-benzoquinone
-
 Trametes villosa 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + 1,4-benzoquinol
-
 ?
1.1.99.18 lactose + 1,4-benzoquinone
-
 Trametes pubescens 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + 1,4-benzoquinol
-
 ?
1.1.99.18 lactose + 2,6-dichlorophenol indophenol
-
 Trametes versicolor 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
1.1.99.18 lactose + 2,6-dichlorophenol indophenol
-
 Trametes hirsuta 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
1.1.99.18 lactose + 2,6-dichlorophenol indophenol
-
 Trametes ochracea 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
1.1.99.18 lactose + 2,6-dichlorophenol indophenol
-
 Trametes villosa 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
1.1.99.18 lactose + 2,6-dichlorophenol indophenol
-
 Trametes pubescens 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
1.1.99.18 lactose + 2,6-dichlorophenol indophenol
-
 Trametes maxima 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
1.1.99.18 lactose + 2,6-dichlorophenol indophenol
-
 Trametes gibbosa 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
1.1.99.18 lactose + 2,6-dichlorophenol indophenol
-
 Trametes cotonea 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
1.1.99.18 lactose + 2,6-dichlorophenol indophenol
-
 Trametes incerta 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
1.1.99.18 lactose + 2,6-dichlorophenol indophenol
-
 Trametes meyenii 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
1.1.99.18 lactose + 2,6-dichlorophenol indophenol
-
 Trametes suaveolens 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
1.1.99.18 lactose + 2,6-dichlorophenol indophenol
-
 Trametes suaveolens MB 51 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
1.1.99.18 lactose + 2,6-dichlorophenol indophenol
-
 Trametes ochracea MB 49 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
1.1.99.18 lactose + 2,6-dichlorophenol indophenol
-
 Trametes versicolor MB 52 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
1.1.99.18 lactose + 2,6-dichlorophenol indophenol
-
 Trametes hirsuta MB 50 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
1.1.99.18 lactose + 2,6-dichlorophenol indophenol
-
 Trametes maxima CBS 250.69 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
1.1.99.18 lactose + 2,6-dichlorophenol indophenol
-
 Trametes gibbosa MB 187 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
1.1.99.18 lactose + 2,6-dichlorophenol indophenol
-
 Trametes cotonea CBS 352.80 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
1.1.99.18 lactose + 2,6-dichlorophenol indophenol
-
 Trametes incerta CBS 455.76 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
1.1.99.18 lactose + 3,5-di-tert-butyl-1,2-benzoquinone
-
 Trametes villosa 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + ?
-
 ?
1.1.99.18 lactose + 3,5-di-tert-butyl-1,2-benzoquinone
-
 Trametes pubescens 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + ?
-
 ?
1.1.99.18 lactose + cytochrome c
-
 Trametes versicolor 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced cytochrome c
-
 ?
1.1.99.18 lactose + cytochrome c
-
 Trametes hirsuta 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced cytochrome c
-
 ?
1.1.99.18 lactose + cytochrome c
-
 Trametes ochracea 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced cytochrome c
-
 ?
1.1.99.18 lactose + cytochrome c
-
 Trametes villosa 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced cytochrome c
-
 ?
1.1.99.18 lactose + cytochrome c
-
 Trametes pubescens 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced cytochrome c
-
 ?
1.1.99.18 lactose + cytochrome c
-
 Trametes maxima 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced cytochrome c
-
 ?
1.1.99.18 lactose + cytochrome c
-
 Trametes gibbosa 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced cytochrome c
-
 ?
1.1.99.18 lactose + cytochrome c
-
 Trametes cotonea 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced cytochrome c
-
 ?
1.1.99.18 lactose + cytochrome c
-
 Trametes incerta 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced cytochrome c
-
 ?
1.1.99.18 lactose + cytochrome c
-
 Trametes meyenii 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced cytochrome c
-
 ?
1.1.99.18 lactose + cytochrome c
-
 Trametes suaveolens 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced cytochrome c
-
 ?
1.1.99.18 lactose + cytochrome c
-
 Trametes suaveolens MB 51 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced cytochrome c
-
 ?
1.1.99.18 lactose + cytochrome c
-
 Trametes ochracea MB 49 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced cytochrome c
-
 ?
1.1.99.18 lactose + cytochrome c
-
 Trametes versicolor MB 52 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced cytochrome c
-
 ?
1.1.99.18 lactose + cytochrome c
-
 Trametes hirsuta MB 50 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced cytochrome c
-
 ?
1.1.99.18 lactose + cytochrome c
-
 Trametes maxima CBS 250.69 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced cytochrome c
-
 ?
