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Literature summary extracted from

  • Zhang, D.; Vaidehi, N.; Goddard, W.A.3rd.; Danzer, J.F.; Debe, D.
    Structure-based design of mutant Methanococcus jannaschii tyrosyl-tRNA synthetase for incorporation of O-methyl-L-tyrosine (2002), Proc. Natl. Acad. Sci. USA, 99, 6579-6584.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
6.1.1.1 biotechnology incorporation of unusual specific amino acids into proteins in in vitro translation systems by mutant enzyme with altered substrate specificity. e.g. mutant Y32Q/D158A Methanocaldococcus jannaschii

Protein Variants

EC Number Protein Variants Comment Organism
6.1.1.1 additional information computational design of possible mutants with altered substrate specificity by prediction of the binding structure and calculation of binding energies of mutants to amino acids, overview Methanocaldococcus jannaschii
6.1.1.1 Y32Q/D158A site-directed mutagenesis, enzyme mutant discriminates between L-tyrosine and O-methyl-L-tyrosine, with a high activity only with the latter Methanocaldococcus jannaschii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.1 ATP + L-tyrosine + tRNATyr Methanocaldococcus jannaschii
-
 AMP + L-Tyr-tRNATyr + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.1 Methanocaldococcus jannaschii Q57834
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.1 ATP + L-tyrosine + tRNATyr
-
 Methanocaldococcus jannaschii AMP + L-Tyr-tRNATyr + diphosphate
-
 ?
6.1.1.1 ATP + O-methyl-L-tyrosine + tRNATyr
-
 Methanocaldococcus jannaschii AMP + O-methyl-L-Tyr-tRNATyr + diphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.1.1.1 Tyrosyl-tRNA synthetase
-
 Methanocaldococcus jannaschii
6.1.1.1 TyrRS
-
 Methanocaldococcus jannaschii