Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Stec, B.; Yang, H.; Johnson, K.A.; Chen, L.; Roberts, M.F.
    MJ0109 is an enzyme that is both an inositol monophosphatase and the 'missing' archaeal fructose-1,6-bisphosphatase (2000), Nat. Struct. Biol., 7, 1046-1050.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.25
-
 Archaeoglobus fulgidus
3.1.3.25
-
 Thermotoga maritima
3.1.3.25 expressed in Escherichia coli Methanocaldococcus jannaschii

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.3.25 hanging drop vapour diffusion method, best crystals in the presence of Zn2+ and phosphate Methanocaldococcus jannaschii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.25 0.038
-
 D-fructose 1,6-bisphosphate 85°C Methanocaldococcus jannaschii
3.1.3.25 0.07
-
 myo-inositol 1-phosphate 37°C Escherichia coli
3.1.3.25 0.091
-
 myo-inositol 1-phosphate 85°C Methanocaldococcus jannaschii
3.1.3.25 0.11
-
 myo-inositol 1-phosphate 85°C Archaeoglobus fulgidus
3.1.3.25 0.13
-
 myo-inositol 1-phosphate 95°C Thermotoga maritima

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.25 D-fructose 1,6-bisphosphate + H2O Archaeoglobus fulgidus very effective substrate D-fructose 6-phosphate + phosphate
-
 ?
3.1.3.25 D-fructose 1,6-bisphosphate + H2O Thermotoga maritima very effective substrate D-fructose 6-phosphate + phosphate
-
 ?
3.1.3.25 D-fructose 1,6-bisphosphate + H2O Methanocaldococcus jannaschii very effective substrate D-fructose 6-phosphate + phosphate
-
 ?
3.1.3.25 D-fructose 1,6-bisphosphate + H2O Thermotoga maritima TM1415 very effective substrate D-fructose 6-phosphate + phosphate
-
 ?
3.1.3.25 D-fructose 1,6-bisphosphate + H2O Archaeoglobus fulgidus AF2372 very effective substrate D-fructose 6-phosphate + phosphate
-
 ?
3.1.3.25 myo-inositol 1-phosphate + H2O Escherichia coli
-
 myo-inositol + phosphate
-
 ?
3.1.3.25 myo-inositol 1-phosphate + H2O Archaeoglobus fulgidus organism do not contain lipids with inositol head group, involved in the response to thermal stress myo-inositol + phosphate
-
 ?
3.1.3.25 myo-inositol 1-phosphate + H2O Thermotoga maritima organism do not contain lipids with inositol head group, involved in the response to thermal stress myo-inositol + phosphate
-
 ?
3.1.3.25 myo-inositol 1-phosphate + H2O Methanocaldococcus jannaschii organism do not contain lipids with inositol head group, involved in the response to thermal stress myo-inositol + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.25 Archaeoglobus fulgidus
-
-
-
3.1.3.25 Archaeoglobus fulgidus AF2372
-
-
-
3.1.3.25 Escherichia coli
-
-
-
3.1.3.25 Methanocaldococcus jannaschii Q57573
-
-
3.1.3.25 Thermotoga maritima
-
-
-
3.1.3.25 Thermotoga maritima TM1415
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.25
-
 Archaeoglobus fulgidus
3.1.3.25
-
 Thermotoga maritima
3.1.3.25
-
 Methanocaldococcus jannaschii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.25 2'-AMP + H2O 600% of the rate with inositol 1-phosphate Methanocaldococcus jannaschii adenosine + phosphate
-
 ?
3.1.3.25 4-nitrophenyl phosphate + H2O very effective substrate Archaeoglobus fulgidus 4-nitrophenol + phosphate
-
 ?
3.1.3.25 4-nitrophenyl phosphate + H2O very effective substrate Thermotoga maritima 4-nitrophenol + phosphate
-
 ?
3.1.3.25 4-nitrophenyl phosphate + H2O very effective substrate Methanocaldococcus jannaschii 4-nitrophenol + phosphate
-
 ?
3.1.3.25 4-nitrophenyl phosphate + H2O very effective substrate Thermotoga maritima TM1415 4-nitrophenol + phosphate
-
 ?
3.1.3.25 4-nitrophenyl phosphate + H2O very effective substrate Archaeoglobus fulgidus AF2372 4-nitrophenol + phosphate
-
 ?
3.1.3.25 alpha-D-glucose 1-phosphate + H2O
-
 Archaeoglobus fulgidus alpha-D-glucose + phosphate
-
 ?
3.1.3.25 alpha-D-glucose 1-phosphate + H2O
-
 Thermotoga maritima alpha-D-glucose + phosphate
-
 ?
3.1.3.25 alpha-D-glucose 1-phosphate + H2O
-
 Methanocaldococcus jannaschii alpha-D-glucose + phosphate
-
 ?
3.1.3.25 alpha-D-glucose 1-phosphate + H2O
-
 Thermotoga maritima TM1415 alpha-D-glucose + phosphate
-
 ?
3.1.3.25 alpha-D-glucose 1-phosphate + H2O
-
 Archaeoglobus fulgidus AF2372 alpha-D-glucose + phosphate
-
 ?
3.1.3.25 D-fructose 1,6-bisphosphate + H2O very effective substrate Archaeoglobus fulgidus D-fructose 6-phosphate + phosphate
