Literature summary extracted from

Levy, C.W.; Buckley, P.A.; Sedelnikova, S.; Kato, Y.; Asano, Y.; Rice, D.W.; Baker, P.J.
Insights into enzyme evolution revealed by the structure of methylaspartate ammonia lyase (2002), Structure, 10, 105-113.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
4.3.1.2	expression in Escherichia coli	Citrobacter amalonaticus

Crystallization (Commentary)

EC Number	Crystallization (Comment)	Organism
4.3.1.2	hanging drop vapour diffusion, well solution consists of 100 mM potassium phosphate, pH 8.0, and 32% ammonium sulfate, crystals diffract to 1.3 A	Citrobacter amalonaticus

Metals/Ions

EC Number	Metals/Ions	Comment	Organism	Structure
4.3.1.2	K+	required for activity	Citrobacter amalonaticus
4.3.1.2	Mg2+	required for actiivty	Citrobacter amalonaticus

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
4.3.1.2	45500	-	2 * 45500	Citrobacter amalonaticus

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
4.3.1.2	L-threo-3-methylaspartate	Citrobacter amalonaticus	-	mesaconate + NH3	-	r

Organism

EC Number	Organism	UniProt	Comment	Textmining
4.3.1.2	Citrobacter amalonaticus	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
4.3.1.2	recombinant 3-methylaspartase	Citrobacter amalonaticus

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4.3.1.2	(2S,3S)-3-ethylaspartate	-	Citrobacter amalonaticus	?	-	?
4.3.1.2	L-threo-3-methylaspartate	-	Citrobacter amalonaticus	mesaconate + NH3	-	r

Subunits

EC Number	Subunits	Comment	Organism
4.3.1.2	dimer	2 * 45500	Citrobacter amalonaticus

Synonyms

EC Number	Synonyms	Comment	Organism
4.3.1.2	MAL	-	Citrobacter amalonaticus

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Release 2023.1 (January 2023)