BRENDA - Enzyme Database show

cDNA cloning and mutagenesis study of liver-type prostaglandin F synthase, and identification of the prostaglandin F producing cells in the liver

Suzuki-Yamamoto, T.; Fujii, Y.; Miyano, M.; Chen, L.Y.; Takahashi, T.; Yokoi, H.; Tsuruo, Y.; Ishimura, K.; Watanabe, K.; Adv. Exp. Med. Biol. 507, 263-268 (2002)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.1.1.188
liver-type isozyme, full length cDNA from cDNA library, DNA and amino acid sequence determination and analysis, transient expression of wild-type and mutant enzymes in COS-7 cells
Bos taurus
Engineering
EC Number
Amino acid exchange
Commentary
Organism
1.1.1.188
H170C
site-directed mutagenesis, increased Km for prostaglandin PGD2 and 8fold decreased Km for prostaglandin PGH2 compared to the wild-type enzyme
Bos taurus
1.1.1.188
K225S
site-directed mutagenesis, slightly increased Km for prostaglandin PGD2 and PGH2 compared to the wild-type enzyme
Bos taurus
1.1.1.188
N306Y
site-directed mutagenesis, increased Km for prostaglandin PGD2 and 5fold increased activity with prostaglandin PGH2 compared to the wild-type enzyme
Bos taurus
1.1.1.188
R223L
site-directed mutagenesis, increased Km for prostaglandin PGD2 compared to the wild-type enzyme
Bos taurus
1.1.1.188
R27E
site-directed mutagenesis, increased Km for prostaglandin PGD2 compared to the wild-type enzyme
Bos taurus
1.1.1.188
R91Q
site-directed mutagenesis, increased Km for prostaglandin PGD2 compared to the wild-type enzyme
Bos taurus
1.1.1.188
S301R
site-directed mutagenesis, slightly increased Km for prostaglandin PGD2 and PGH2 compared to the wild-type enzyme
Bos taurus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.188
0.0011
-
9,10-phenanthrenequinone
recombinant wild-type liver-type isozyme, 37°C
Bos taurus
1.1.1.188
0.003
-
prostaglandin PGH2
recombinant liver-type isozyme, mutant H170C, 37°C
Bos taurus
1.1.1.188
0.01
-
prostaglandin PGH2
recombinant liver-type isozyme, mutants R91Q, R223L and N306Y, 37°C
Bos taurus
1.1.1.188
0.014
-
prostaglandin PGH2
recombinant liver-type isozyme, mutant R27E, 37°C
Bos taurus
1.1.1.188
0.015
-
prostaglandin PGD2
recombinant wild-type liver-type isozyme, 37°C
Bos taurus
1.1.1.188
0.025
-
prostaglandin PGH2
recombinant wild-type liver-type isozyme, 37°C
Bos taurus
1.1.1.188
0.028
-
prostaglandin PGH2
recombinant liver-type isozyme, mutant K225S, 37°C
Bos taurus
1.1.1.188
0.03
-
prostaglandin PGH2
recombinant liver-type isozyme, mutant S301R, 37°C
Bos taurus
1.1.1.188
0.04
-
prostaglandin PGD2
recombinant liver-type isozyme mutant S301R, 37°C
Bos taurus
1.1.1.188
0.05
-
prostaglandin PGD2
recombinant liver-type isozyme mutant K225S, 37°C
Bos taurus
1.1.1.188
0.075
-
prostaglandin PGD2
recombinant liver-type isozyme mutant H170C, 37°C
Bos taurus
1.1.1.188
0.1
-
prostaglandin PGD2
recombinant liver-type isozyme mutant N306Y, 37°C
Bos taurus
1.1.1.188
0.11
-
prostaglandin PGD2
recombinant liver-type isozyme mutant R27E, 37°C
Bos taurus
1.1.1.188
0.12
-
prostaglandin PGD2
recombinant wild-type lung-type isozyme
Bos taurus
1.1.1.188
0.145
-
prostaglandin PGD2
recombinant liver-type isozyme mutant R91Q, 37°C
Bos taurus
1.1.1.188
0.18
-
prostaglandin PGD2
recombinant liver-type isozyme mutant R223L, 37°C
Bos taurus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.1.188
cytoplasm
liver-type isozyme
Bos taurus
5737
-
1.1.1.188
