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Literature summary extracted from

  • Couture, J.F.; Cantin, L.; Legrand, P.; Luu-The, V.; Labrie, F.; Breton, R.
    Expression, crystallization and preliminary X-ray analysis of human and rabbit 20alpha-hydroxysteroid dehydrogenases in complex with NADP(H) and various steroid substrates (2002), Acta Crystallogr. Sect. D, 58, 135-139.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.149 expression of soluble, active enzyme as GST-fusion enzyme in Escherichia coli Homo sapiens
1.1.1.149 expression of soluble, active enzyme as GST-fusion enzyme in Escherichia coli Oryctolagus cuniculus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.149 in complex with NADP+ and progesterone Homo sapiens
1.1.1.149 in complex with NADP+ and progesterone, with NADP+ and dihydrotestosterone, with NADP+ and 4-androstenedione Oryctolagus cuniculus
1.1.1.149 purified recombinant enzyme in complex with cofactor and several substrates, hanging drop vapour diffusion method, 4°C, HEPES or sodium acetate buffer, CaCl2, precipitant is PEG 4000, X-ray diffraction structure determination and analysis at 1.7-1.9 A resolution Oryctolagus cuniculus
1.1.1.149 purified recombinant enzyme in complex with cofactor and several substrates, hanging drop vapour diffusion method, 4°C, HEPES or sodium acetate buffer, CaCl2, precipitant is PEG 4000, X-ray diffraction structure determination and analysis at 2.4-2.5 A resolution Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.149 17alpha-hydroxyprogesterone + NAD(P)H Homo sapiens
-
 17alpha,20alpha-dihydroxypregn-4-en-3-one + NAD(P)+
-
 r
1.1.1.149 17alpha-hydroxyprogesterone + NAD(P)H Oryctolagus cuniculus
-
 17alpha,20alpha-dihydroxypregn-4-en-3-one + NAD(P)+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.149 Homo sapiens
-
-
-
1.1.1.149 Oryctolagus cuniculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.149 recombinant GST-fusion enzyme by glutahione affinity chromatography and cleavage of the GST-tag by Factor Xa, followed by ion exchange chromatography, and gel filtration, to homogeneity Homo sapiens
1.1.1.149 recombinant GST-fusion enzyme by glutahione affinity chromatography and cleavage of the GST-tag by Factor Xa, followed by ion exchange chromatography, and gel filtration, to homogeneity Oryctolagus cuniculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.149 17alpha-hydroxyprogesterone + NAD(P)H
-
 Homo sapiens 17alpha,20alpha-dihydroxypregn-4-en-3-one + NAD(P)+
-
 r
1.1.1.149 17alpha-hydroxyprogesterone + NAD(P)H
-
 Oryctolagus cuniculus 17alpha,20alpha-dihydroxypregn-4-en-3-one + NAD(P)+
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.1.1.149 20alpha-HSD
-
 Homo sapiens
1.1.1.149 20alpha-HSD
-
 Oryctolagus cuniculus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.149 37
-
 assay at Homo sapiens
1.1.1.149 37
-
 assay at Oryctolagus cuniculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.149 7.5
-
 assay at Homo sapiens
1.1.1.149 7.5
-
 assay at Oryctolagus cuniculus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.149 NAD(P)+
-
 Homo sapiens
1.1.1.149 NAD(P)+
-
 Oryctolagus cuniculus
1.1.1.149 NAD(P)H
-
 Homo sapiens
1.1.1.149 NAD(P)H
-
 Oryctolagus cuniculus