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Literature summary extracted from

  • Reichert, A.; Baalmann, E.; Vetter, S.; Backhausen, J.E.; Scheibe, R.
    Activation properties of the redox-modulated chloroplast enzymes glyceraldehyde 3-phosphate dehydrogenase and fructose-1,6-bisphosphatase (2000), Physiol. Plant., 110, 330-341.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.3.11 Ca2+ in presence of fructose-1,6-bisphosphate, activates the native enzyme and recombinant FBPase4.5, not FBPase4.9 Spinacia oleracea
3.1.3.11 thioredoxin
-
Spinacia oleracea

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.11 expression in Escherichia coli Spinacia oleracea

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.11 chloroplast
-
Spinacia oleracea 9507
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.11 Mg2+ comparison of activity at 2, 10 and 15 mM Mg2+ of native and two recombinant enzymes Spinacia oleracea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.11 160000
-
gel filtration Spinacia oleracea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.11 D-fructose 1,6-bisphosphate + H2O Spinacia oleracea
-
D-fructose 6-phosphate + phosphate
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.11 Spinacia oleracea
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.11 recombinant enzyme Spinacia oleracea

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.11 additional information
-
-
Spinacia oleracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.11 D-fructose 1,6-bisphosphate + H2O
-
Spinacia oleracea D-fructose 6-phosphate + phosphate
-
?

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.3.11 additional information
-
comparison of activity at pH 8.0 and 8.8 of native and two recombinant enzymes Spinacia oleracea

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.3.11 Spinacia oleracea FBPase4.5, the recombinant enzyme in oxidized and reduced state has a pI value of 4.5, isoelectric focusing
-
4.5
3.1.3.11 Spinacia oleracea FBPase4.9, the recombinant enzyme in oxidized state has a pI value of 4.9, after reduction with DTT the pI value changes to 4.5 and remains at 4.5 even after re-oxidation, isoelectric focusing
-
4.9