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Literature summary extracted from
- Three Arabidopsis genes encoding proteins with differential activities for cysteine synthase and beta-cyanoalanine synthase (2000), Plant Cell Physiol., 41, 465-476.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.4.1.9 | expression in Escherichia coli | Arabidopsis thaliana |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.4.1.9 | 0.02 | - |
KCN | 30°C, pH 10.0 | Arabidopsis thaliana |  |
| 4.4.1.9 | 0.04 | - |
Na2S | 30°C, pH 8.0 | Arabidopsis thaliana | |
| 4.4.1.9 | 0.14 | - |
L-cysteine | 30°C, pH 10.0 | Arabidopsis thaliana | |
| 4.4.1.9 | 8.03 | - |
O-acetyl-L-serine | 30°C, pH 8.0 | Arabidopsis thaliana | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 4.4.1.9 | mitochondrion | - |
Arabidopsis thaliana | 5739 | - |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.4.1.9 | L-cysteine + HCN | Arabidopsis thaliana | - |
sulfide + L-3-cyanoalanine | - |
? | |
| 4.4.1.9 | O-acetyl-L-serine + sulfide | Arabidopsis thaliana | - |
L-cysteine + acetate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.4.1.9 | Arabidopsis thaliana | Q9S757 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.4.1.9 | recombinant AtcysC1 | Arabidopsis thaliana |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 4.4.1.9 | leaf | highest mRNA levels in mature rosette leaves | Arabidopsis thaliana | - |
| 4.4.1.9 | seedling | - |
Arabidopsis thaliana | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.4.1.9 | L-cysteine + HCN | - |
Arabidopsis thaliana | sulfide + L-3-cyanoalanine | - |
? | |
| 4.4.1.9 | L-cysteine + KCN | - |
Arabidopsis thaliana | L-3-cyanoalanin + K2S | - |
? | |
| 4.4.1.9 | L-cysteine + KCN | - |
Arabidopsis thaliana | L-3-cyanoalanine + K2S | - |
? | |
| 4.4.1.9 | L-cysteine + NaCN | - |
Arabidopsis thaliana | L-3-cyanoalanin + Na2S | - |
? | |
| 4.4.1.9 | O-acetyl-L-serine + sulfide | - |
Arabidopsis thaliana | L-cysteine + acetate | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 4.4.1.9 | AtcysC1 | - |
Arabidopsis thaliana |
| 4.4.1.9 | beta-cyanoalanine synthase | - |
Arabidopsis thaliana |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.4.1.9 | 1.5 | - |
Na2S | 30°C, pH 8.0 | Arabidopsis thaliana | |
| 4.4.1.9 | 2 | - |
O-acetyl-L-serine | 30°C, pH 8.0 | Arabidopsis thaliana | |
| 4.4.1.9 | 2.17 | - |
KCN | 30°C, pH 10.0 | Arabidopsis thaliana | |
| 4.4.1.9 | 2.67 | - |
L-cysteine | 30°C, pH 10.0 | Arabidopsis thaliana | |
| 4.4.1.9 | 2.94 | - |
KCN | 30°C, pH 10.0 | Arabidopsis thaliana | |
| 4.4.1.9 | 3 | 6 | L-cysteine | 30°C, pH 10.0 | Arabidopsis thaliana | |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.4.1.9 | pyridoxal 5'-phosphate | - |
Arabidopsis thaliana | |
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