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Literature summary extracted from

  • Miyazono, K.I.; Kudo, N.; Tanokura, M.
    Cloning, purification, crystallization and preliminary crystallographic analysis of acylphosphatase from Pyrococcus horikoshii OT3 (2004), Acta Crystallogr. Sect. D, 60, 1135-1136.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.7 expressed in Escherichia coli strain Rosetta Pyrococcus horikoshii
3.6.1.7 expression in Escherichia coli Pyrococcus horikoshii

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.6.1.7 sitting drop vapour diffusion method with potassium/sodium tartrate at pH 5.5 Pyrococcus horikoshii
3.6.1.7 sitting-drop vapour-diffusion method with postasium/sodium tartrate as the precipitant at pH 5.5. The crystals belong to space group P3(2)21, with approximate unit-cell parameters a = b = 86.7 A, c = 75.4 A and two monomers in the asymmetric unit Pyrococcus horikoshii

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.7 Pyrococcus horikoshii
-
 strain OT3
-
3.6.1.7 Pyrococcus horikoshii OT-3
-
 strain OT3
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.7
-
 Pyrococcus horikoshii
3.6.1.7 ammonium sulfate fractionation and cation exchange chromatography Pyrococcus horikoshii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.7 1,3-diphosphoglycerate + H2O
-
 Pyrococcus horikoshii 3-phosphoglycerate + phosphate
-
 ?
3.6.1.7 1,3-diphosphoglycerate + H2O
-
 Pyrococcus horikoshii OT-3 3-phosphoglycerate + phosphate
-
 ?
3.6.1.7 acetyl phosphate + H2O
-
 Pyrococcus horikoshii acetate + phosphate
-
 ?
3.6.1.7 acetyl phosphate + H2O
-
 Pyrococcus horikoshii OT-3 acetate + phosphate
-
 ?
3.6.1.7 benzoyl phosphate + H2O
-
 Pyrococcus horikoshii benzoate + phosphate
-
 ?
3.6.1.7 benzoyl phosphate + H2O
-
 Pyrococcus horikoshii OT-3 benzoate + phosphate
-
 ?
3.6.1.7 beta-aspartyl phosphate + H2O
-
 Pyrococcus horikoshii aspartate + phosphate
-
 ?
3.6.1.7 beta-aspartyl phosphate + H2O
-
 Pyrococcus horikoshii OT-3 aspartate + phosphate
-
 ?
3.6.1.7 carbamoyl phosphate + H2O
-
 Pyrococcus horikoshii carbamate + phosphate
-
 ?
3.6.1.7 carbamoyl phosphate + H2O
-
 Pyrococcus horikoshii OT-3 carbamate + phosphate
-
 ?
3.6.1.7 succinyl phosphate + H2O
-
 Pyrococcus horikoshii succinate + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.6.1.7 ACP
-
 Pyrococcus horikoshii