Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Hellberg, A.; Nowak, N.; Leippe, M.; Tannich, E.; Bruchhaus, I.
    Recombinant expression and purification of an enzymatically active cysteine proteinase of the protozoan parasite Entamoeba histolytica (2002), Protein Expr. Purif., 24, 131-137.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.22.35 expression of the His-tagged inactive pro-enzyme in Escherichia coli BL21(DE3) Entamoeba histolytica

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.35 antipain
-
 Entamoeba histolytica
3.4.22.35 iodoacetamide
-
 Entamoeba histolytica
3.4.22.35 leupeptin
-
 Entamoeba histolytica
3.4.22.35 trans-epoxysuccinyl-L-leucylamido-(4-guanidino)butane ie.e. E-64, irreversible Entamoeba histolytica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.22.35 protein + H2O Entamoeba histolytica
-
 peptides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.35 Entamoeba histolytica
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.22.35 additional information processing of the inactive pro-enzyme to the mature active form by treatment with 10 mM DTT and 0.04% SDS at 37°C and pH 8.8, SDS can be substituted by Triton X-100 and octylglucoside but these are 3fold less efficient Entamoeba histolytica

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.35 recombinant His-tagged inactive pro-enzyme to homogeneity Entamoeba histolytica

Renatured (Commentary)

EC Number Renatured (Comment) Organism
3.4.22.35 renaturation of purified inactive pro-enzyme by a 100fold dilution in a buffer containing reduced and oxidized thiols leading to a soluble inactive pro-enzyme Entamoeba histolytica

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.22.35 170
-
 purified native enzyme, substrate azocasein Entamoeba histolytica
3.4.22.35 180
-
 purified recombinant enzyme, substrate azocasein Entamoeba histolytica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.35 azocasein + H2O
-
 Entamoeba histolytica fragments of azocasein
-
 ?
3.4.22.35 benzyloxycarbonyl-Ala-Arg-Arg-4-nitroanilide + H2O
-
 Entamoeba histolytica benzyloxycarbonyl-Ala-Arg-Arg + 4-nitroaniline
-
 ?
3.4.22.35 benzyloxycarbonyl-Arg-Arg-4-nitroanilide + H2O
-
 Entamoeba histolytica benzyloxycarbonyl-Arg-Arg + 4-nitroaniline
-
 ?
3.4.22.35 Bovine serum albumin + H2O
-
 Entamoeba histolytica ?
-
 ?
3.4.22.35 Collagen + H2O
-
 Entamoeba histolytica ?
-
 ?
3.4.22.35 Fibronectin + H2O
-
 Entamoeba histolytica ?
-
 ?
3.4.22.35 Gelatin + H2O
-
 Entamoeba histolytica ?
-
 ?
3.4.22.35 Hemoglobin + H2O
-
 Entamoeba histolytica ?
-
 ?
3.4.22.35 immunoglobulin G + H2O
-
 Entamoeba histolytica ?
-
 ?
3.4.22.35 additional information no activity with benzyloxycarbonyl-Phe-Arg-4-nitroanilide Entamoeba histolytica ?
-
 ?
3.4.22.35 mucin + H2O
-
 Entamoeba histolytica ?
-
 ?
3.4.22.35 protein + H2O
-
 Entamoeba histolytica peptides
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.22.35 ? x * 27000-29000, recombinant and native enzyme, reducing and nonreducing SDS-PAGE Entamoeba histolytica

Synonyms

EC Number Synonyms Comment Organism
3.4.22.35 EhCP5
-
 Entamoeba histolytica
3.4.22.35 Entamoeba histolytica cysteine proteinase 5
-
 Entamoeba histolytica

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.22.35 37
-
 assay at Entamoeba histolytica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.35 5.5 8 recombinant enzyme Entamoeba histolytica

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.22.35 4.5 9.5 recombinant enzyme Entamoeba histolytica