EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.1.3.11 | Ca2+ | in presence of fructose-1,6-bisphosphate, activates the native enzyme and recombinant FBPase4.5, not FBPase4.9 | Spinacia oleracea | |
3.1.3.11 | thioredoxin | - |
Spinacia oleracea |
EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.3.11 | expression in Escherichia coli | Spinacia oleracea |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.1.3.11 | chloroplast | - |
Spinacia oleracea | 9507 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.1.3.11 | Mg2+ | comparison of activity at 2, 10 and 15 mM Mg2+ of native and two recombinant enzymes | Spinacia oleracea |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.3.11 | 160000 | - |
gel filtration | Spinacia oleracea |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.3.11 | D-fructose 1,6-bisphosphate + H2O | Spinacia oleracea | - |
D-fructose 6-phosphate + phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.3.11 | Spinacia oleracea | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.3.11 | recombinant enzyme | Spinacia oleracea |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.1.3.11 | additional information | - |
- |
Spinacia oleracea |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.3.11 | D-fructose 1,6-bisphosphate + H2O | - |
Spinacia oleracea | D-fructose 6-phosphate + phosphate | - |
? |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.1.3.11 | additional information | - |
comparison of activity at pH 8.0 and 8.8 of native and two recombinant enzymes | Spinacia oleracea |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
3.1.3.11 | Spinacia oleracea | FBPase4.5, the recombinant enzyme in oxidized and reduced state has a pI value of 4.5, isoelectric focusing | - |
4.5 |
3.1.3.11 | Spinacia oleracea | FBPase4.9, the recombinant enzyme in oxidized state has a pI value of 4.9, after reduction with DTT the pI value changes to 4.5 and remains at 4.5 even after re-oxidation, isoelectric focusing | - |
4.9 |