Literature summary extracted from

Loechel, C.; Basran, A.; Basran, J.; Scrutton, N.S.; Hall, E.A.
Using trimethylamine dehydrogenase in an enzyme linked amperometric electrode. Part 1. Wild-type enzyme redox mediation (2003), Analyst, 128, 166-172.

Application

EC Number	Application	Comment	Organism
1.5.8.2	nutrition	quality control, development of an amperometric enzyme electrode sensor for rapid detection of trimethylamine in different fish muscle samples using phenazine methosulfate as redox cofactor and NBT-based colorimetric detectiona nd quantification	Methylophilus methylotrophus

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
1.5.8.2	2,6-dichlorophenolindophenol	-	Methylophilus methylotrophus
1.5.8.2	ferricinium	Val334 is involved in binding and initiates a conformational change interrupting the electron transfer between FMN and the [4Fe-4S] cluster	Methylophilus methylotrophus
1.5.8.2	additional information	no inhibition by (dimethylamino)methylene ferrocene, ferrocenedicarboxylic acid, ferrocenemonocarboxylic acid, ferrocenedimathanol, (dimethylamino)methylene ferrocene, ferricyanide, and thionine	Methylophilus methylotrophus

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.5.8.2	additional information	-	additional information	kinetic mechanism, steady-state kinetics using (dimethylamino)methylene ferrocene, pH-dependency and effects on kinetics, overview	Methylophilus methylotrophus

Metals/Ions

EC Number	Metals/Ions	Comment	Organism	Structure
1.5.8.2	Fe2+	enzyme contains a [4Fe-4S] cluster, electron transfer between FMN and the [4Fe-4S] cluster	Methylophilus methylotrophus

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
1.5.8.2	trimethylamine + H2O + FMN	Methylophilus methylotrophus	-	dimethylamine + formaldehyde + FMNH2	-	?

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.5.8.2	Methylophilus methylotrophus	-	-	-

Reaction

EC Number	Reaction	Comment	Organism	Reaction ID
1.5.8.2	trimethylamine + H2O + electron-transfer flavoprotein = dimethylamine + formaldehyde + reduced electron-transfer flavoprotein	redox mediation mechanism, overview	Methylophilus methylotrophus	a

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.5.8.2	additional information	recycling of the prosthetic electron-transferring group FMN by ferricinium, ferrocenedicarboxylic acid, ferrocenemonocarboxylic acid, ferrocenedimathanol, (dimethylamino)methylene ferrocene, and with lower activity by ferricyanide, no electron-transfer activity with 2,6-dichlorophenolindophenol, and thionine	Methylophilus methylotrophus	?	-	?
1.5.8.2	trimethylamine + H2O + FMN	-	Methylophilus methylotrophus	dimethylamine + formaldehyde + FMNH2	-	?

Synonyms

EC Number	Synonyms	Comment	Organism
1.5.8.2	TMADH	-	Methylophilus methylotrophus

Temperature Optimum [°C]

EC Number	Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
1.5.8.2	25	-	assay at	Methylophilus methylotrophus

pH Optimum

EC Number	pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
1.5.8.2	7	-	assay at	Methylophilus methylotrophus

pH Range

EC Number	pH Minimum	pH Maximum	Comment	Organism
1.5.8.2	6	11	-	Methylophilus methylotrophus

Cofactor

EC Number	Cofactor	Comment	Organism	Structure
1.5.8.2	FMN	-	Methylophilus methylotrophus

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Release 2023.1 (January 2023)