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Literature summary extracted from

  • Stieglitz, K.A.; Seaton, B.A.; Head, J.F.; Stec, B.; Roberts, M.F.
    Unexpected similarity in regulation between an archaeal inositol monophosphatase/fructose bisphosphatase and chloroplast fructose bisphosphatase (2003), Protein Sci., 12, 760-767.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.25
-
 Methanocaldococcus jannaschii
3.1.3.25 expressed in Escherichia coli Archaeoglobus fulgidus

Protein Variants

EC Number Protein Variants Comment Organism
3.1.3.25 C150S insensitive toward inactivation with oxygen or thioredoxin, no effect on activity Archaeoglobus fulgidus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.25 O2 activity of the native enzyme but not the C150S mutant can be completely abolished with oxygen, reversible with thiol-containing compounds Archaeoglobus fulgidus
3.1.3.25 thioredoxin inhibits native enzyme but nut C150S mutant Archaeoglobus fulgidus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.25 3.8
-
 4-nitrophenyl phosphate native enzyme, pH 8.0, 80°C Archaeoglobus fulgidus
3.1.3.25 4.1
-
 4-nitrophenyl phosphate C150S mutant enzyme, pH 8.0, 80°C Archaeoglobus fulgidus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.25 28000
-
 2 * 28000 Archaeoglobus fulgidus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.25 myo-inositol 1-phosphate + H2O Methanocaldococcus jannaschii
-
 myo-inositol + phosphate
-
 ?
3.1.3.25 myo-inositol 1-phosphate + H2O Archaeoglobus fulgidus
-
 myo-inositol + phosphate
-
 ?
3.1.3.25 myo-inositol 1-phosphate + H2O Methanocaldococcus jannaschii MJ0109
-
 myo-inositol + phosphate
-
 ?
3.1.3.25 myo-inositol 1-phosphate + H2O Archaeoglobus fulgidus AF2372
-
 myo-inositol + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.25 Archaeoglobus fulgidus
-
-
-
3.1.3.25 Archaeoglobus fulgidus AF2372
-
-
-
3.1.3.25 Methanocaldococcus jannaschii
-
-
-
3.1.3.25 Methanocaldococcus jannaschii MJ0109
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.25
-
 Methanocaldococcus jannaschii
3.1.3.25
-
 Archaeoglobus fulgidus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.25 4-nitrophenyl phosphate + H2O
-
 Archaeoglobus fulgidus 4-nitrophenol + phosphate
-
 ?
3.1.3.25 4-nitrophenyl phosphate + H2O
-
 Archaeoglobus fulgidus AF2372 4-nitrophenol + phosphate
-
 ?
3.1.3.25 D-fructose 1,6-bisphosphate + H2O
-
 Methanocaldococcus jannaschii D-fructose 6-phosphate + phosphate
-
 ?
3.1.3.25 D-fructose 1,6-bisphosphate + H2O
-
 Archaeoglobus fulgidus D-fructose 6-phosphate + phosphate
-
 ?
3.1.3.25 D-fructose 1,6-bisphosphate + H2O
-
 Methanocaldococcus jannaschii MJ0109 D-fructose 6-phosphate + phosphate
-
 ?
3.1.3.25 D-fructose 1,6-bisphosphate + H2O
-
 Archaeoglobus fulgidus AF2372 D-fructose 6-phosphate + phosphate
-
 ?
3.1.3.25 additional information archeal enzyme displays inositol monophosphatase as well as fructose 1,6-bisphosphatase activity Archaeoglobus fulgidus ?
-
 ?
3.1.3.25 additional information archeal enzyme displays inositol monophosphatase as well as fructose 1,6-bisphosphatase activity Archaeoglobus fulgidus AF2372 ?
-
 ?
3.1.3.25 myo-inositol 1-phosphate + H2O
-
 Methanocaldococcus jannaschii myo-inositol + phosphate
-
 ?
3.1.3.25 myo-inositol 1-phosphate + H2O
-
 Archaeoglobus fulgidus myo-inositol + phosphate
-
 ?
3.1.3.25 myo-inositol 1-phosphate + H2O
-
 Methanocaldococcus jannaschii MJ0109 myo-inositol + phosphate
-
 ?
3.1.3.25 myo-inositol 1-phosphate + H2O
-
 Archaeoglobus fulgidus AF2372 myo-inositol + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.25 dimer 2 * 28000 Archaeoglobus fulgidus

Synonyms

EC Number Synonyms Comment Organism
3.1.3.25 More archeal enzyme displays inositol monophosphatase as well as fructose 1,6-bisphosphatase activity Methanocaldococcus jannaschii