1.1.99.18 lactose + cytochrome c
-
 Trametes gibbosa MB 187 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced cytochrome c
-
 ?
1.1.99.18 lactose + cytochrome c
-
 Trametes cotonea CBS 352.80 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced cytochrome c
-
 ?
1.1.99.18 lactose + cytochrome c
-
 Trametes incerta CBS 455.76 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced cytochrome c
-
 ?
1.1.99.18 lactose + Fe(CN)63-
-
 Trametes villosa 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + Fe(CN)64-
-
 ?
1.1.99.18 lactose + Fe(CN)63-
-
 Trametes pubescens 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + Fe(CN)64-
-
 ?
1.1.99.18 lactose + ferricenium ion
-
 Trametes villosa 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + ?
-
 ?
1.1.99.18 lactose + ferricenium ion
-
 Trametes pubescens 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + ?
-
 ?
1.1.99.18 lactose + K3Fe(CN)6
-
 Trametes villosa 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced K3Fe(CN)6
-
 r
1.1.99.18 lactose + K3Fe(CN)6
-
 Trametes pubescens 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced K3Fe(CN)6
-
 r
1.1.99.18 additional information proposed role of the enzyme in the early events of lignocellulose degradation and wood colonisation Trametes versicolor ?
-
 ?
1.1.99.18 additional information proposed role of the enzyme in the early events of lignocellulose degradation and wood colonisation Trametes hirsuta ?
-
 ?
1.1.99.18 additional information proposed role of the enzyme in the early events of lignocellulose degradation and wood colonisation Trametes ochracea ?
-
 ?
1.1.99.18 additional information proposed role of the enzyme in the early events of lignocellulose degradation and wood colonisation Trametes villosa ?
-
 ?
1.1.99.18 additional information proposed role of the enzyme in the early events of lignocellulose degradation and wood colonisation Trametes pubescens ?
-
 ?
1.1.99.18 additional information proposed role of the enzyme in the early events of lignocellulose degradation and wood colonisation Trametes maxima ?
-
 ?
1.1.99.18 additional information proposed role of the enzyme in the early events of lignocellulose degradation and wood colonisation Trametes gibbosa ?
-
 ?
1.1.99.18 additional information proposed role of the enzyme in the early events of lignocellulose degradation and wood colonisation Trametes cotonea ?
-
 ?
1.1.99.18 additional information proposed role of the enzyme in the early events of lignocellulose degradation and wood colonisation Trametes incerta ?
-
 ?
1.1.99.18 additional information proposed role of the enzyme in the early events of lignocellulose degradation and wood colonisation Trametes meyenii ?
-
 ?
1.1.99.18 additional information proposed role of the enzyme in the early events of lignocellulose degradation and wood colonisation Trametes suaveolens ?
-
 ?
1.1.99.18 additional information proposed role of the enzyme in the early events of lignocellulose degradation and wood colonisation Trametes villosa CBS 334.49 ?
-
 ?
1.1.99.18 additional information proposed role of the enzyme in the early events of lignocellulose degradation and wood colonisation Trametes suaveolens MB 51 ?
-
 ?
1.1.99.18 additional information proposed role of the enzyme in the early events of lignocellulose degradation and wood colonisation Trametes ochracea MB 49 ?
-
 ?
1.1.99.18 additional information proposed role of the enzyme in the early events of lignocellulose degradation and wood colonisation Trametes versicolor MB 52 ?
-
 ?
1.1.99.18 additional information proposed role of the enzyme in the early events of lignocellulose degradation and wood colonisation Trametes hirsuta MB 50 ?
-
 ?
1.1.99.18 additional information proposed role of the enzyme in the early events of lignocellulose degradation and wood colonisation Trametes maxima CBS 250.69 ?
-
 ?
1.1.99.18 additional information proposed role of the enzyme in the early events of lignocellulose degradation and wood colonisation Trametes gibbosa MB 187 ?
-
 ?
1.1.99.18 additional information proposed role of the enzyme in the early events of lignocellulose degradation and wood colonisation Trametes cotonea CBS 352.80 ?
-
 ?
1.1.99.18 additional information proposed role of the enzyme in the early events of lignocellulose degradation and wood colonisation Trametes incerta CBS 455.76 ?
-
 ?
