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Literature summary extracted from

  • Banbula, A.; Potempa, J.; Travis, J.; Bode, W.; Medrano, F.J.
    Crystallization and preliminary X-ray diffraction analysis of gingipain R2 from Porphyromonas gingivalis in complex with H-D-Phe-Phe-Arg-chloromethylketone (1998), Protein Sci., 7, 1259-1261.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.4.22.37 purified enzyme gingipain R2 in complex with fast acting, irreversible inhibitor H-D-Phe-Phe-Arg-chloromethylketone, activated by incubation for 30 min in 0.1 M HEPES, pH 8.0, 10 mM L-cysteine, 2.5 mM CaCl2, at 37°C, alkylation of the active site cysteine by addition of inhibitor, crystallization by sitting-drop vapour diffusion method at 20°C, different protein-inhibitor complex solution systems using PEG 8000 as precipitant for 10 mg/ml protein, X-ray diffraction structure determination at 2.9 A resolution and analysis Porphyromonas gingivalis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.37 D-Phe-Phe-Arg-chloromethylketone fast acting, irreversible alkylating inhibitor Porphyromonas gingivalis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.22.37 protein + H2O Porphyromonas gingivalis
-
 peptides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.37 Porphyromonas gingivalis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.37
-
 Porphyromonas gingivalis

Reaction

EC Number Reaction Comment Organism Reaction ID
3.4.22.37 hydrolysis of proteins and small molecule substrates, with a preference for Arg in P1 active site structure Porphyromonas gingivalis
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.37 protein + H2O
-
 Porphyromonas gingivalis peptides
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.22.37 gingipain R2
-
 Porphyromonas gingivalis
3.4.22.37 RGP-2
-
 Porphyromonas gingivalis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.4.22.37 Porphyromonas gingivalis gingipain R2 isozyme II, isoelectric focusing
-
 5.1