EC Number | Cloned (Comment) | Organism |
---|---|---|
5.3.1.6 | overexpression in Escherichia coli | Pyrococcus horikoshii |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
5.3.1.6 | hanging drop vapor diffusion method, crystal structure of the free enzyme and the complex with D-4-phosphoerythronic acid | Pyrococcus horikoshii |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.3.1.6 | additional information | - |
additional information | dramatic increase of Km-value at temperatures above 80°C | Pyrococcus horikoshii | |
5.3.1.6 | 0.17 | - |
D-ribose 5-phosphate | 50°C, pH 6.0, mutant enzyme D85N | Pyrococcus horikoshii | |
5.3.1.6 | 1.17 | - |
D-ribose 5-phosphate | 50°C, pH 6.0, wild-type enzyme | Pyrococcus horikoshii | |
5.3.1.6 | 2.39 | - |
D-ribose 5-phosphate | 50°C, pH 6.0, mutant enzyme D168N | Pyrococcus horikoshii | |
5.3.1.6 | 5.1 | - |
D-ribose 5-phosphate | 50°C, pH 6.0, mutant enzyme K125A | Pyrococcus horikoshii | |
5.3.1.6 | 7.13 | - |
D-ribose 5-phosphate | 50°C, pH 6.0, mutant enzyme R100A | Pyrococcus horikoshii |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
5.3.1.6 | 25163 | - |
4 * 25163, calculation from nucleotide sequence | Pyrococcus horikoshii |
5.3.1.6 | 26000 | - |
4 * 26000, in crystal and in solution, each monomer has a new fold consisting of two alpha/beta domains, SDS-PAGE | Pyrococcus horikoshii |
5.3.1.6 | 98000 | - |
gel filtration | Pyrococcus horikoshii |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.3.1.6 | D-ribose 5-phosphate | Pyrococcus horikoshii | - |
D-ribulose 5-phosphate | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
5.3.1.6 | Pyrococcus horikoshii | O50083 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
5.3.1.6 | - |
Pyrococcus horikoshii |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.3.1.6 | D-ribose 5-phosphate | - |
Pyrococcus horikoshii | D-ribulose 5-phosphate | - |
r | |
5.3.1.6 | D-ribose 5-phosphate | direct or indirect catalytic role for the residues E107, D85 and K98 | Pyrococcus horikoshii | D-ribulose 5-phosphate | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
5.3.1.6 | tetramer | 4 * 25163, calculation from nucleotide sequence | Pyrococcus horikoshii |
5.3.1.6 | tetramer | 4 * 26000, in crystal and in solution, each monomer has a new fold consisting of two alpha/beta domains, SDS-PAGE | Pyrococcus horikoshii |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
5.3.1.6 | 95 | - |
- |
Pyrococcus horikoshii |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
5.3.1.6 | 100 | - |
stability and integrity up to, needs at least 250 mM NaCl to maintain its hyperthermostability | Pyrococcus horikoshii |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.3.1.6 | 8.3 | - |
D-ribose 5-phosphate | 50°C, pH 6.0, mutant enzyme D85N | Pyrococcus horikoshii | |
5.3.1.6 | 50 | - |
D-ribulose 5-phosphate | 50°C, pH 6.0, wild-type enzyme | Pyrococcus horikoshii | |
5.3.1.6 | 151 | - |
D-ribose 5-phosphate | 50°C, pH 6.0, mutant enzyme K125A | Pyrococcus horikoshii | |
5.3.1.6 | 177 | - |
D-ribose 5-phosphate | 50°C, pH 6.0, mutant enzyme R100A | Pyrococcus horikoshii | |
5.3.1.6 | 312 | - |
D-ribose 5-phosphate | 50°C, pH 6.0, mutant enzyme D168N | Pyrococcus horikoshii | |
5.3.1.6 | 320 | - |
D-ribose 5-phosphate | 50°C, pH 6.0, wild-type enzyme | Pyrococcus horikoshii | |
5.3.1.6 | 625 | - |
D-ribose 5-phosphate | 93°C, pH 6, wild-type enzyme | Pyrococcus horikoshii |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
5.3.1.6 | 6 | - |
at 50°C | Pyrococcus horikoshii |