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Literature summary extracted from

  • Nakano, K.; Suzuki, T.; Hayakawa, T.; Yamaya, T.
    Organ and cellular localization of asparagine synthetase in rice plants (2000), Plant Cell Physiol., 41, 874-880.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.5.4 a fusion protein consisting of the 42 kDa N-terminal region of AS and a 17 kDa tagged-region from pET32a(+) expression plasmid, expression in Escherichia coli Oryza sativa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.5.4 66000
-
 x * 66000, SDS-PAGE Oryza sativa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.5.4 additional information Oryza sativa nitrogen metabolism, asparagine synthesis ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.5.4 Oryza sativa
-
 cv. Sasanishiki
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.5.4 recombinant fusion protein consisting of the 42 kDa N-terminal region of AS and a 17 kDa tagged-region from pET32a(+) expression plasmid Oryza sativa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.3.5.4 caryopsis high content of AS in grains in the middle stage of ripening, in vascular tissues Oryza sativa
-
6.3.5.4 leaf high content of AS in leaf sheath at the second position from the fully expanded top leaf, the contents gradually decreases in leaf sheaths as a function of increasing age, in vascular tissues Oryza sativa
-
6.3.5.4 additional information organ and cellular localization Oryza sativa
-
6.3.5.4 spikelet during the ripening of the spikelets AS contents increases during the first 21 days after flowering, then declines rapidly Oryza sativa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.5.4 ATP + L-aspartate + L-glutamine
-
 Oryza sativa AMP + diphosphate + L-asparagine + L-glutamate
-
 ?
6.3.5.4 additional information nitrogen metabolism, asparagine synthesis Oryza sativa ?
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.5.4 ? x * 66000, SDS-PAGE Oryza sativa