Literature summary extracted from
Hagemeier, C.H.; Shima, S.; Warkentin, E.; Thauer, R.K.; Ermler, U.
Coenzyme F420-dependent methylenetetrahydromethanopterin dehydrogenase from Methanopyrus kandleri: the selenomethionine-labelled and non-labelled enzyme crystallized in two different forms (2003), Acta Crystallogr. Sect. D, 59, 1653-1655.
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
1.5.98.1 |
purified recombinant selenomethionine-labeled enzyme, hanging drop vapour diffusion method, 0.001 ml protein solution containing 12 mg/ml enzyme and 10 mM MOPS-KOH, pH 7.0, mixed with 0.001 ml reservoir solution containing 13% v/v 2-methyl-2,4-pentanediol, 0.1 M sodium phosphate, pH 8.0, and 0.2 M magnesium acetate, X-ray diffraction structure determination and analysis at 1.54 A resolution by single wavelength anomalous dispersion method, or at 2.4 A resolution by multiwavelength anomalous dispersion method |
Methanopyrus kandleri |
KM Value [mM]
EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Comment |
Organism |
Structure |
---|
1.5.98.1 |
additional information |
- |
additional information |
ternary complex kinetic mechanism |
Methanopyrus kandleri |
|
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
1.5.98.1 |
5,10-methylenetetrahydromethanopterin + oxidized coenzyme F420 |
Methanopyrus kandleri |
fourth step in the CO2 reduction to methane |
5,10-methenyltetrahydromethanopterin + reduced coenzyme F420 |
- |
r |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
1.5.98.1 |
Methanopyrus kandleri |
- |
methanogenic archeon |
- |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
1.5.98.1 |
5,10-methylenetetrahydromethanopterin + oxidized coenzyme F420 |
fourth step in the CO2 reduction to methane |
Methanopyrus kandleri |
5,10-methenyltetrahydromethanopterin + reduced coenzyme F420 |
- |
r |
|
1.5.98.1 |
5,10-methylenetetrahydromethanopterin + oxidized coenzyme F420 |
substrate binding site structure analysis |
Methanopyrus kandleri |
5,10-methenyltetrahydromethanopterin + reduced coenzyme F420 |
- |
r |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
1.5.98.1 |
hexamer |
6 * 30000-35000, homohexamer build as a trimer of dimers, each subunit has 2 domains, a larger alphabeta domain and a smaller helix bundle domain, quarternary structure from crystal strutcure analysis |
Methanopyrus kandleri |
1.5.98.1 |
More |
structural adaption to extreme environment |
Methanopyrus kandleri |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
1.5.98.1 |
MTD |
- |
Methanopyrus kandleri |
Cofactor
EC Number |
Cofactor |
Comment |
Organism |
Structure |
---|
1.5.98.1 |
coenzyme F420 |
dependent on, binding site structure |
Methanopyrus kandleri |
|
1.5.98.1 |
additional information |
enzyme contains no prosthetic group |
Methanopyrus kandleri |
|