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Literature summary extracted from

  • Choi, G.S.; Kim, J.Y.; Kim, J.H.; Ryu, Y.W.; Kim, G.J.
    Construction and characterization of a recombinant esterase with high activity and enantioselectivity to (S)-ketoprofen ethyl ester (2003), Protein Expr. Purif., 29, 85-93.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.1 EDTA slight activation Pseudomonas fluorescens
3.1.1.1 Triton X-100 11fold increased activity Pseudomonas fluorescens
3.1.1.1 Triton X-15 3.3fold increased activity Pseudomonas fluorescens
3.1.1.1 Triton X-165 9.5fold increased activity Pseudomonas fluorescens
3.1.1.1 Triton X-305 10.5fold increased activity Pseudomonas fluorescens
3.1.1.1 Triton X-45 9.4fold increased activity Pseudomonas fluorescens
3.1.1.1 Tween 20 1.7fold increased activity Pseudomonas fluorescens
3.1.1.1 Tween 40 6.3fold increased activity Pseudomonas fluorescens
3.1.1.1 Tween 60 6.2fold increased activity Pseudomonas fluorescens
3.1.1.1 Tween 80 6.6fold increased activity Pseudomonas fluorescens

Application

EC Number Application Comment Organism
3.1.1.1 biotechnology engineered enzyme can be used to produce the enantiomer (S)-ketoprofen for pharmacological or pharmaceutical use Pseudomonas fluorescens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.1 genetic analysis, expression in Escherichia coli as N-terminally His-tagged enzyme Pseudomonas fluorescens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.1 2-propanol slight inhibition Pseudomonas fluorescens
3.1.1.1 acetone slight inhibition Pseudomonas fluorescens
3.1.1.1 Ag2+ strong inhibition Pseudomonas fluorescens
3.1.1.1 butanol strong inhibition Pseudomonas fluorescens
3.1.1.1 ethanol
-
 Pseudomonas fluorescens
3.1.1.1 Fe2+ strong inhibition Pseudomonas fluorescens
3.1.1.1 Hg2+ strong inhibition Pseudomonas fluorescens
3.1.1.1 additional information no inhibition by methanol Pseudomonas fluorescens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.1 additional information
-
 additional information
-
 Pseudomonas fluorescens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.1 40000 45000 recombinant enzyme, gel filtration Pseudomonas fluorescens
3.1.1.1 42000
-
 1 * 42000, recombinant enzyme, SDS-PAGE Pseudomonas fluorescens

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.1 Pseudomonas fluorescens Q8VU79
-
-
3.1.1.1 Pseudomonas fluorescens KCTC1767 Q8VU79
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.1 recombinant His-tagged enzyme from Escherichia coli Pseudomonas fluorescens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.1 77
-
 purified recombinant enzyme, substrate 4-nitrophenyl palmitate Pseudomonas fluorescens
3.1.1.1 387
-
 purified recombinant enzyme, substrate 4-nitrophenyl caprylate Pseudomonas fluorescens
3.1.1.1 2598
-
 purified recombinant enzyme, substrate 4-nitrophenyl butyrate Pseudomonas fluorescens
3.1.1.1 5242
-
 purified recombinant enzyme, substrate 4-nitrophenyl acetate Pseudomonas fluorescens
3.1.1.1 27000
-
 purified recombinant enzyme, substrate (S)-ketoprofen ethyl ester Pseudomonas fluorescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.1 (S)-ketoprofen ethyl ester + H2O enantioselective activity, best substrate Pseudomonas fluorescens (S)-ketoprofen + ethanol
-
 ?
3.1.1.1 (S)-ketoprofen ethyl ester + H2O enantioselective activity, best substrate Pseudomonas fluorescens KCTC1767 (S)-ketoprofen + ethanol
-
 ?
3.1.1.1 4-nitrophenyl acetate + H2O
-
 Pseudomonas fluorescens 4-nitrophenol + acetate
-
 ?
3.1.1.1 4-nitrophenyl acetate + H2O
-
 Pseudomonas fluorescens KCTC1767 4-nitrophenol + acetate
-
 ?
3.1.1.1 4-nitrophenyl butyrate + H2O
-
 Pseudomonas fluorescens 4-nitrophenol + butyrate
-
 ?
3.1.1.1 4-nitrophenyl butyrate + H2O
-
 Pseudomonas fluorescens KCTC1767 4-nitrophenol + butyrate
-
 ?
3.1.1.1 4-nitrophenyl caprylate + H2O
-
 Pseudomonas fluorescens 4-nitrophenol + caprylate
-
 ?
3.1.1.1 4-nitrophenyl caprylate + H2O
-
 Pseudomonas fluorescens KCTC1767 4-nitrophenol + caprylate
-
 ?
3.1.1.1 4-nitrophenyl palmitate + H2O low activity Pseudomonas fluorescens 4-nitrophenol + palmitate
-
 ?
3.1.1.1 4-nitrophenyl palmitate + H2O low activity Pseudomonas fluorescens KCTC1767 4-nitrophenol + palmitate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.1 monomer 1 * 42000, recombinant enzyme, SDS-PAGE Pseudomonas fluorescens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.1 30
-
-
 Pseudomonas fluorescens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.1 20 35 stable Pseudomonas fluorescens
3.1.1.1 40
-
 2 h, loss of 50% activity Pseudomonas fluorescens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.1 9.5 10
-
 Pseudomonas fluorescens