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Literature summary extracted from

  • Sharma, R.; Soni, S.K.; Vohra, R.M.; Gupta, L.K.; Gupta, J.K.
    Purification and characterization of a thermostable alkaline lipase from a new thermophilic Bacillus sp. RSJ-1 (2002), Process Biochem., 37, 1075-1084.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.3 additional information not affected by EDTA Bacillus sp. (in: Bacteria)

Application

EC Number Application Comment Organism
3.1.1.3 detergent the enzyme is usable with laundry detergents Bacillus sp. (in: Bacteria)

General Stability

EC Number General Stability Organism
3.1.1.3 Ca2+ stabilizes the enzyme at concentration of 1-10 mM with decreasing effect at higher concentration, 100% activity at 1 mM, 4% activity at 10 mM Bacillus sp. (in: Bacteria)

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.3 2-mercaptoethanol
-
 Bacillus sp. (in: Bacteria)
3.1.1.3 ammonium persulfate
-
 Bacillus sp. (in: Bacteria)
3.1.1.3 ascorbic acid
-
 Bacillus sp. (in: Bacteria)
3.1.1.3 Co2+
-
 Bacillus sp. (in: Bacteria)
3.1.1.3 Cs+
-
 Bacillus sp. (in: Bacteria)
3.1.1.3 Cu2+
-
 Bacillus sp. (in: Bacteria)
3.1.1.3 deoxycholate
-
 Bacillus sp. (in: Bacteria)
3.1.1.3 K+
-
 Bacillus sp. (in: Bacteria)
3.1.1.3 Mn2+
-
 Bacillus sp. (in: Bacteria)
3.1.1.3 additional information not affected by EDTA Bacillus sp. (in: Bacteria)
3.1.1.3 Ni2+ slight inhibition Bacillus sp. (in: Bacteria)
3.1.1.3 potassium iodide
-
 Bacillus sp. (in: Bacteria)
3.1.1.3 Sn2+
-
 Bacillus sp. (in: Bacteria)
3.1.1.3 Tween-20
-
 Bacillus sp. (in: Bacteria)
3.1.1.3 Zn2+
-
 Bacillus sp. (in: Bacteria)

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.3 2.2
-
 4-nitrophenyl butyrate pH 8.0, 50°C Bacillus sp. (in: Bacteria)

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.3 Ca2+
-
 Bacillus sp. (in: Bacteria)
3.1.1.3 additional information the enzyme is no metalloenzyme, not affected by citrate, Ba2+, K+, and Mg2+ Bacillus sp. (in: Bacteria)
3.1.1.3 Na+ slight activation Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.3 37000
-
 x * 37000, SDS-PAGE Bacillus sp. (in: Bacteria)

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.3 Bacillus sp. (in: Bacteria)
-
 thermostable alkaline lipase
-
3.1.1.3 Bacillus sp. (in: Bacteria) RSJ-1
-
 thermostable alkaline lipase
-

Oxidation Stability

EC Number Oxidation Stability Organism
3.1.1.3 enzyme retains 93% activity at 0.1% H2O2, 74% activity at 0.2% H2O2, and 47% activity at 1% H2O2 Bacillus sp. (in: Bacteria)

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.3 to homogeneity from culture supernatant, 201fold Bacillus sp. (in: Bacteria)

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.3 428.1
-
 purified enzyme, substrate 4-nitrophenyl butyrate Bacillus sp. (in: Bacteria)

Storage Stability

EC Number Storage Stability Organism
3.1.1.3 enzyme is stable in nonionic detergents such as Triton X-100, Tween-80, and Tween-20, but is less stable in presence of anionic detergents e.g. deoxycholate Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.3 4-nitrophenyl butyrate + H2O
-
 Bacillus sp. (in: Bacteria) 4-nitrophenol + butyrate
-
 ?
3.1.1.3 4-nitrophenyl butyrate + H2O
-
 Bacillus sp. (in: Bacteria) RSJ-1 4-nitrophenol + butyrate
-
 ?
3.1.1.3 triolein + H2O
-
 Bacillus sp. (in: Bacteria) diolein + oleate
-
 ?
3.1.1.3 triolein + H2O
-
 Bacillus sp. (in: Bacteria) RSJ-1 diolein + oleate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.3 ? x * 37000, SDS-PAGE Bacillus sp. (in: Bacteria)

Synonyms

EC Number Synonyms Comment Organism
3.1.1.3 lipase
-
 Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.3 50
-
-
 Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.3 50
-
 stable for 60 min, after 120 min 90% remaining activity Bacillus sp. (in: Bacteria)
3.1.1.3 55
-
 half-life 240 min Bacillus sp. (in: Bacteria)
3.1.1.3 60
-
 half-life 150 min Bacillus sp. (in: Bacteria)
3.1.1.3 65
-
 half-life 90 min Bacillus sp. (in: Bacteria)
3.1.1.3 70
-
 half-life 45 min Bacillus sp. (in: Bacteria)
3.1.1.3 75
-
 half-life 30 min Bacillus sp. (in: Bacteria)

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.3 8
-
-
 Bacillus sp. (in: Bacteria)

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.3 5 12 12% of maximal activity at pH 5.0, 18% of maximal activity at pH 12.0 Bacillus sp. (in: Bacteria)