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Literature summary extracted from

  • Gill, P.K.; Manhas, R.K.; Singh, J.; Singh, P.
    Purification and characterization of an exoinulinase from Aspergillus fumigatus (2004), Appl. Biochem. Biotechnol., 117, 19-32.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.80 EDTA 1 mM, 63% of inhibition Aspergillus fumigatus
3.2.1.80 Hg2+ 1 mM, 100% of inhibition Aspergillus fumigatus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.80 0.25
-
inulin
-
Aspergillus fumigatus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.80 extracellular
-
Aspergillus fumigatus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.80 59400
-
1 * 66000 + 1 * 62700 + 1 * 59400, SDS-PAGE Aspergillus fumigatus
3.2.1.80 62700
-
1 * 66000 + 1 * 62700 + 1 * 59400, SDS-PAGE Aspergillus fumigatus
3.2.1.80 66000
-
1 * 66000 + 1 * 62700 + 1 * 59400, SDS-PAGE Aspergillus fumigatus
3.2.1.80 176500
-
native PAGE Aspergillus fumigatus
3.2.1.80 200000
-
gel filtration Aspergillus fumigatus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.80 Aspergillus fumigatus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.80 by ammonium sulfate precipitation, DEAE-Sephacel column, Sephacryl S-200 column, concanavalin A-linked amino-activated silica column and Sepharose 6B column, 25fold Aspergillus fumigatus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.80 additional information
-
171 U/mg of protein Aspergillus fumigatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.80 inulin + H2O
-
Aspergillus fumigatus oligofructosides
-
?
3.2.1.80 raffinose + H2O
-
Aspergillus fumigatus beta-D-fructose + alpha-D-galactopyranosyl-1,6-alpha-D-glucopyranoside
-
?
3.2.1.80 stachyose + H2O
-
Aspergillus fumigatus beta-D-fructose + alpha-D-galactopyranosyl-1,6-alpha-D-galactopyranosyl-1,6-alpha-D-galactopyranose
-
?
3.2.1.80 sucrose + H2O
-
Aspergillus fumigatus beta-D-fructose + alpha-D-glucose
-
?

Subunits

EC Number Subunits Comment Organism
3.2.1.80 heterotrimer 1 * 66000 + 1 * 62700 + 1 * 59400, SDS-PAGE Aspergillus fumigatus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.80 exoinulinase
-
Aspergillus fumigatus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.80 60
-
enzyme retains 35.9% and 25.8% activities after 4 h at 50 and 55ºC, respectively Aspergillus fumigatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.80 5.5
-
-
Aspergillus fumigatus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.80 4 9.5
-
Aspergillus fumigatus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.80 Aspergillus fumigatus isoelectric focusing 4.7 4.6