Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Horsefield, R.; Yankovskaya, V.; Tornroth, S.; Luna-Chavez, C.; Stambouli, E.; Barber, J.; Byrne, B.; Cecchini, G.; Iwata, S.
    Using rational screening and electron microscopy to optimize the crystallization of succinate:ubiquinone oxidoreductase from Escherichia coli (2003), Acta Crystallogr. Sect. D, 59, 600-602.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.5.1 expression in Escherichia coli Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.3.5.1 hanging-drop vapour-diffusion, SQR in 20 mM Tris-HCl, pH 7.6, 0.05% THESIT is mixed with an equal volume of reservoir solution containing 100 mM Na-HEPES, pH 7.5, 200 mM, CaCl2 and 28% polyethylene glycol 400, crystals diffract to 2.6 A resolution Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.3.5.1 membrane membrane bound Escherichia coli 16020
-
1.3.5.1 mitochondrion
-
 Escherichia coli 5739
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.5.1 succinate + ubiquinone Escherichia coli
-
 fumarate + ubiquinol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.5.1 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.5.1 recombinant SQR, Q-Sepharose, Poros 50HQ, Sephacryl S-300 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.5.1 succinate + ubiquinone
-
 Escherichia coli fumarate + ubiquinol
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.5.1 SQR
-
 Escherichia coli