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Literature summary extracted from

  • Sriskanda, V.; Kelman, Z.; Hurwitz, J.; Shuman, S.
    Characterization of an ATP-dependent DNA ligase from the thermophilic archaeon Methanobacterium thermoautotrophicum (2000), Nucleic Acids Res., 28, 2221-2228.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.5.1.1 expression in Escherichia coli Methanothermobacter thermautotrophicus

Protein Variants

EC Number Protein Variants Comment Organism
6.5.1.1 D253A mutant DNA ligase reacts with ATP to form the covalent intermediate, but is unable to catalyze the full ligation reaction Methanothermobacter thermautotrophicus
6.5.1.1 K251A no DNA ligase-adenylate formation and no nick-joining Methanothermobacter thermautotrophicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.5.1.1 ammonium sulfate 10 mM, 60% inhibition Methanothermobacter thermautotrophicus
6.5.1.1 KCl 50 mM, 70% inhibition Methanothermobacter thermautotrophicus
6.5.1.1 NaCl 50 mM, 75% inhibition, 80 mM, 90% inhibition Methanothermobacter thermautotrophicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.5.1.1 0.0011
-
 ATP 60°C, pH 7.5 Methanothermobacter thermautotrophicus
6.5.1.1 0.0016
-
 dATP 60°C, pH 7.5 Methanothermobacter thermautotrophicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.5.1.1 Co2+ 10 mM, divalent cation required for activity Methanothermobacter thermautotrophicus
6.5.1.1 Mg2+ 10 mM, divalent cation required for activity Methanothermobacter thermautotrophicus
6.5.1.1 Mn2+ 10 mM, divalent cation required for activity Methanothermobacter thermautotrophicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.5.1.1 68000
-
 zonal velocity sedimentation Methanothermobacter thermautotrophicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.5.1.1 ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m Methanothermobacter thermautotrophicus
-
 AMP + diphosphate + (deoxyribonucleotide)m+n
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.5.1.1 Methanothermobacter thermautotrophicus Q50566
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.5.1.1 recombinant Mth ligase Methanothermobacter thermautotrophicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.5.1.1 ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m
-
 Methanothermobacter thermautotrophicus AMP + diphosphate + (deoxyribonucleotide)m+n
-
 ?
6.5.1.1 ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m strandjoining on a singly nicked DNA in the presence of a divalent cation and ATP Methanothermobacter thermautotrophicus AMP + diphosphate + (deoxyribonucleotide)m+n
-
 ?
6.5.1.1 dATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m strandjoining on a singly nicked DNA in the presence of a divalent cation and dATP, approx. 10% of activity with ATP Methanothermobacter thermautotrophicus dAMP + diphosphate + (deoxyribonucleotide)m+n
-
 ?

Subunits

EC Number Subunits Comment Organism
6.5.1.1 monomer 1 * 68000, SDS-PAGE Methanothermobacter thermautotrophicus

Synonyms

EC Number Synonyms Comment Organism
6.5.1.1 Mth ligase
-
 Methanothermobacter thermautotrophicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.5.1.1 60
-
-
 Methanothermobacter thermautotrophicus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
6.5.1.1 22 80 almost no activity at 22°C, approx. 25% of maximal activity at 40°C and 70°C Methanothermobacter thermautotrophicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5.1.1 7.5 8.8
-
 Methanothermobacter thermautotrophicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
6.5.1.1 6.5 9 approx. 20% of maximal activity at pH 6.5, approx. 90% of maximal activity at pH 9.0 Methanothermobacter thermautotrophicus