BRENDA - Enzyme Database show

Purification and characterization of recombinant microsomal prostaglandin E synthase-1

Ouellet, M.; Falgueyret, J.P.; Ear, P.H.; Pen, A.; Mancini, J.A.; Riendeau, D.; David Percival, M.; Protein Expr. Purif. 26, 489-495 (2002)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
5.3.99.3
captoprylsulfobetaine
10 mM, 2.6fold activation
Homo sapiens
5.3.99.3
diheptanoylphosphatidylcholine
2.2 mM, 4.8fold activation
Homo sapiens
5.3.99.3
n-dodecylmaltoside
0.3 mM, 5fold activation
Homo sapiens
5.3.99.3
n-octylglucoside
34 mM, 3.3fold activation
Homo sapiens
5.3.99.3
Triton X-100
5 mM, 4.6fold activation
Homo sapiens
Cloned(Commentary)
EC Number
Commentary
Organism
5.3.99.3
expression in Sf9 insect cells
Homo sapiens
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
5.3.99.3
MK-0886
0.002 mM, 50% inhibition
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.3.99.3
0.014
-
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate
-
Homo sapiens
5.3.99.3
0.1
-
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate
in the presence of 0.3 mM dodecylmaltoside
Homo sapiens
5.3.99.3
0.6
-
glutathione
in the presence of 0.3 mM dodecylmaltoside
Homo sapiens
5.3.99.3
0.75
-
glutathione
-
Homo sapiens
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
5.3.99.3
integral to membrane
-
Homo sapiens
-
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
5.3.99.3
17000
-
x * 17000, SDS-PAGE
Homo sapiens
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
5.3.99.3
Homo sapiens
O14684
-
-
Purification (Commentary)
EC Number
Commentary
Organism
5.3.99.3
detergent, hydroxyapatite column
Homo sapiens
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
5.3.99.3
4
-
-
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.3.99.3
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate + reduced glutathione
i.e. prostaglandin H2
653753
Homo sapiens
(5Z,13E)-(15S)-11alpha,15-dihydroxyprosta-5,13-dienoate + oxidized glutathione
i.e. prostaglandin E2
653753
Homo sapiens
?
Subunits
EC Number
Subunits
Commentary
Organism
5.3.99.3
?
x * 17000, SDS-PAGE
Homo sapiens
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
5.3.99.3
7
-
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate
dodecylmaltoside activated
Homo sapiens
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
5.3.99.3
glutathione
-
Homo sapiens
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
5.3.99.3
captoprylsulfobetaine
10 mM, 2.6fold activation
Homo sapiens
5.3.99.3
diheptanoylphosphatidylcholine
2.2 mM, 4.8fold activation
Homo sapiens
5.3.99.3
n-dodecylmaltoside
0.3 mM, 5fold activation
Homo sapiens
5.3.99.3
n-octylglucoside
34 mM, 3.3fold activation
Homo sapiens
5.3.99.3
Triton X-100
5 mM, 4.6fold activation
Homo sapiens
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
5.3.99.3
expression in Sf9 insect cells
Homo sapiens
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
5.3.99.3
glutathione
-
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
5.3.99.3
MK-0886
0.002 mM, 50% inhibition
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.3.99.3
0.014
-
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate
-
Homo sapiens
5.3.99.3
0.1
-
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate
in the presence of 0.3 mM dodecylmaltoside
Homo sapiens
5.3.99.3
0.6
-
glutathione
in the presence of 0.3 mM dodecylmaltoside
Homo sapiens
5.3.99.3
0.75
-
glutathione
-
Homo sapiens
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
5.3.99.3
integral to membrane
-
Homo sapiens
-
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
5.3.99.3
17000
-
x * 17000, SDS-PAGE
Homo sapiens
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
5.3.99.3
detergent, hydroxyapatite column
Homo sapiens
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
5.3.99.3
4
-
-
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.3.99.3
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate + reduced glutathione
i.e. prostaglandin H2
653753
Homo sapiens
(5Z,13E)-(15S)-11alpha,15-dihydroxyprosta-5,13-dienoate + oxidized glutathione
i.e. prostaglandin E2
653753
Homo sapiens
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
5.3.99.3
?
x * 17000, SDS-PAGE
Homo sapiens
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
5.3.99.3
7
-
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate
dodecylmaltoside activated
Homo sapiens