Literature summary extracted from

Kamtekar, S.; Kennedy, W.D.; Wang, J.; Stathopoulos, C.; Soll, D.; Steitz, T.A.
The structural basis of cysteine aminoacylation of tRNAPro by prolyl-tRNA synthetases (2003), Proc. Natl. Acad. Sci. USA, 100, 1673-1678.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
6.1.1.15	expression as His-tagged enzyme in Escherichia coli	Methanothermobacter thermautotrophicus

EC Number	Crystallization (Comment)	Organism
6.1.1.15	vapour diffusion method, equal volumes of 3.8 mg/ml protein solution and precipitant solution containing 20 mM Tris, pH 7.5, 5 mM 2-mercaptoethanol, 50-250 mM MgCl2, 20°C, X-ray diffraction structure determination at 2.55-3.1 A, and analysis	Methanothermobacter thermautotrophicus
6.1.1.15	vapour-diffusion method, protein solution is mixed with an equal volume of well solution containing 0.2 M ammonium sulfate, 20-30% PEG 4000, 5 mM 2-mercaptoethanol, 0.1 M sodium acetate, pH 4.5, 12-25°C, a few days, X-ray diffraction structure determination at 3.2 A resolution, and analysis	Methanocaldococcus jannaschii

EC Number	Inhibitors	Comment	Organism	Structure
6.1.1.15	cysteinyl-sulfamoyl-adenylate	i.e. Cys-AMS, intermediate analogue, competitive inhibition	Methanothermobacter thermautotrophicus
6.1.1.15	prolyl-sulfamoyl-adenylate	i.e. Pro-AMS, intermediate analogue, competitive inhibition	Methanothermobacter thermautotrophicus

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism
6.1.1.15	0.05	-	L-cysteine	ATP-diphosphate exchange, pH 7.5, 35°C	Methanothermobacter thermautotrophicus
6.1.1.15	0.067	-	L-proline	ATP-diphosphate exchange reaction, pH 7.5, 35°C	Methanothermobacter thermautotrophicus
6.1.1.15	0.09	-	L-cysteine	ATP-diphosphate exchange reaction, pH 7.5, 35°C	Methanocaldococcus jannaschii
6.1.1.15	0.285	-	L-proline	ATP-diphosphate exchange reaction, pH 7.5, 35°C	Methanocaldococcus jannaschii

EC Number	Metals/Ions	Comment	Organism	Structure
6.1.1.15	Zn2+	chelated by 4 cysteine residues in the C-terminus	Methanothermobacter thermautotrophicus
6.1.1.15	Zn2+	chelated by 4 cysteine residues in the C-terminus	Methanocaldococcus jannaschii

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
6.1.1.15	ATP + L-proline + tRNAPro	Methanothermobacter thermautotrophicus	-	AMP + diphosphate + L-prolyl-tRNAPro	-	?
6.1.1.15	ATP + L-proline + tRNAPro	Methanocaldococcus jannaschii	-	AMP + diphosphate + L-prolyl-tRNAPro	-	?

EC Number	Organism	UniProt	Comment	Textmining
6.1.1.15	Methanocaldococcus jannaschii	-	-	-
6.1.1.15	Methanothermobacter thermautotrophicus	-	gene proS	-

EC Number	Purification (Comment)	Organism
6.1.1.15	recombinant His-tagged enzyme from Escherichia coli	Methanothermobacter thermautotrophicus
6.1.1.15	recombinant His-tagged enzyme from expression in Escherichia coli	Methanocaldococcus jannaschii

EC Number	Reaction	Comment	Organism	Reaction ID
6.1.1.15	ATP + L-proline + tRNAPro = AMP + diphosphate + L-prolyl-tRNAPro	dual substrate specificity	Methanothermobacter thermautotrophicus	a

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
6.1.1.15	ATP + L-cysteine + tRNACys	-	Methanothermobacter thermautotrophicus	AMP + diphosphate + L-cysteinyl-tRNACys	-	?
6.1.1.15	ATP + L-proline + tRNAPro	-	Methanothermobacter thermautotrophicus	AMP + diphosphate + L-prolyl-tRNAPro	-	?
6.1.1.15	ATP + L-proline + tRNAPro	-	Methanocaldococcus jannaschii	AMP + diphosphate + L-prolyl-tRNAPro	-	?
6.1.1.15	additional information	the enzyme also performs the ATP-diphosphate exchange reaction	Methanothermobacter thermautotrophicus	?	-	?

EC Number	Synonyms	Comment	Organism
6.1.1.15	Global RNA synthesis factor	-	Methanothermobacter thermautotrophicus
6.1.1.15	Global RNA synthesis factor	-	Methanocaldococcus jannaschii
6.1.1.15	Pro-tRNA synthetase	-	Methanothermobacter thermautotrophicus
6.1.1.15	Pro-tRNA synthetase	-	Methanocaldococcus jannaschii
6.1.1.15	Proline translase	-	Methanothermobacter thermautotrophicus
6.1.1.15	Proline translase	-	Methanocaldococcus jannaschii
6.1.1.15	Proline--tRNA ligase	-	Methanothermobacter thermautotrophicus
6.1.1.15	Proline--tRNA ligase	-	Methanocaldococcus jannaschii
6.1.1.15	Prolyl RNA synthetase	-	Methanothermobacter thermautotrophicus
6.1.1.15	Prolyl RNA synthetase	-	Methanocaldococcus jannaschii
6.1.1.15	Prolyl-transfer ribonucleate synthetase	-	Methanothermobacter thermautotrophicus
6.1.1.15	Prolyl-transfer ribonucleate synthetase	-	Methanocaldococcus jannaschii
6.1.1.15	Prolyl-transfer ribonucleic acid synthetase	-	Methanothermobacter thermautotrophicus
6.1.1.15	Prolyl-transfer ribonucleic acid synthetase	-	Methanocaldococcus jannaschii
6.1.1.15	Prolyl-transfer RNA synthetase	-	Methanothermobacter thermautotrophicus
6.1.1.15	Prolyl-transfer RNA synthetase	-	Methanocaldococcus jannaschii
6.1.1.15	Prolyl-tRNA synthetase	-	Methanothermobacter thermautotrophicus
6.1.1.15	Prolyl-tRNA synthetase	-	Methanocaldococcus jannaschii
6.1.1.15	ProRS	-	Methanothermobacter thermautotrophicus
6.1.1.15	ProRS	-	Methanocaldococcus jannaschii

EC Number	Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
6.1.1.15	35	-	assay at	Methanothermobacter thermautotrophicus
6.1.1.15	35	-	assay at	Methanocaldococcus jannaschii

EC Number	pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.1.1.15	7.5	-	assay at	Methanothermobacter thermautotrophicus
6.1.1.15	7.5	-	assay at	Methanocaldococcus jannaschii

EC Number	Cofactor	Comment	Organism	Structure
6.1.1.15	ATP	-	Methanothermobacter thermautotrophicus
6.1.1.15	ATP	-	Methanocaldococcus jannaschii

EC Number	Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
6.1.1.15	0.000025	-	cysteinyl-sulfamoyl-adenylate	versus L-proline, pH 7.5, 35°C	Methanothermobacter thermautotrophicus
6.1.1.15	0.00005	-	prolyl-sulfamoyl-adenylate	versus L-proline, pH 7.5, 35°C	Methanothermobacter thermautotrophicus