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Literature summary extracted from

  • Schneider, K.; Hovel, K.; Witzel, K.; Hamberger, B.; Schomburg, D.; Kombrink, E.; Stuible, H.P.
    The substrate specificity-determining amino acid code of 4-coumarate:CoA ligase (2003), Proc. Natl. Acad. Sci. USA, 100, 8601-8606.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.2.1.12
-
 Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
6.2.1.12 L356A only little effect on activity Arabidopsis thaliana
6.2.1.12 M293P/K320L effectively uses ferulate as substrate, changes in Km for all substrates Arabidopsis thaliana
6.2.1.12 additional information domain exchange experiments with gramicidine synthetase from Bacillus brevis Arabidopsis thaliana
6.2.1.12 N256A/M293P/K320L effectively uses ferulate and cinnamate as substrates, 10fold reduced activity with caffeate Arabidopsis thaliana
6.2.1.12 V355A strongly reduced activity Arabidopsis thaliana
6.2.1.12 V355A/L356A strongly reduced activity Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.2.1.12 0.022
-
 caffeate wild-type enzyme Arabidopsis thaliana
6.2.1.12 0.022
-
 4-coumarate M293P/K320L mutant enzyme Arabidopsis thaliana
6.2.1.12 0.03
-
 ferulate M293P/K320L mutant enzyme Arabidopsis thaliana
6.2.1.12 0.041
-
 caffeate M293P/K320L mutant enzyme Arabidopsis thaliana
6.2.1.12 0.233
-
 4-coumarate wild-type enzyme Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.2.1.12 ATP + 4-coumarate + CoA Arabidopsis thaliana plant phenylpropanoid metabolism AMP + diphosphate + 4-coumaroyl-CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.12 Arabidopsis thaliana
-
 isoform 4CL2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.2.1.12 recombinant enzyme Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.12 ATP + 4-coumarate + CoA
-
 Arabidopsis thaliana AMP + diphosphate + 4-coumaroyl-CoA
-
 ?
6.2.1.12 ATP + 4-coumarate + CoA plant phenylpropanoid metabolism Arabidopsis thaliana AMP + diphosphate + 4-coumaroyl-CoA
-
 ?
6.2.1.12 ATP + caffeate + CoA
-
 Arabidopsis thaliana AMP + diphosphate + caffeoyl-CoA
-
 ?
6.2.1.12 ATP + ferulate + CoA very poor substrate for the wild type enzyme, effective substrate for M293P/K320L mutant enzyme Arabidopsis thaliana AMP + diphosphate + feruloyl-CoA
-
 ?