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Literature summary extracted from
- N-terminal portion acts as an initiator of the inactivation of pepsin at neutral pH (2001), Protein Eng., 14, 669-674.
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.4.23.1 | DELTA240-246/+GD | inactivation rate at pH 7.0 is 86% of that of the wild-type value. The Km-value for KPAEFF(NO2)AL is 2fold higher than that of the wild-type enzyme. The KM-value for LSF(NO2)-Nle-AL as substrate is 79% of the wild-type value | Sus scrofa |
| 3.4.23.1 | G2C/L167C | the mutation reduces the inactivation rate at pH 7.0 by 1.8times compared to wild-type value. The Km-value for KPAEFF(NO2)AL is 2.35fold higher than that of the wild-type enzyme. The KM-value for LSF(NO2)-Nle-AL as substrate is 95% of the wild-type value | Sus scrofa |
| 3.4.23.1 | G2S/D3Y | the mutation reduces the inactivation rate at pH 7.0 by 1.4times compared to wild-type value. The Km-value for KPAEFF(NO2)AL is 2.2fold higher than that of the wild-type enzyme. The KM-value for LSF(NO2)-Nle-AL as substrate is 1.5fold higher than that of the wild-type enzyme | Sus scrofa |
| 3.4.23.1 | G2S/D3Y/L10M/T12A/E13S | the mutation reduces the inactivation rate at pH 7.0 by 5.8times. In presence of glycerol and sucrose, this mutant shows a very low rate of inactivation, the residual activity after 240 min is 50% of the initial activity compared to the wild-type which loses most of its activity in 60 min. The Km-value for KPAEFF(NO2)AL is 2.4fold higher than that of the wild-type enzyme. The KM-value for LSF(NO2)-Nle-AL as substrate is 1.5fold higher than that of the wild-type enzyme | Sus scrofa |
| 3.4.23.1 | L10M/T12A/E13S | the mutation reduces the inactivation rate at pH 7.0 by 1.5times compared to wild-type value. The Km-value for KPAEFF(NO2)AL is 1.9fold higher than that of the wild-type enzyme. The KM-value for LSF(NO2)-Nle-AL as substrate is 2.2fold higher than that of the wild-type enzyme | Sus scrofa |
| 3.4.23.1 | S196R/D200G/E202K | inactivation rate at pH 7.0 is 86% of that of the wild-type value | Sus scrofa |
| 3.4.23.1 | S46K/D52N/N54K/Q55R/D60K | inactivation rate at pH 7.0 is 91% of that of the wild-type value | Sus scrofa |
| 3.4.23.1 | S46K/D52N/N54K/Q55R/D60K/S196R/D200G/E202K | the mutation reduces the inactivation rate at pH 7.0 by 2.3times compared to wild-type value. Km-value for KPAEFF(NO2)AL is 1.7fold higher than that of the wild-type enzyme. The KM-value for LSF(NO2)-Nle-AL as substrate is 1.6fold higher than that of the wild-type enzyme | Sus scrofa |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.23.1 | 0.015 | - |
LSF(NO2)-Nle-AL | pH 3.95, 37°C, mutant enzyme DELTA240-246/+GD | Sus scrofa |  |
| 3.4.23.1 | 0.018 | - |
LSF(NO2)-Nle-AL | pH 3.95, 37°C, mutant enzyme G2C/L167C | Sus scrofa | |
| 3.4.23.1 | 0.019 | - |
LSF(NO2)-Nle-AL | pH 3.95, 37°C, wild-type enzyme | Sus scrofa | |
| 3.4.23.1 | 0.028 | - |
LSF(NO2)-Nle-AL | pH 3.95, 37°C, mutant enzyme G2S/D3Y | Sus scrofa | |
| 3.4.23.1 | 0.028 | - |
LSF(NO2)-Nle-AL | pH 3.95, 37°C, mutant enzyme G2S/D3Y/L10M/T12A/E13S | Sus scrofa | |
| 3.4.23.1 | 0.031 | - |
LSF(NO2)-Nle-AL | pH 3.95, 37°C, mutant enzyme S46K/D52N/N54K/Q55R/D60K/S196R/D200G/E202K | Sus scrofa | |
| 3.4.23.1 | 0.034 | - |
KPAEFF(NO2)AL | pH 3.95, 37°C, wild-type enzyme | Sus scrofa | |
| 3.4.23.1 | 0.041 | - |
LSF(NO2)-Nle-AL | pH 3.95, 37°C, mutant enzyme L10M/T12A/E13S | Sus scrofa | |
| 3.4.23.1 | 0.057 | - |
KPAEFF(NO2)AL | pH 3.95, 37°C, mutant enzyme S46K/D52N/N54K/Q55R/D60K/S196R/D200G/E202K | Sus scrofa | |
| 3.4.23.1 | 0.065 | - |
KPAEFF(NO2)AL | pH 3.95, 37°C, mutant enzyme L10M/T12A/E13S | Sus scrofa | |
| 3.4.23.1 | 0.07 | - |
KPAEFF(NO2)AL | pH 3.95, 37°C, mutant enzyme DELTA240-246/+GD | Sus scrofa | |
| 3.4.23.1 | 0.075 | - |
KPAEFF(NO2)AL | pH 3.95, 37°C, mutant enzyme G2S/D3Y | Sus scrofa | |
| 3.4.23.1 | 0.08 | - |
KPAEFF(NO2)AL | pH 3.95, 37°C, mutant enzyme G2C/L167C | Sus scrofa | |
| 3.4.23.1 | 0.083 | - |
KPAEFF(NO2)AL | pH 3.95, 37°C, mutant enzyme G2S/D3Y/L10M/T12A/E13S | Sus scrofa | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.23.1 | Sus scrofa | - |
- |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.23.1 | KPAEFF(NO2)AL + H2O | - |
Sus scrofa | ? | - |
? | |
| 3.4.23.1 | LSF(NO2)-Nle-AL + H2O | - |
Sus scrofa | ? | - |
? | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.23.1 | 2.1 | - |
- |
Sus scrofa |
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