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Literature summary extracted from

  • Dharmawardhana, D.P.; Ellis, B.E.; Carlson, J.E.
    A beta-glucosidase from lodgepole pine xylem specific for the lignin precursor coniferin (1995), Plant Physiol., 107, 331-339.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.126 glucono-1,5-lactone
-
 Pinus contorta
3.2.1.126 additional information not inhibited by bromoconduritol or conduritol B epoxide Pinus contorta

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.126 0.18
-
 coniferin pH 5.5, 30°C Pinus contorta
3.2.1.126 0.29
-
 syringin pH 5.5, 30°C Pinus contorta
3.2.1.126 1.9
-
 4-nitrophenyl beta-D-glucoside pH 5.5, 30°C Pinus contorta
3.2.1.126 2.3
-
 4-methylumbelliferyl beta-D-glucoside pH 5.5, 30°C Pinus contorta

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.126 28000
-
 2 * 28000, SDS-PAGE Pinus contorta
3.2.1.126 60000
-
 gel filtration, native PAGE Pinus contorta

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.126 coniferin + H2O Pinus contorta native substrate, may play a crucial role in stem lignification D-glucose + coniferol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.126 Pinus contorta
-
 var. latifolia Engelm, 10-15 years old
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.126 glycoprotein
-
 Pinus contorta

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.126 2760fold Pinus contorta

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.126 xylem exclusive presence in the differentiating xylem Pinus contorta
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.126 6.6
-
 pH 5.5, 30°C Pinus contorta

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.126 2-nitrophenyl beta-D-glucoside + H2O 57% of the activity with coniferin Pinus contorta 2-nitrophenol + beta-D-glucose
-
 ?
3.2.1.126 4-methylumbelliferyl beta-D-glucoside + H2O 16% of the activity with coniferin Pinus contorta 4-methylumbelliferone + beta-D-glucose
-
 ?
3.2.1.126 4-nitrophenyl beta-D-glucoside + H2O 26% of the activity with coniferin Pinus contorta 4-nitrophenol + beta-D-glucose
-
 ?
3.2.1.126 5-[4-(beta-D-glucopyranosyloxy)-3-methoxyphenylmethylene]-2-thioxothiazolidin-4-one-3-ethanoic acid + H2O synthetic coniferin analog, 45% of the activity with coniferin Pinus contorta ?
-
 ?
3.2.1.126 coniferin + H2O the native substrate is efficiently hydrolyzed, preferred substrates Pinus contorta D-glucose + coniferol
-
 ?
3.2.1.126 coniferin + H2O native substrate, may play a crucial role in stem lignification Pinus contorta D-glucose + coniferol
-
 ?
3.2.1.126 additional information not: disaccharide-containing glycoside, 4-nitrophenyl beta-cellobioside, 4-methylumbelliferyl alpha-glucoside Pinus contorta ?
-
 ?
3.2.1.126 syringin + H2O 51% of the activity with coniferin Pinus contorta D-glucose + sinapyl alcohol
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.126 dimer 2 * 28000, SDS-PAGE Pinus contorta

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.126 30
-
 assay at Pinus contorta

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.126 5.4 5.9 optimum in the range at 30°C Pinus contorta

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.126 Pinus contorta chromatofocussing, pH gradient of 5.5 to 4
-
 4.5