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Literature summary extracted from

  • Sheridan, P.P.; Brenchley, J.E.
    Characterization of a salt-tolerant family 42 beta-galactosidase from a psychrophilic antarctic Planococcus isolate (2000), Appl. Environ. Microbiol., 66, 2438-2444.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.23 lactose 10 mM, 29% inhibition at 1.1 mM o-nitrophenyl beta-D-galactopyranoside Planococcus sp. (in: Bacteria)

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.23 additional information
-
 additional information
-
 Planococcus sp. (in: Bacteria)

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.23 KCl still active in presence of 4 M, more active in presence of KCl than in presence of NaCl Planococcus sp. (in: Bacteria)
3.2.1.23 NaCl still active in presence of 4 M, more active in presence of KCl than in presence of NaCl Planococcus sp. (in: Bacteria)

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.23 75000
-
 2 * 75000, SDS-PAGE Planococcus sp. (in: Bacteria)
3.2.1.23 155000
-
 light scattering experiments Planococcus sp. (in: Bacteria)

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.23 Planococcus sp. (in: Bacteria) Q9KI47
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.23
-
 Planococcus sp. (in: Bacteria)

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.23 o-nitrophenyl beta-D-fucopyranoside + H2O 6.1% of the activity with o-nitrophenyl beta-D-galactopyranoside Planococcus sp. (in: Bacteria) o-nitrophenol + beta-D-fucose
-
 ?
3.2.1.23 o-nitrophenyl beta-D-galactopyranoside + H2O
-
 Planococcus sp. (in: Bacteria) o-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 p-nitrophenyl beta-D-fucopyranoside + H2O 3.8% of the activity with o-nitrophenyl beta-D-galactopyranoside Planococcus sp. (in: Bacteria) p-nitrophenol + beta-D-fucose
-
 ?
3.2.1.23 p-nitrophenyl beta-D-galactopyranoside + H2O 93% of the activity with o-nitrophenyl beta-D-galactopyranoside Planococcus sp. (in: Bacteria) p-nitrophenol + beta-D-galactose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.23 dimer 2 * 75000, SDS-PAGE Planococcus sp. (in: Bacteria)

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.23 42
-
-
 Planococcus sp. (in: Bacteria)

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.23 24 56 24°C: about 45% of maximal activity, 56°C: about 70% of maximal activity Planococcus sp. (in: Bacteria)

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.23 42
-
 2 h, stable Planococcus sp. (in: Bacteria)
3.2.1.23 48
-
 1 h, about 35% loss of activity Planococcus sp. (in: Bacteria)
3.2.1.23 55
-
 10 min, complete and irreversible inactivation after 10 min Planococcus sp. (in: Bacteria)

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.23 81.8
-
 o-nitrophenyl beta-D-galactopyranoside 1.9°C Planococcus sp. (in: Bacteria)
3.2.1.23 104
-
 o-nitrophenyl beta-D-galactopyranoside 10°C Planococcus sp. (in: Bacteria)
3.2.1.23 288
-
 o-nitrophenyl beta-D-galactopyranoside 20°C Planococcus sp. (in: Bacteria)
3.2.1.23 507
-
 o-nitrophenyl beta-D-galactopyranoside 30°C Planococcus sp. (in: Bacteria)
3.2.1.23 603
-
 o-nitrophenyl beta-D-galactopyranoside 39°C Planococcus sp. (in: Bacteria)

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.23 6.5
-
-
 Planococcus sp. (in: Bacteria)