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Literature summary extracted from

  • Cheung, Y.Y.; Allen, M.D.; Bycroft, M.; Wong, K.B.
    Crystallization and preliminary crystallographic analysis of an acylphosphatase from the hyperthermophilic archaeon Pyrococcus horikoshii (2004), Acta Crystallogr. Sect. D, 60, 1308-1310.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.7
-
 Pyrococcus horikoshii
3.6.1.7 expressed in Escherichia coli strain C41 Pyrococcus horikoshii

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.6.1.7 sitting drop vapour diffusion method with sodium formate as precipitant Pyrococcus horikoshii
3.6.1.7 sitting-drop vapour-diffusion method using sodium formate as precipitant at 16°C. The crystals belong to space group P3(2)21, with unit-cell parameters a = b = 85.65 A, c = 75.51 A. The asymmetric unit contains two molecules of enzyme Pyrococcus horikoshii

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.7 Pyrococcus horikoshii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.7
-
 Pyrococcus horikoshii
3.6.1.7 HiTrap SP HP column chromatography and Superdex 75 column chromatography Pyrococcus horikoshii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.7 1,3-diphosphoglycerate + H2O
-
 Pyrococcus horikoshii 3-phosphoglycerate + phosphate
-
 ?
3.6.1.7 acetyl phosphate + H2O
-
 Pyrococcus horikoshii acetate + phosphate
-
 ?
3.6.1.7 carbamoyl phosphate + H2O
-
 Pyrococcus horikoshii carbamate + phosphate
-
 ?
3.6.1.7 succinyl phosphate + H2O
-
 Pyrococcus horikoshii succinate + phosphate
-
 ?