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Literature summary extracted from
- Purification and characterization of human DNA topoisomerase IIIalpha (1999), Nucleic Acids Res., 27, 2443-2450.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.6.2.1 | glycerol | high concentrations increase the efficiency of the DNA relaxation reaction | Homo sapiens |  |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 5.6.2.1 | Y337F | mutant enzyme is still able to bind single-stranded DNA but is unable to cleave the DNA substrate because of the absence of the active Tyr337 | Homo sapiens |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.6.2.1 | KCl | above 100 mM | Homo sapiens | |
| 5.6.2.1 | NaCl | above 100 mM | Homo sapiens | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.6.2.1 | Mg2+ | dependent on, low concentrations of MgCl2 are sufficient to obtain efficient catalysis | Homo sapiens | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.6.2.1 | Homo sapiens | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.6.2.1 | - |
Homo sapiens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.6.2.1 | negatively supercoiled DNA | the enzyme relaxes negatively supercoiled DNA in a distributive manner, leading to the total disappearance of the initial substrate and the appearance of intermediate topoisomers. The enzyme is able to cleave a single stranded oligonucleotide to bind covalently to the 5-end of cleaved DNA. The consensus sequence for DNA cleavage is CANNN-/- | Homo sapiens | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 5.6.2.1 | hTopo IIIalpha | - |
Homo sapiens |
| 5.6.2.1 | topoisomerase IIIalpha | - |
Homo sapiens |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.6.2.1 | 37 | 45 | - |
Homo sapiens |
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Release 2023.1 (January 2023)
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