EC Number | Cloned (Comment) | Organism |
---|---|---|
6.1.1.19 | overexpression in Escherichia coli of wild type and several mutant enzymes | Thermus thermophilus |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
6.1.1.19 | crystal structure solved by multiple isomorphous replacement | Thermus thermophilus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
6.1.1.19 | N79D | can use tRNAArg with G20 as substrate | Thermus thermophilus |
6.1.1.19 | N79E | can use tRNAArg with G20 or U20 as substrate | Thermus thermophilus |
6.1.1.19 | N79K | can use tRNAArg with G20 as substrate | Thermus thermophilus |
6.1.1.19 | N79Q | can use tRNAArg with G20 as substrate | Thermus thermophilus |
6.1.1.19 | N79R | can use tRNAArg with G20 or U20 as substrate | Thermus thermophilus |
6.1.1.19 | Y77A | inactive mutant | Thermus thermophilus |
6.1.1.19 | Y77F | slight effect on activity | Thermus thermophilus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.1.1.19 | additional information | - |
additional information | Km values for the wild type and mutant enzymes using tRNAArg with different nucleotides at position 20 | Thermus thermophilus | |
6.1.1.19 | 0.0098 | - |
tRNAArg | adenine at position 20 of the tRNAArg is required for activity, pH 7.5, 65°C | Thermus thermophilus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.1.1.19 | Thermus thermophilus | Q93RP5 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.1.1.19 | ATP + L-arginine + tRNAArg | - |
Thermus thermophilus | AMP + diphosphate + L-arginyl-tRNAArg | - |
? |