BRENDA - Enzyme Database show

Mechanism of metal activation of human hematopoietic prostaglandin D synthase

Inoue, T.; Irikura, D.; Okazaki, N.; Kinugasa, S.; Matsumura, H.; Uodome, N.; Yamamoto, M.; Kumasaka, T.; Miyano, M.; Kai, Y.; Urade, Y.; Nat. Struct. Biol. 10, 291-296 (2003)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
5.3.99.2
-
Homo sapiens
Crystallization (Commentary)
EC Number
Crystallization
Organism
5.3.99.2
macro-seeding using a solution containing 14% polyethylene glycol 6000, 50 mM Tris-HCl, pH 8.4, 5 mM reduced glutathione, 5 mM dithiothreitol, 2.5 mM CaCl2 or MgCl2 and 1% dioxane, structure resolution at 1.8 A
Homo sapiens
Engineering
EC Number
Amino acid exchange
Commentary
Organism
5.3.99.2
D93N
not activated by Ca2+ or Mg2+
Homo sapiens
5.3.99.2
D96N
very high basal activity, no activation in the presence of Ca2+ or Mg2+
Homo sapiens
5.3.99.2
D97N
not activated by Ca2+ or Mg2+
Homo sapiens
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
5.3.99.2
Ba2+
increase in activity
Homo sapiens
5.3.99.2
Ca2+
half maximal effective concentration at 0.4 mM, 1.5fold increase in activity
Homo sapiens
5.3.99.2
Mg2+
half maximal effective concentration at 0.05 mM, 1.5fold increase in activity, Mg2+ increases the affinity for glutathione
Homo sapiens
5.3.99.2
Sr2+
increase in activity
Homo sapiens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5.3.99.2
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate + glutathione
Homo sapiens
-
(5Z,13E)-(15S)-9alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate + glutathione
-
Homo sapiens
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
5.3.99.2
Homo sapiens
O60760
-
-
Purification (Commentary)
EC Number
Commentary
Organism
5.3.99.2
recombinant PDGS
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.3.99.2
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate + glutathione
-
653299
Homo sapiens
(5Z,13E)-(15S)-9alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate + glutathione
-
653299
Homo sapiens
?
Subunits
EC Number
Subunits
Commentary
Organism
5.3.99.2
dimer
crystallization
Homo sapiens
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
5.3.99.2
glutathione
-
Homo sapiens
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
5.3.99.2
-
Homo sapiens
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
5.3.99.2
glutathione
-
Homo sapiens
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
5.3.99.2
macro-seeding using a solution containing 14% polyethylene glycol 6000, 50 mM Tris-HCl, pH 8.4, 5 mM reduced glutathione, 5 mM dithiothreitol, 2.5 mM CaCl2 or MgCl2 and 1% dioxane, structure resolution at 1.8 A
Homo sapiens
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
5.3.99.2
D93N
not activated by Ca2+ or Mg2+
Homo sapiens
5.3.99.2
D96N
very high basal activity, no activation in the presence of Ca2+ or Mg2+
Homo sapiens
5.3.99.2
D97N
not activated by Ca2+ or Mg2+
Homo sapiens
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
5.3.99.2
Ba2+
increase in activity
Homo sapiens
5.3.99.2
Ca2+
half maximal effective concentration at 0.4 mM, 1.5fold increase in activity
Homo sapiens
5.3.99.2
Mg2+
half maximal effective concentration at 0.05 mM, 1.5fold increase in activity, Mg2+ increases the affinity for glutathione
Homo sapiens
5.3.99.2
Sr2+
increase in activity
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5.3.99.2
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate + glutathione
Homo sapiens
-
(5Z,13E)-(15S)-9alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate + glutathione
-
Homo sapiens
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
5.3.99.2
recombinant PDGS
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.3.99.2
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate + glutathione
-
653299
Homo sapiens
(5Z,13E)-(15S)-9alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate + glutathione
-
653299
Homo sapiens
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
5.3.99.2
dimer
crystallization
Homo sapiens