1.1.99.18 thiocellobiose + 2,6-dichlorophenol indophenol
-
 Trametes villosa thiocellobiono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
1.1.99.18 thiocellobiose + 2,6-dichlorophenol indophenol
-
 Trametes pubescens thiocellobiono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
1.1.99.18 xylobiose + 2,6-dichlorophenol indophenol
-
 Trametes villosa 4-O-beta-D-xylopyranosyl-D-xylono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
1.1.99.18 xylobiose + 2,6-dichlorophenol indophenol
-
 Trametes pubescens 4-O-beta-D-xylopyranosyl-D-xylono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.99.18 monomer 1 * 100000, SDS-PAGE Trametes pubescens
1.1.99.18 monomer 1 * 98000, SDS-PAGE Trametes villosa

Synonyms

EC Number Synonyms Comment Organism
1.1.99.18 Cdh
-
 Trametes versicolor
1.1.99.18 Cdh
-
 Trametes hirsuta
1.1.99.18 Cdh
-
 Trametes ochracea
1.1.99.18 Cdh
-
 Trametes maxima
1.1.99.18 Cdh
-
 Trametes gibbosa
1.1.99.18 Cdh
-
 Trametes cotonea
1.1.99.18 Cdh
-
 Trametes incerta
1.1.99.18 Cdh
-
 Trametes meyenii
1.1.99.18 Cdh
-
 Trametes suaveolens
1.1.99.18 TpCDH
-
 Trametes pubescens
1.1.99.18 TvCDH
-
 Trametes villosa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.99.18 24
-
 stable Trametes villosa
1.1.99.18 24
-
 6 h, 23% loss of activity Trametes pubescens
1.1.99.18 30
-
 half-life: 160 min Trametes villosa
1.1.99.18 30
-
 half-life: 34 min Trametes pubescens
1.1.99.18 45
-
 half-life: 17 min Trametes pubescens
1.1.99.18 45
-
 half-life: 97 min Trametes villosa
1.1.99.18 60
-
 half-life: 2.5 min Trametes villosa
1.1.99.18 60
-
 half-life: less than 1 min Trametes pubescens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.99.18 1.31
-
 D-glucose pH 4.5, 30°C, electron acceptor: 2,6-dichlorophenol indophenol Trametes pubescens
1.1.99.18 1.92
-
 maltose pH 4.5, 30°C, electron acceptor: 2,6-dichlorophenol indophenol Trametes pubescens
1.1.99.18 1.92
-
 glucose pH 4.5, 30°C, electron acceptor: 2,6-dichlorophenol indophenol Trametes villosa
1.1.99.18 2.08
-
 maltose pH 4.5, 30°C, electron acceptor: 2,6-dichlorophenol indophenol Trametes villosa
1.1.99.18 13.9
-
 cellopentaose pH 4.5, 30°C, electron acceptor: 2,6-dichlorophenol indophenol Trametes pubescens
1.1.99.18 14.2
-
 cellotetraose pH 4.5, 30°C, electron acceptor: 2,6-dichlorophenol indophenol Trametes villosa
1.1.99.18 14.7
-
 cellopentaose pH 4.5, 30°C, electron acceptor: 2,6-dichlorophenol indophenol Trametes villosa
1.1.99.18 15
-
 cellotetraose pH 4.5, 30°C, electron acceptor: 2,6-dichlorophenol indophenol Trametes pubescens
1.1.99.18 16.9
-
 3,5-di-tert-butyl-1,2-benzoquinone pH 4.5, 30°C, reaction with lactose Trametes villosa
1.1.99.18 17.2
-
 xylobiose pH 4.5, 30°C, electron acceptor: 2,6-dichlorophenol indophenol Trametes villosa
1.1.99.18 18.3
-
 xylobiose pH 4.5, 30°C, electron acceptor: 2,6-dichlorophenol indophenol Trametes pubescens
1.1.99.18 19.4
-
 thiocellobiose pH 4.5, 30°C, electron acceptor: 2,6-dichlorophenol indophenol Trametes pubescens
1.1.99.18 20.8
-
 1,4-benzoquinone pH 4.5, 30°C, reaction with lactose Trametes villosa
1.1.99.18 21.4
-
 thiocellobiose pH 4.5, 30°C, electron acceptor: 2,6-dichlorophenol indophenol Trametes villosa
1.1.99.18 21.9
-
 cellobiose pH 4.5, 30°C, electron acceptor: 2,6-dichlorophenol indophenol Trametes pubescens
1.1.99.18 23.6
-
 cellobiose pH 4.5, 30°C, electron acceptor: 2,6-dichlorophenol indophenol Trametes villosa
1.1.99.18 24.2
-
 3,5-di-tert-butyl-1,2-benzoquinone pH 4.5, 30°C, reaction with lactose Trametes pubescens
1.1.99.18 25.6
-
 2,6-dichlorophenol indophenol pH 4.5, 30°C, reaction with lactose Trametes pubescens
1.1.99.18 25.8
-
 lactose pH 4.5, 30°C, electron acceptor: 2,6-dichlorophenol indophenol Trametes pubescens
