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Literature summary extracted from
- Expression of a Brassic napus glutamate 1-semialdehyde aminotransferase in Escherichia coli and characterization of the recombinant protein (2003), Protein Expr. Purif., 29, 193-201.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.4.3.8 | glutamate | 3fold activation of tagless recombinant enzyme | Brassica napus |  |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.4.3.8 | expression in Escherichia coli | Brassica napus |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 5.4.3.8 | 45500 | - |
2 * 45500, SDS-PAGE, native and recombinant GSA-AT | Brassica napus |
| 5.4.3.8 | 88000 | - |
native and recombinant GSA-AT, gel filtration | Brassica napus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.3.8 | (S)-4-amino-5-oxopentanoate | Brassica napus | involved in chlorophyll biosynthesis | 5-aminolevulinate | - |
r | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.4.3.8 | Brassica napus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.4.3.8 | recombinant GSA-AT | Brassica napus |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 5.4.3.8 | 0.015 | - |
recombinant GSA-AT | Brassica napus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.3.8 | (S)-4-amino-5-oxopentanoate | - |
Brassica napus | 5-aminolevulinate | - |
r | |
| 5.4.3.8 | (S)-4-amino-5-oxopentanoate | involved in chlorophyll biosynthesis | Brassica napus | 5-aminolevulinate | - |
r | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 5.4.3.8 | dimer | 2 * 45500, SDS-PAGE, native and recombinant GSA-AT | Brassica napus |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 5.4.3.8 | GSA-AT | - |
Brassica napus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.4.3.8 | pyridoxal 5'-phosphate | - |
Brassica napus | |
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Release 2023.1 (January 2023)
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