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Literature summary extracted from

  • Hagemeier, C.H.; Shima, S.; Warkentin, E.; Thauer, R.K.; Ermler, U.
    Coenzyme F420-dependent methylenetetrahydromethanopterin dehydrogenase from Methanopyrus kandleri: the selenomethionine-labelled and non-labelled enzyme crystallized in two different forms (2003), Acta Crystallogr. Sect. D, 59, 1653-1655.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.5.98.1 purified recombinant selenomethionine-labeled enzyme, hanging drop vapour diffusion method, 0.001 ml protein solution containing 12 mg/ml enzyme and 10 mM MOPS-KOH, pH 7.0, mixed with 0.001 ml reservoir solution containing 13% v/v 2-methyl-2,4-pentanediol, 0.1 M sodium phosphate, pH 8.0, and 0.2 M magnesium acetate, X-ray diffraction structure determination and analysis at 1.54 A resolution by single wavelength anomalous dispersion method, or at 2.4 A resolution by multiwavelength anomalous dispersion method Methanopyrus kandleri

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.98.1 additional information
-
additional information ternary complex kinetic mechanism Methanopyrus kandleri

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.98.1 5,10-methylenetetrahydromethanopterin + oxidized coenzyme F420 Methanopyrus kandleri fourth step in the CO2 reduction to methane 5,10-methenyltetrahydromethanopterin + reduced coenzyme F420
-
r

Organism

EC Number Organism UniProt Comment Textmining
1.5.98.1 Methanopyrus kandleri
-
methanogenic archeon
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.98.1 5,10-methylenetetrahydromethanopterin + oxidized coenzyme F420 fourth step in the CO2 reduction to methane Methanopyrus kandleri 5,10-methenyltetrahydromethanopterin + reduced coenzyme F420
-
r
1.5.98.1 5,10-methylenetetrahydromethanopterin + oxidized coenzyme F420 substrate binding site structure analysis Methanopyrus kandleri 5,10-methenyltetrahydromethanopterin + reduced coenzyme F420
-
r

Subunits

EC Number Subunits Comment Organism
1.5.98.1 hexamer 6 * 30000-35000, homohexamer build as a trimer of dimers, each subunit has 2 domains, a larger alphabeta domain and a smaller helix bundle domain, quarternary structure from crystal strutcure analysis Methanopyrus kandleri
1.5.98.1 More structural adaption to extreme environment Methanopyrus kandleri

Synonyms

EC Number Synonyms Comment Organism
1.5.98.1 MTD
-
Methanopyrus kandleri

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.98.1 coenzyme F420 dependent on, binding site structure Methanopyrus kandleri
1.5.98.1 additional information enzyme contains no prosthetic group Methanopyrus kandleri