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Literature summary extracted from

  • Ludwig, R.; Haltrich, D.
    Optimisation of cellobiose dehydrogenase production by the fungus Sclerotium (Athelia) rolfsii (2003), Appl. Microbiol. Biotechnol., 61, 32-39.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.1.99.18 synthesis the enzyme contains a protease-sensitive linker region, can be cleaved by endogenous proteases into a catalytically active flavin fragment and an inactive haem domain. Cleavage can be prevented by using increased concentrations of peptone or certain amino acids such as Val or Leu. These concentrations of up to 80 g peptone per l are not realistic for large scale-fermentations. Yet high levels of the enzyme produced under these conditions should stimulate both basic studies on the enzyme and studies on potential technological applications, e.g. for biosensors, bioremediation, or biocatalysis Athelia rolfsii

General Stability

EC Number General Stability Organism
1.1.99.18 the enzyme contains a protease-sensitive linker region, can be cleaved by endogenous proteases into a catalytically active flavin fragement and an inactive haem domain. Cleavage can be prevented by using increased concentrations of peptone or certain amino acids such as Val or Leu Athelia rolfsii

Organism

EC Number Organism UniProt Comment Textmining
1.1.99.18 Athelia rolfsii
-
 Athelia, CBS 191.62, very efficient producer of the enzyme
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.99.18 lactose + 2,6-dichlorophenol-indophenol
-
 Athelia rolfsii 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichlorophenol-indophenol
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.99.18 Cdh
-
 Athelia rolfsii

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.99.18 flavin the enzyme contains a protease-sensitive linker region, can be cleaved by endogenous proteases into a catalytically active flavin fragment and an inactive haem domain. Cleavage can be prevented by using increased concentrations of peptone or certain amino acids such as Val or Leu Athelia rolfsii
1.1.99.18 heme the enzyme contains a protease-sensitive linker region, can be cleaved by endogenous proteases into a catalytically active flavin fragment and an inactive haem domain Athelia rolfsii