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Literature summary extracted from

  • Hung, M.N.; Lee, B.H.
    Purification and characterization of a recombinant beta-galactosidase with transgalactosylation activity from Bifidobacterium infantis HL96 (2002), Appl. Microbiol. Biotechnol., 58, 439-445.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.23 expression in Escherichia coli Bifidobacterium longum subsp. infantis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.23 Cr3+
-
 Bifidobacterium longum subsp. infantis
3.2.1.23 EDTA
-
 Bifidobacterium longum subsp. infantis
3.2.1.23 galactose inhibits hydrolysis of lactose Bifidobacterium longum subsp. infantis
3.2.1.23 PCMB
-
 Bifidobacterium longum subsp. infantis
3.2.1.23 Urea
-
 Bifidobacterium longum subsp. infantis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.23 2.6
-
 o-nitrophenyl beta-D-galactopyranoside
-
 Bifidobacterium longum subsp. infantis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.23 K+ 10 mM, required Bifidobacterium longum subsp. infantis
3.2.1.23 Na+ 10 mM, required Bifidobacterium longum subsp. infantis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.23 115000
-
 4 * 115000, SDS-PAGE Bifidobacterium longum subsp. infantis
3.2.1.23 470000
-
 nondenaturing PAGE Bifidobacterium longum subsp. infantis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.23 Bifidobacterium longum subsp. infantis
-
-
-
3.2.1.23 Bifidobacterium longum subsp. infantis HL96
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.23
-
 Bifidobacterium longum subsp. infantis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.23 lactose + H2O
-
 Bifidobacterium longum subsp. infantis D-glucose + D-galactose
-
 ?
3.2.1.23 lactose + H2O
-
 Bifidobacterium longum subsp. infantis HL96 D-glucose + D-galactose
-
 ?
3.2.1.23 additional information strong transgalactosylation activity Bifidobacterium longum subsp. infantis ?
-
 ?
3.2.1.23 additional information strong transgalactosylation activity Bifidobacterium longum subsp. infantis HL96 ?
-
 ?
3.2.1.23 o-nitrophenyl beta-D-galactopyranoside + H2O
-
 Bifidobacterium longum subsp. infantis o-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 o-nitrophenyl beta-D-galactopyranoside + H2O
-
 Bifidobacterium longum subsp. infantis HL96 o-nitrophenol + beta-D-galactose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.23 tetramer 4 * 115000, SDS-PAGE Bifidobacterium longum subsp. infantis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.23 50
-
 hydrolysis of o-nitrophenyl beta-D-galactopyranoside Bifidobacterium longum subsp. infantis
3.2.1.23 60
-
 hydrolysis of lactose Bifidobacterium longum subsp. infantis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.23 50
-
 pH 7.0, 80 min, stable Bifidobacterium longum subsp. infantis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.23 7.5
-
 hydrolysis of lactose or o-nitrophenyl beta-D-galactopyranoside Bifidobacterium longum subsp. infantis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.23 5 8.5 stable over the range Bifidobacterium longum subsp. infantis