-
 ?
3.1.3.25 D-fructose 1,6-bisphosphate + H2O very effective substrate Thermotoga maritima D-fructose 6-phosphate + phosphate
-
 ?
3.1.3.25 D-fructose 1,6-bisphosphate + H2O very effective substrate Methanocaldococcus jannaschii D-fructose 6-phosphate + phosphate
-
 ?
3.1.3.25 D-fructose 1,6-bisphosphate + H2O very effective substrate Thermotoga maritima TM1415 D-fructose 6-phosphate + phosphate
-
 ?
3.1.3.25 D-fructose 1,6-bisphosphate + H2O very effective substrate Archaeoglobus fulgidus AF2372 D-fructose 6-phosphate + phosphate
-
 ?
3.1.3.25 D-fructose 2,6-bisphosphate + H2O 16% of the rate with inositol 1-phosphate Methanocaldococcus jannaschii D-fructose 6-phosphate + phosphate
-
 ?
3.1.3.25 glycerol 2-phosphate + H2O
-
 Methanocaldococcus jannaschii glycerol + phosphate
-
 ?
3.1.3.25 additional information archeal enzyme displays inositol monophosphatase as well as fructose 1,6-bisphosphatase activity Archaeoglobus fulgidus ?
-
 ?
3.1.3.25 additional information archeal enzyme displays inositol monophosphatase as well as fructose 1,6-bisphosphatase activity Thermotoga maritima ?
-
 ?
3.1.3.25 additional information D-glucose 6-phosphate, D-fructose 6-phosphate, 5'-AMP and NAD+ are not hydrolyzed Methanocaldococcus jannaschii ?
-
 ?
3.1.3.25 additional information traces of activity with glucose 1-phosphate, no activity with 4-nitrophenyl phosphate and fructose 1,6-bisphosphate Escherichia coli ?
-
 ?
3.1.3.25 additional information archeal enzyme displays inositol monophosphatase as well as fructose 1,6-bisphosphatase activity Thermotoga maritima TM1415 ?
-
 ?
3.1.3.25 additional information archeal enzyme displays inositol monophosphatase as well as fructose 1,6-bisphosphatase activity Archaeoglobus fulgidus AF2372 ?
-
 ?
3.1.3.25 myo-inositol 1-phosphate + H2O
-
 Escherichia coli myo-inositol + phosphate
-
 ?
3.1.3.25 myo-inositol 1-phosphate + H2O
-
 Archaeoglobus fulgidus myo-inositol + phosphate
-
 ?
3.1.3.25 myo-inositol 1-phosphate + H2O
-
 Thermotoga maritima myo-inositol + phosphate
-
 ?
3.1.3.25 myo-inositol 1-phosphate + H2O
-
 Methanocaldococcus jannaschii myo-inositol + phosphate
-
 ?
3.1.3.25 myo-inositol 1-phosphate + H2O organism do not contain lipids with inositol head group, involved in the response to thermal stress Archaeoglobus fulgidus myo-inositol + phosphate
-
 ?
3.1.3.25 myo-inositol 1-phosphate + H2O organism do not contain lipids with inositol head group, involved in the response to thermal stress Thermotoga maritima myo-inositol + phosphate
-
 ?
3.1.3.25 myo-inositol 1-phosphate + H2O organism do not contain lipids with inositol head group, involved in the response to thermal stress Methanocaldococcus jannaschii myo-inositol + phosphate
-
 ?
3.1.3.25 myo-inositol 1-phosphate + H2O
-
 Thermotoga maritima TM1415 myo-inositol + phosphate
-
 ?
3.1.3.25 myo-inositol 1-phosphate + H2O
-
 Archaeoglobus fulgidus AF2372 myo-inositol + phosphate
-
 ?
3.1.3.25 myo-inositol 2-phosphate + H2O 15% of the rate with inositol 1-phosphate Methanocaldococcus jannaschii myo-inositol + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.25 dimer
-
 Archaeoglobus fulgidus
3.1.3.25 dimer crystal structure analysis Methanocaldococcus jannaschii
3.1.3.25 monomer
-
 Escherichia coli
3.1.3.25 tetramer
-
 Thermotoga maritima

Synonyms

EC Number Synonyms Comment Organism
3.1.3.25 More archeal enzyme displays inositol monophosphatase as well as fructose 1,6-bisphosphatase activity Methanocaldococcus jannaschii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.3.25 1.5
-
 myo-inositol 1-phosphate 50°C Methanocaldococcus jannaschii
3.1.3.25 2.5
-
 myo-inositol 1-phosphate 85°C Archaeoglobus fulgidus
3.1.3.25 2.7
-
 D-fructose 1,6-bisphosphate 50°C Methanocaldococcus jannaschii
3.1.3.25 2.7
-
 D-fructose 1,6-bisphosphate 85°C Archaeoglobus fulgidus
3.1.3.25 4.2
-
 myo-inositol 1-phosphate 85°C Methanocaldococcus jannaschii
3.1.3.25 6.5
-
 myo-inositol 1-phosphate 37°C Escherichia coli
3.1.3.25 7
-
 D-fructose 1,6-bisphosphate 85°C Methanocaldococcus jannaschii
3.1.3.25 207
-
 myo-inositol 1-phosphate 95°C Thermotoga maritima
3.1.3.25 268
-
 D-fructose 1,6-bisphosphate 95°C Thermotoga maritima