Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Stathopoulos, C.; Kim, W.; Li, T.; Anderson, I.; Deutsch, B.; Palioura, S.; Whitman, W.; Soll, D.
    Cysteinyl-tRNA synthetase is not essential for viability of the archaeon Methanococcus maripaludis (2001), Proc. Natl. Acad. Sci. USA, 98, 14292-14297.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.15 expression in Escherichia coli Methanococcus maripaludis
6.1.1.16 overexpression of the wild-type enzyme in Escherichia coli BL21(DE3) as N-terminally HIs-tagged protein Methanococcus maripaludis

Protein Variants

EC Number Protein Variants Comment Organism
6.1.1.16 additional information construction of a cysS gene disruption knockout mutant, which is viable under normal growth conditions Methanococcus maripaludis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.15 0.0046
-
 L-proline pH 7.0, 37°C Methanococcus maripaludis
6.1.1.15 0.0745
-
 L-cysteine pH 7.0, 37°C Methanococcus maripaludis
6.1.1.16 0.0097
-
 L-cysteine recombinant wild-type enzyme, pH 7.0, 37°C Methanococcus maripaludis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.15 ATP + L-proline + tRNAPro Methanococcus maripaludis
-
 AMP + diphosphate + L-prolyl-tRNAPro
-
 ?
6.1.1.16 ATP + L-cysteine + tRNACys Methanococcus maripaludis the canonical enzyme is not essential for viability of the archaeon Methanococcus maripaludis probably due to the dual-specific ProCys-tRNA synthetase AMP + diphosphate + L-cysteinyl-tRNACys
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.15 Methanococcus maripaludis Q6LZD3
-
-
6.1.1.16 Methanococcus maripaludis Q9UWP2 gene cysS, canonical enzyme form CysRS
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.15
-
 Methanococcus maripaludis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.15 ATP + L-cysteine + tRNA in later work it is shown that cysteine is attached to tRNA(Pro) and thus constitutes a misaminoacylation event and not a dual specificity Methanococcus maripaludis AMP + diphosphate + L-cysteinyl-tRNA
-
 ?
6.1.1.15 ATP + L-proline + tRNAPro
-
 Methanococcus maripaludis AMP + diphosphate + L-prolyl-tRNAPro
-
 ?
6.1.1.16 ATP + L-cysteine + tRNACys
-
 Methanococcus maripaludis AMP + diphosphate + L-cysteinyl-tRNACys
-
 ?
6.1.1.16 ATP + L-cysteine + tRNACys the canonical enzyme is not essential for viability of the archaeon Methanococcus maripaludis probably due to the dual-specific ProCys-tRNA synthetase Methanococcus maripaludis AMP + diphosphate + L-cysteinyl-tRNACys
-
 ?
6.1.1.16 additional information the enzyme also performs the ATP-diphosphate exchange reaction Methanococcus maripaludis ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.1.1.15 dual-specificity prolyl-tRNA synthetase misleading Methanococcus maripaludis
6.1.1.15 ProCysRS
-
 Methanococcus maripaludis
6.1.1.16 CysRS
-
 Methanococcus maripaludis
6.1.1.16 Cysteine translase
-
 Methanococcus maripaludis
6.1.1.16 Cysteine--tRNA ligase
-
 Methanococcus maripaludis
6.1.1.16 Cysteinyl-transfer ribonucleate synthetase
-
 Methanococcus maripaludis
6.1.1.16 Cysteinyl-transfer RNA synthetase
-
 Methanococcus maripaludis
6.1.1.16 Cysteinyl-tRNA synthetase
-
 Methanococcus maripaludis
6.1.1.16 Synthetase, cysteinyl-transfer ribonucleate
-
 Methanococcus maripaludis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.1.1.15 37
-
 assay at Methanococcus maripaludis
6.1.1.16 37
-
 assay at Methanococcus maripaludis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.1.1.15 0.8
-
 L-cysteine pH 7.0, 37°C Methanococcus maripaludis
6.1.1.15 0.9
-
 L-proline pH 7.0, 37°C Methanococcus maripaludis
6.1.1.16 2.2
-
 L-cysteine recombinant wild-type enzyme, pH 7.0, 37°C Methanococcus maripaludis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.1.1.15 7
-
 assay at Methanococcus maripaludis
6.1.1.16 7
-
 assay at Methanococcus maripaludis

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.16 ATP
-
 Methanococcus maripaludis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
6.1.1.15 10.7
-
 L-cysteine pH 7.0, 37°C Methanococcus maripaludis
6.1.1.15 196
-
 L-proline pH 7.0, 37°C Methanococcus maripaludis