additional information
no liver-type isozyme in mitochondrion, nucleus, or Golgi apparatus
Bos taurus
-
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.188
36666
-
1 * 36666, lung-type isozyme, sequence calculation, 1 * 36742, liver-type isozyme, sequence calculation
Bos taurus
1.1.1.188
36670
-
lung-type isozyme, sequence calculation
Bos taurus
1.1.1.188
36740
-
liver-type isozyme, sequence calculation
Bos taurus
1.1.1.188
36742
-
1 * 36666, lung-type isozyme, sequence calculation, 1 * 36742, liver-type isozyme, sequence calculation
Bos taurus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.188
Bos taurus
-
liver- and lung-type isozymes
-
Purification (Commentary)
EC Number
Commentary
Organism
1.1.1.188
recombinant wild-type liver-type isoyzme and mutant enzymes from COS-7 cells, to homogeneity
Bos taurus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.188
endothelium
liver-type isozyme, sinusoidal endothelial cells in the liver
Bos taurus
-
1.1.1.188
liver
liver-type isozyme, sinusoidal endothelial cells
Bos taurus
-
1.1.1.188
lung
lung-type isozyme
Bos taurus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.188
9,10-phenanthrenequinone + NADPH
-
654159
Bos taurus
?
-
-
-
?
1.1.1.188
prostaglandin D2 + NADPH + H+
amino acid residues Arg27, Arg91, His170, Arg223, and Asn306 are involved in substrate binding
654159
Bos taurus
prostaglandin 9alpha,11beta-F2 + NADP+
-
-
-
?
1.1.1.188
prostaglandin H2 + NADPH
His170 is involved in binding, and Asn306 is critical for catalytic activity
654159
Bos taurus
prostaglandin F2alpha + NADP+
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.1.1.188
monomer
1 * 36666, lung-type isozyme, sequence calculation, 1 * 36742, liver-type isozyme, sequence calculation
Bos taurus
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.1.1.188
37
-
assay at
Bos taurus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.188
NADPH
-
Bos taurus
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.188
liver-type isozyme, full length cDNA from cDNA library, DNA and amino acid sequence determination and analysis, transient expression of wild-type and mutant enzymes in COS-7 cells
Bos taurus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.188
NADPH
-
Bos taurus
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
1.1.1.188
H170C
site-directed mutagenesis, increased Km for prostaglandin PGD2 and 8fold decreased Km for prostaglandin PGH2 compared to the wild-type enzyme
Bos taurus
1.1.1.188
K225S
site-directed mutagenesis, slightly increased Km for prostaglandin PGD2 and PGH2 compared to the wild-type enzyme
Bos taurus
1.1.1.188
N306Y
site-directed mutagenesis, increased Km for prostaglandin PGD2 and 5fold increased activity with prostaglandin PGH2 compared to the wild-type enzyme
Bos taurus
1.1.1.188
R223L
site-directed mutagenesis, increased Km for prostaglandin PGD2 compared to the wild-type enzyme
Bos taurus
1.1.1.188
R27E
site-directed mutagenesis, increased Km for prostaglandin PGD2 compared to the wild-type enzyme
Bos taurus
1.1.1.188
R91Q
site-directed mutagenesis, increased Km for prostaglandin PGD2 compared to the wild-type enzyme
Bos taurus
1.1.1.188
S301R
site-directed mutagenesis, slightly increased Km for prostaglandin PGD2 and PGH2 compared to the wild-type enzyme
Bos taurus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.188
0.0011
-
9,10-phenanthrenequinone
