Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Munoz, A.; Piedras, P.; Aguilar, M.; Pineda, M.
    Urea is a product of ureidoglycolate degradation in chickpea. Purification and characterization of the ureidoglycolate urea-lyase (2001), Plant Physiol., 125, 828-834.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.3.2.3 EDTA complete inhibition, 50% of activity is restored after removal of EDTA, addition of Mn2+, Zn2+ or Mg2+ recover the activity to the initial levels Cicer arietinum
4.3.2.3 Fe2+ strong inactivation Cicer arietinum
4.3.2.3 Ni2+ strong inactivation Cicer arietinum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.3.2.3 0.006
-
 (-)-ureidoglycolate 30°C, pH 7.8, in the presence of 0.5 mM Mn2+ Cicer arietinum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.3.2.3 Mg2+ activation Cicer arietinum
4.3.2.3 Mn2+ 2fold increase in activity Cicer arietinum
4.3.2.3 Zn2+ activation Cicer arietinum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.3.2.3 31000
-
 6 * 31000, SDS-PAGE Cicer arietinum
4.3.2.3 180000
-
 gel filtration Cicer arietinum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.3.2.3 (S)-ureidoglycolate Cicer arietinum
-
 glyoxylate + urea
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.3.2.3 Cicer arietinum
-
 chickpea
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.3.2.3 ammonium sulfate, gel filtration, heat treatment, ion exchange, electrophoresis Cicer arietinum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.3.2.3 pod developing pods Cicer arietinum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.3.2.3 8.6
-
-
 Cicer arietinum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.2.3 (S)-ureidoglycolate
-
 Cicer arietinum glyoxylate + urea
-
 ?

Subunits

EC Number Subunits Comment Organism
4.3.2.3 hexamer 6 * 31000, SDS-PAGE Cicer arietinum

Synonyms

EC Number Synonyms Comment Organism
4.3.2.3 (-)-ureidoglycolate urea-lyase
-
 Cicer arietinum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.3.2.3 70
-
 and above, chemical instability of ureidoglycolate at higher temperatures Cicer arietinum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.3.2.3 30 70 approx. 30% of maximal activity at 40°C Cicer arietinum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.3.2.3 80
-
 no loss of activity after 60 min in the presence of Mn2+ Cicer arietinum
4.3.2.3 90
-
 complete loss of activity after 40 min in the presence of Mn2+ Cicer arietinum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.3.2.3 7 8
-
 Cicer arietinum