Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • van den Broek, L.A.M.; Struijs, K.; Verdoes, J.C.; Beldman, G.; Voragen, A.G.J.
    Cloning and characterization of two alpha-glucosidases from Bifidobacterium adolescentis DSM20083 (2003), Appl. Microbiol. Biotechnol., 61, 55-60.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.10 construction of genetic library, DNA sequence determination and analysis of gene aglA, amino acid sequence determination, expression of the truncated enzyme fused to the N-terminus of lacZ in Escherichia coli XL1-Blue Bifidobacterium adolescentis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.10 1.05
-
 4-nitrophenyl alpha-D-glucopyranoside pH 6.5, 30°C Bifidobacterium adolescentis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.10 68000
-
 gel filtration Bifidobacterium adolescentis
3.2.1.10 71000
-
 1 * 71000, SDS-PAGE, 1 * 72138, recombinant fusion protein, amino acid sequence calculation Bifidobacterium adolescentis
3.2.1.10 72138
-
 1 * 71000, SDS-PAGE, 1 * 72138, recombinant fusion protein, amino acid sequence calculation Bifidobacterium adolescentis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.10 Bifidobacterium adolescentis Q9AF93 strain DSM 20083, gene aglA
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.10 recombinant enzyme from Escherichia coli Bifidobacterium adolescentis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.10 4-methylumbelliferyl alpha-D-glucoside + H2O
-
 Bifidobacterium adolescentis 4-methylumbelliferol + alpha-D-glucose
-
 ?
3.2.1.10 4-nitrophenyl alpha-D-glucopyranoside + H2O
-
 Bifidobacterium adolescentis 4-nitrophenol + alpha-D-glucopyranose
-
 ?
3.2.1.10 isomaltose + H2O
-
 Bifidobacterium adolescentis ?
-
 ?
3.2.1.10 isomaltriose + H2O
-
 Bifidobacterium adolescentis ?
-
 ?
3.2.1.10 additional information substrate specificity, enzyme is also capable to perform a transglycosylation producing oligosaccharides from trehalose and with low activity from sucrose, but no hydrolytic activity is detected with sucrose Bifidobacterium adolescentis ?
-
 ?
3.2.1.10 trehalose + H2O low activity Bifidobacterium adolescentis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.10 monomer 1 * 71000, SDS-PAGE, 1 * 72138, recombinant fusion protein, amino acid sequence calculation Bifidobacterium adolescentis

Synonyms

EC Number Synonyms Comment Organism
3.2.1.10 AglA
-
 Bifidobacterium adolescentis
3.2.1.10 More the enzyme belongs to family 13 of glycosyl gydrolases Bifidobacterium adolescentis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.10 37
-
-
 Bifidobacterium adolescentis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.10 6.6
-
-
 Bifidobacterium adolescentis