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Literature summary extracted from

  • Quyen, D.T.; Schmidt-Dannert, C.; Schmid, R.D.
    High-level expression of a lipase from Bacillus thermocatenulatus BTL2 in Pichia pastoris and some properties of the recombinant lipase (2003), Protein Expr. Purif., 28, 102-110.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.3 amino acid determinatioin of the purified enzyme, high-level expression in Pichia pastoris GS115, subcloning in Escherichia coli DH5alpha Geobacillus thermocatenulatus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.3 2-propanol
-
 Geobacillus thermocatenulatus
3.1.1.3 acetone
-
 Geobacillus thermocatenulatus
3.1.1.3 cholate 1% w/v, 20% decrease in activity Geobacillus thermocatenulatus
3.1.1.3 methanol
-
 Geobacillus thermocatenulatus
3.1.1.3 SDS 1% w/v, 95% decrease in activity Geobacillus thermocatenulatus
3.1.1.3 Triton X-100 1% w/v, 30% decrease in activity Geobacillus thermocatenulatus
3.1.1.3 Tween 20 1% w/v, 7% decrease in activity Geobacillus thermocatenulatus
3.1.1.3 Tween 80 1% w/v, 6% decrease in activity Geobacillus thermocatenulatus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.3 43000
-
 x * 43000, recombinant enzyme, SDS-PAGE Geobacillus thermocatenulatus

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.1.1.3 2-propanol 30% v/v, 1 h, 30°C, 0.1 M Tris buffer, pH 8.0, 47% reduced activity Geobacillus thermocatenulatus
3.1.1.3 Acetone 30% v/v, 1 h, 30°C, 0.1 M Tris buffer, pH 8.0, 16% reduced activity Geobacillus thermocatenulatus
3.1.1.3 Methanol 30% v/v, 1 h, 30°C, 0.1 M Tris buffer, pH 8.0, 16% reduced activity Geobacillus thermocatenulatus

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.3 Geobacillus thermocatenulatus
-
-
-
3.1.1.3 Geobacillus thermocatenulatus BTL2
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.3 large scale from Pichia pastoris culture supernatant, 660fold Geobacillus thermocatenulatus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.3 23000
-
 purified enzyme, substrate tributyrin Geobacillus thermocatenulatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.3 4-nitrophenyl palmitate + H2O
-
 Geobacillus thermocatenulatus 4-nitrophenol + palmitate
-
 ?
3.1.1.3 4-nitrophenyl palmitate + H2O
-
 Geobacillus thermocatenulatus BTL2 4-nitrophenol + palmitate
-
 ?
3.1.1.3 triacetin + H2O poor substrate Geobacillus thermocatenulatus diacetin + acetate
-
 ?
3.1.1.3 tributyrin + H2O best substrate Geobacillus thermocatenulatus dibutyrin + butyrate
-
 ?
3.1.1.3 tributyrin + H2O best substrate Geobacillus thermocatenulatus BTL2 dibutyrin + butyrate
-
 ?
3.1.1.3 tricaprin + H2O about 20% of the activity with tributyrin Geobacillus thermocatenulatus dicaprin + decaprate
-
 ?
3.1.1.3 tricaproin + H2O about 20% of the activity with tributyrin Geobacillus thermocatenulatus dicaproin + caproate
-
 ?
3.1.1.3 trilaurin + H2O about 10% of the activity with tributyrin Geobacillus thermocatenulatus dilaurin + laurate
-
 ?
3.1.1.3 trilaurin + H2O about 10% of the activity with tributyrin Geobacillus thermocatenulatus BTL2 dilaurin + laurate
-
 ?
3.1.1.3 trimyristin + H2O about 10% of the activity with tributyrin Geobacillus thermocatenulatus dimyristin + myristate
-
 ?
3.1.1.3 triolein + H2O
-
 Geobacillus thermocatenulatus diolein + oleate
-
 ?
3.1.1.3 triolein + H2O
-
 Geobacillus thermocatenulatus BTL2 diolein + oleate
-
 ?
3.1.1.3 tripalmitin + H2O about 20% of the activity with tributyrin Geobacillus thermocatenulatus dipalmitin + palmitate
-
 ?
3.1.1.3 tripalmitin + H2O about 20% of the activity with tributyrin Geobacillus thermocatenulatus BTL2 dipalmitin + palmitate
-
 ?
3.1.1.3 tristearin + H2O about 10% of the activity with tributyrin Geobacillus thermocatenulatus distearin + stearate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.3 ? x * 43000, recombinant enzyme, SDS-PAGE Geobacillus thermocatenulatus

Synonyms

EC Number Synonyms Comment Organism
3.1.1.3 lipase
-
 Geobacillus thermocatenulatus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.3 65
-
 with substrate tributyrin, pH 8.0 Geobacillus thermocatenulatus
3.1.1.3 75
-
 with substrate triolein, pH 8.0 Geobacillus thermocatenulatus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.3 35 80
-
 Geobacillus thermocatenulatus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.3 50
-
 stable up to, pH 8.0, 0.1 M Tris buffer Geobacillus thermocatenulatus
3.1.1.3 70
-
 18% remaining activity Geobacillus thermocatenulatus
3.1.1.3 80
-
 5% remaining activity Geobacillus thermocatenulatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.3 7.5
-
 with substrate tributyrin, 60°C Geobacillus thermocatenulatus
3.1.1.3 9
-
 with substrate triolein, 60°C Geobacillus thermocatenulatus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.3 5 9
-
 Geobacillus thermocatenulatus