recombinant wild-type liver-type isozyme, 37°C
Bos taurus
1.1.1.188
0.003
-
prostaglandin PGH2
recombinant liver-type isozyme, mutant H170C, 37°C
Bos taurus
1.1.1.188
0.01
-
prostaglandin PGH2
recombinant liver-type isozyme, mutants R91Q, R223L and N306Y, 37°C
Bos taurus
1.1.1.188
0.014
-
prostaglandin PGH2
recombinant liver-type isozyme, mutant R27E, 37°C
Bos taurus
1.1.1.188
0.015
-
prostaglandin PGD2
recombinant wild-type liver-type isozyme, 37°C
Bos taurus
1.1.1.188
0.025
-
prostaglandin PGH2
recombinant wild-type liver-type isozyme, 37°C
Bos taurus
1.1.1.188
0.028
-
prostaglandin PGH2
recombinant liver-type isozyme, mutant K225S, 37°C
Bos taurus
1.1.1.188
0.03
-
prostaglandin PGH2
recombinant liver-type isozyme, mutant S301R, 37°C
Bos taurus
1.1.1.188
0.04
-
prostaglandin PGD2
recombinant liver-type isozyme mutant S301R, 37°C
Bos taurus
1.1.1.188
0.05
-
prostaglandin PGD2
recombinant liver-type isozyme mutant K225S, 37°C
Bos taurus
1.1.1.188
0.075
-
prostaglandin PGD2
recombinant liver-type isozyme mutant H170C, 37°C
Bos taurus
1.1.1.188
0.1
-
prostaglandin PGD2
recombinant liver-type isozyme mutant N306Y, 37°C
Bos taurus
1.1.1.188
0.11
-
prostaglandin PGD2
recombinant liver-type isozyme mutant R27E, 37°C
Bos taurus
1.1.1.188
0.12
-
prostaglandin PGD2
recombinant wild-type lung-type isozyme
Bos taurus
1.1.1.188
0.145
-
prostaglandin PGD2
recombinant liver-type isozyme mutant R91Q, 37°C
Bos taurus
1.1.1.188
0.18
-
prostaglandin PGD2
recombinant liver-type isozyme mutant R223L, 37°C
Bos taurus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.1.188
cytoplasm
liver-type isozyme
Bos taurus
5737
-
1.1.1.188
additional information
no liver-type isozyme in mitochondrion, nucleus, or Golgi apparatus
Bos taurus
-
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.188
36666
-
1 * 36666, lung-type isozyme, sequence calculation, 1 * 36742, liver-type isozyme, sequence calculation
Bos taurus
1.1.1.188
36670
-
lung-type isozyme, sequence calculation
Bos taurus
1.1.1.188
36740
-
liver-type isozyme, sequence calculation
Bos taurus
1.1.1.188
36742
-
1 * 36666, lung-type isozyme, sequence calculation, 1 * 36742, liver-type isozyme, sequence calculation
Bos taurus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.188
recombinant wild-type liver-type isoyzme and mutant enzymes from COS-7 cells, to homogeneity
Bos taurus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.188
endothelium
liver-type isozyme, sinusoidal endothelial cells in the liver
Bos taurus
-
1.1.1.188
liver
liver-type isozyme, sinusoidal endothelial cells
Bos taurus
-
1.1.1.188
lung
lung-type isozyme
Bos taurus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.188
9,10-phenanthrenequinone + NADPH
-
654159
Bos taurus
?
-
-
-
?
1.1.1.188
prostaglandin D2 + NADPH + H+
amino acid residues Arg27, Arg91, His170, Arg223, and Asn306 are involved in substrate binding
654159
Bos taurus
prostaglandin 9alpha,11beta-F2 + NADP+
-
-
-
?
1.1.1.188
prostaglandin H2 + NADPH
His170 is involved in binding, and Asn306 is critical for catalytic activity
654159
Bos taurus
prostaglandin F2alpha + NADP+
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.1.1.188
monomer
1 * 36666, lung-type isozyme, sequence calculation, 1 * 36742, liver-type isozyme, sequence calculation
Bos taurus
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.1.1.188
37
-
assay at
Bos taurus