EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.3.11 | expression in Escherichia coli | Pisum sativum |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.3.11 | AMP | non-competitive inhibition | Pisum sativum | |
3.1.3.11 | D-fructose-2,6-bisphosphate | competitive inhibition | Pisum sativum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.3.11 | 0.0025 | - |
D-fructose 1,6-bisphosphate | pH 6.6, 25°C, native enzyme | Pisum sativum | |
3.1.3.11 | 0.005 | - |
D-fructose 1,6-bisphosphate | pH 6.6, 25°C, Trombin treated enzyme | Pisum sativum | |
3.1.3.11 | 0.01 | - |
D-fructose 1,6-bisphosphate | pH 6.6, 25°C, recombinant enzyme | Pisum sativum |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.1.3.11 | chloroplast | - |
Pisum sativum | 9507 | - |
3.1.3.11 | cytoplasm | - |
Pisum sativum | 5737 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.3.11 | 38000 | - |
- |
Pisum sativum |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.3.11 | D-fructose 1,6-bisphosphate + H2O | Pisum sativum | the the chloroplastic isozyme plays a key role in the Calvin cycle and the cytoplasmic isozyme plays a key role in the sucrose synthesis in cytoplasm | D-fructose 6-phosphate + phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.3.11 | Escherichia coli | - |
- |
- |
3.1.3.11 | Pisum sativum | Q8RW99 | - |
- |
3.1.3.11 | Spinacia oleracea | - |
- |
- |
3.1.3.11 | Sus scrofa | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.3.11 | the recombinant enzyme | Pisum sativum |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.1.3.11 | 8.16 | - |
- |
Pisum sativum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.3.11 | D-fructose 1,6-bisphosphate + H2O | - |
Pisum sativum | D-fructose 6-phosphate + phosphate | - |
? | |
3.1.3.11 | D-fructose 1,6-bisphosphate + H2O | the the chloroplastic isozyme plays a key role in the Calvin cycle and the cytoplasmic isozyme plays a key role in the sucrose synthesis in cytoplasm | Pisum sativum | D-fructose 6-phosphate + phosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.3.11 | monomer | 1 * 38000, SDS-PAGE | Pisum sativum |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.3.11 | 55 | - |
optimum of recombinant enzyme | Pisum sativum |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.3.11 | 45 | - |
the native enzyme is completely inactivated at temperatures higher than 45°C | Pisum sativum |
3.1.3.11 | 55 | - |
the activity of recombinant enzyme is increased at temperatures higher than 55°C | Pisum sativum |
3.1.3.11 | 65 | - |
the recombinant enzyme is completely inactivated at temperatures higher than 65°C | Pisum sativum |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.3.11 | 7 | - |
the recombinant enzyme | Pisum sativum |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.1.3.11 | 6.5 | 9 | the recombinant enzyme | Pisum sativum |
3.1.3.11 | 7.5 | 8.2 | the native enzyme | Pisum sativum |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.3.11 | 0.00007 | - |
D-fructose 2,6-bisphosphate | pH 6.6, 25°C, native enzyme | Pisum sativum | |
3.1.3.11 | 0.0001 | - |
D-fructose 2,6-bisphosphate | pH 6.6, 25°C, Trombin treated enzyme | Pisum sativum | |
3.1.3.11 | 0.00015 | - |
D-fructose 2,6-bisphosphate | pH 6.6, 25°C, recombinant enzyme | Pisum sativum | |
3.1.3.11 | 0.12 | - |
AMP | pH 6.6, 25°C, native enzyme | Pisum sativum | |
3.1.3.11 | 0.16 | - |
AMP | pH 6.6, 25°C, recombinant enzyme | Pisum sativum | |
3.1.3.11 | 0.16 | - |
AMP | pH 6.6, 25°C, Trombin treated enzyme | Pisum sativum |