Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Wilkinson, A.; Sayer, H.; Bullard, D.; Smith, A.; Day, J.; Kieser, T.; Bowater, R.
    NAD+-dependent DNA ligases of Mycobacterium tuberculosis and Streptomyces coelicolor (2003), Proteins Struct. Funct. Genet., 51, 321-326.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.5.1.2 expressed in Escherichia coli GR501, that contains a temperature sensitive mutation in ligA and is therefore unable to grow above 42°C, expression of protein enables Escherichia coli GR501 at 44°C Mycobacterium tuberculosis
6.5.1.2 expressed in Escherichia coli GR501, that contains a temperature sensitive mutation in ligA and is therefore unable to grow above 42°C, expression of protein enables Escherichia coli GR501 at 44°C Streptomyces coelicolor
6.5.1.2 expressed in Escherichia coli GR501, that contains a temperature sensitive mutation in ligA and is therefore unable to grow above 42°C, expression of protein enables Escherichia coli GR501 to grow at 44°C Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m Escherichia coli DNA-repair, phosphodiester bond formation between adjacent 5'-phosphate and 3'-hydroxyl groups in double-stranded DNA AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
 ?
6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m Mycobacterium tuberculosis DNA-repair, phosphodiester bond formation between adjacent 5'-phosphate and 3'-hydroxyl groups in double-stranded DNA AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
 ?
6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m Streptomyces coelicolor DNA-repair, phosphodiester bond formation between adjacent 5'-phosphate and 3'-hydroxyl groups in double-stranded DNA AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
 ?
6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m Streptomyces coelicolor A3(2) DNA-repair, phosphodiester bond formation between adjacent 5'-phosphate and 3'-hydroxyl groups in double-stranded DNA AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
 ?
6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m Mycobacterium tuberculosis H37Rv DNA-repair, phosphodiester bond formation between adjacent 5'-phosphate and 3'-hydroxyl groups in double-stranded DNA AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.5.1.2 Escherichia coli
-
-
-
6.5.1.2 Mycobacterium tuberculosis
-
-
-
6.5.1.2 Mycobacterium tuberculosis H37Rv
-
-
-
6.5.1.2 Streptomyces coelicolor
-
-
-
6.5.1.2 Streptomyces coelicolor A3(2)
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.5.1.2 recombinant protein from Escherichia coli GR501 using His-tag Escherichia coli
6.5.1.2 recombinant protein from Escherichia coli GR501 using His-tag Mycobacterium tuberculosis
6.5.1.2 recombinant protein from Escherichia coli GR501 using His-tag Streptomyces coelicolor

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m
-
 Escherichia coli AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
 ?
6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m
-
 Mycobacterium tuberculosis AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
 ?
6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m
-
 Streptomyces coelicolor AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
 ?
6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m DNA-repair, phosphodiester bond formation between adjacent 5'-phosphate and 3'-hydroxyl groups in double-stranded DNA Escherichia coli AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
 ?
6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m DNA-repair, phosphodiester bond formation between adjacent 5'-phosphate and 3'-hydroxyl groups in double-stranded DNA Mycobacterium tuberculosis AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
 ?
6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m DNA-repair, phosphodiester bond formation between adjacent 5'-phosphate and 3'-hydroxyl groups in double-stranded DNA Streptomyces coelicolor AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
 ?
6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m
-
 Streptomyces coelicolor A3(2) AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
 ?
6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m DNA-repair, phosphodiester bond formation between adjacent 5'-phosphate and 3'-hydroxyl groups in double-stranded DNA Streptomyces coelicolor A3(2) AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
 ?
6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m
-
 Mycobacterium tuberculosis H37Rv AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
 ?
6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m DNA-repair, phosphodiester bond formation between adjacent 5'-phosphate and 3'-hydroxyl groups in double-stranded DNA Mycobacterium tuberculosis H37Rv AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
6.5.1.2 NAD+ required Escherichia coli
6.5.1.2 NAD+ required Mycobacterium tuberculosis
6.5.1.2 NAD+ required Streptomyces coelicolor