1.1.99.18 26.9
-
 lactose pH 4.5, 30°C, electron acceptor: 2,6-dichlorophenol indophenol Trametes villosa
1.1.99.18 27.5
-
 1,4-benzoquinone pH 4.5, 30°C, reaction with lactose Trametes pubescens
1.1.99.18 27.8
-
 K3Fe(CN)6 pH 4.5, 30°C, reaction with lactose Trametes pubescens
1.1.99.18 29.4
-
 cytochrome c pH 4.5, 30°C, reaction with lactose Trametes pubescens
1.1.99.18 32.5
-
 cytochrome c pH 4.5, 30°C, reaction with lactose Trametes villosa
1.1.99.18 34.7
-
 ferricenium ion pH 4.5, 30°C, reaction with lactose Trametes pubescens
1.1.99.18 35.6
-
 ferricenium ion pH 4.5, 30°C, reaction with lactose Trametes villosa
1.1.99.18 36.9
-
 K3Fe(CN)6 pH 4.5, 30°C, reaction with lactose Trametes villosa
1.1.99.18 38.1
-
 2,6-dichlorophenol indophenol pH 4.5, 30°C, reaction with lactose Trametes villosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.99.18 3.5
-
 lactose + cytochrome c, in acetate buffer Trametes pubescens
1.1.99.18 3.5 4.5 lactose + cytochrome c, in acetate buffer Trametes villosa
1.1.99.18 4.5
-
 lactose + 2,6-dichlorophenol indophenol or cytochrome c, in citrate buffer Trametes pubescens
1.1.99.18 4.5
-
 lactose + cytochrome c, in citrate buffer Trametes villosa
1.1.99.18 5
-
 lactose + 2,6-dichlorophenol indophenol, in citrate buffer Trametes villosa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.99.18 3 5 pH 3.0: about 60% of maximal activity, pH 5.0: about 70% of maximal activity, lactose + cytochrome c, in acetate buffer Trametes villosa
1.1.99.18 3 5 pH 3.0: about 70% of maximal activity, pH 5.0: about 40% of maximal activity, lactose + cytochrome c, in acetate buffer Trametes pubescens
1.1.99.18 3.5 5.5 pH 3.5: about 50% of maximal activity, pH 5.5: about 20% of maximal activity, lactose + 2,6-dichlorophenol indophenol, in citrate buffer Trametes pubescens
1.1.99.18 3.5 5.5 pH 3.5: about 50% of maximal activity, pH 5.5: about 20% of maximal activity, lactose + cytochrome c, in citrate buffer Trametes villosa
1.1.99.18 4 6 pH 4.0: about 70% of maximal activity, pH 6.0: about 40% of maximal activity, lactose + 2,6-dichlorophenol indophenol, in citrate buffer Trametes pubescens
1.1.99.18 4 6 pH 4.0: about 75% of maximal activity, pH 6.0: about 65% of maximal activity, lactose + 2,6-dichlorophenol indophenol, in citrate buffer Trametes villosa

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.99.18 flavin haemoflavoprotein Trametes versicolor
1.1.99.18 flavin haemoflavoprotein Trametes hirsuta
1.1.99.18 flavin haemoflavoprotein Trametes ochracea
1.1.99.18 flavin haemoflavoprotein Trametes villosa
1.1.99.18 flavin haemoflavoprotein Trametes pubescens
1.1.99.18 flavin haemoflavoprotein Trametes maxima
1.1.99.18 flavin haemoflavoprotein Trametes gibbosa
1.1.99.18 flavin haemoflavoprotein Trametes cotonea
1.1.99.18 flavin haemoflavoprotein Trametes incerta
1.1.99.18 flavin haemoflavoprotein Trametes meyenii
1.1.99.18 flavin haemoflavoprotein Trametes suaveolens
1.1.99.18 heme haemoflavoprotein Trametes versicolor
1.1.99.18 heme haemoflavoprotein Trametes hirsuta
1.1.99.18 heme haemoflavoprotein Trametes ochracea
1.1.99.18 heme haemoflavoprotein Trametes villosa
1.1.99.18 heme haemoflavoprotein Trametes pubescens
1.1.99.18 heme haemoflavoprotein Trametes maxima
1.1.99.18 heme haemoflavoprotein Trametes gibbosa
1.1.99.18 heme haemoflavoprotein Trametes cotonea
1.1.99.18 heme haemoflavoprotein Trametes incerta
1.1.99.18 heme haemoflavoprotein Trametes meyenii
1.1.99.18 heme haemoflavoprotein Trametes suaveolens

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.1.99.18 Trametes villosa isoelectric focusing
-
 4.2
1.1.99.18 Trametes pubescens isoelectric focusing
-
 4.2