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Literature summary extracted from

  • Nagy, L.; Hiripi, L.
    Role of tyrosine, DOPA and decarboxylase enzymes in the synthesis of monoamines in the brain of the locust (2002), Neurochem. Int., 41, 9-16.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.1.1.28 pyridoxal 5'-phosphate stimulates decarboxylation of DOPA Locusta migratoria

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.25 (2S)-2-amino-3-(3-hydroxyphenyl)-2-methylpropanoic acid IC50: 6.37 mM Locusta migratoria
4.1.1.25 (2S)-2-amino-3-(4-hydroxyphenyl)-2-methylpropanoic acid IC50: 9.4 mM Locusta migratoria
4.1.1.25 3-hydroxybenzyl-hydrazine potent inhibitor, IC50: 0.0004 mM Locusta migratoria
4.1.1.25 alpha-methyl-2,4-dihydroxyphenylalanine IC50: 1.8 mM Locusta migratoria
4.1.1.28 (2S)-2-amino-3-(3-hydroxyphenyl)-2-methylpropanoic acid
-
 Locusta migratoria
4.1.1.28 (2S)-2-amino-3-(4-hydroxyphenyl)-2-methylpropanoic acid
-
 Locusta migratoria
4.1.1.28 3-hydroxy-benzylhydrazine
-
 Locusta migratoria
4.1.1.28 3-iodo-L-tyrosine
-
 Locusta migratoria
4.1.1.28 alpha-methyl-2,4-dihydroxyphenylalanine
-
 Locusta migratoria

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.25 additional information
-
 additional information
-
 Locusta migratoria
4.1.1.25 0.007
-
 L-tyrosine pH 7, 32°C Locusta migratoria
4.1.1.25 0.117
-
 L-Dopa pH 7, 32°C Locusta migratoria
4.1.1.28 0.052
-
 3,4-dihydroxyphenylalanine pH 7.0, 32°C Locusta migratoria
4.1.1.28 0.084
-
 L-tyrosine pH 7.0, 32°C Locusta migratoria
4.1.1.28 0.23
-
 5-hydroxytryptophan pH 7.0, 32°C Locusta migratoria

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.25 370000
-
 gel filtration Locusta migratoria
4.1.1.28 112000
-
 gel filtration Locusta migratoria

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.25 L-Tyrosine Locusta migratoria
-
 Tyramine + CO2
-
 ?
4.1.1.28 3,4-dihydroxyphenylalanine Locusta migratoria regulation of the ganglionic dopamine level ? + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.25 Locusta migratoria
-
 migratorioides R.F., locust
-
4.1.1.28 Locusta migratoria
-
 migratory locust, subspecies migratorioides R.F.
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.25 partial Locusta migratoria
4.1.1.28 partial Locusta migratoria

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.1.1.25 brain cerebral ganglia Locusta migratoria
-
4.1.1.25 additional information enzyme activity decreases in the different ganglia in the following order: optic lobes, cerebral ganglia, thoracic ganglia, sub-oesophageal ganglion, abdominal ganglia Locusta migratoria
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.25 L-Dopa 16times lower affinity for DOPA than for tyrosine, the decarboxylation of DOPA is inhibited in the presence of the cofactor pyridoxal 5’-phosphate by 50-60%, maximum activity towards DOPA in the absence of the cofactor Locusta migratoria dopamine + CO2
-
 ?
4.1.1.25 L-Tyrosine
-
 Locusta migratoria Tyramine + CO2
-
 ?
4.1.1.25 L-Tyrosine 16times higher affinity for tyrosine than for DOPA Locusta migratoria Tyramine + CO2
-
 ?
4.1.1.25 additional information not: 5-hydroxytryptophan Locusta migratoria ?
-
 ?
4.1.1.28 3,4-dihydroxyphenylalanine
-
 Locusta migratoria 4-(2-aminoethyl)benzene-1,2-diol + CO2
-
 ?
4.1.1.28 3,4-dihydroxyphenylalanine regulation of the ganglionic dopamine level Locusta migratoria ? + CO2
-
 ?
4.1.1.28 5-hydroxytryptophan
-
 Locusta migratoria 5-hydroxytryptamine + CO2
-
 ?
4.1.1.28 L-tryptophan
-
 Locusta migratoria tryptamine + CO2
-
 ?
4.1.1.28 L-Tyrosine
-
 Locusta migratoria Tyramine + CO2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.1.1.25 TYR decarboxylase
-
 Locusta migratoria
4.1.1.28 5HTP decarboxylase
-
 Locusta migratoria
4.1.1.28 DOPA decarboxylase
-
 Locusta migratoria
4.1.1.28 DOPA/5HTP decarboxylase
-
 Locusta migratoria

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.1.25 32
-
 assay at Locusta migratoria

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.25 6.8 7.2
-
 Locusta migratoria
4.1.1.28 7
-
 5-hydroxytryptophan as substrate Locusta migratoria
4.1.1.28 7 7.4 2,4-dihydroxyphenylalanine as substrate Locusta migratoria
4.1.1.28 7.4 7.47 L-tyrosine as substrate Locusta migratoria

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.25 pyridoxal 5'-phosphate cofactor, presence inhibits decarboxylation of DOPA by 50-60% Locusta migratoria
4.1.1.28 pyridoxal 5'-phosphate
-
 Locusta migratoria

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
4.1.1.25 0.0004
-
 potent inhibitor, IC50: 0.0004 mM Locusta migratoria 3-hydroxybenzyl-hydrazine
4.1.1.25 1.8
-
 IC50: 1.8 mM Locusta migratoria alpha-methyl-2,4-dihydroxyphenylalanine
4.1.1.25 6.37
-
 IC50: 6.37 mM Locusta migratoria (2S)-2-amino-3-(3-hydroxyphenyl)-2-methylpropanoic acid
4.1.1.25 9.4
-
 IC50: 9.4 mM Locusta migratoria (2S)-2-amino-3-(4-hydroxyphenyl)-2-methylpropanoic acid