Literature summary extracted from

Peters, J.E.; Park, S.J.; Darzins, A.; Freck, L.C.; Saulnier, J.M.; Wallach, J.M.; Galloway, D.R.
Further studies on Pseudomonas aeruginosa LasA: analysis of specificity (1992), Mol. Microbiol., 6, 1155-1162.

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
3.4.24.B16	additional information	no inhibition by EDTA and o-phenanthroline	Pseudomonas aeruginosa
3.4.24.B16	N-tosyl-L-lysine chloromethyl ketone	-	Pseudomonas aeruginosa
3.4.24.B16	Phenylmethylsulfonylfluoride	-	Pseudomonas aeruginosa

Localization

EC Number	Localization	Comment	Organism	GeneOntology No.	Textmining
3.4.24.B16	extracellular	active fragment	Pseudomonas aeruginosa	-	-

Metals/Ions

EC Number	Metals/Ions	Comment	Organism	Structure
3.4.24.B16	additional information	enzyme is no metalloprotease	Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
3.4.24.B16	protein + H2O	Pseudomonas aeruginosa	-	peptides	-	?

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.4.24.B16	Pseudomonas aeruginosa	-	-	-

Reaction

EC Number	Reaction	Comment	Organism	Reaction ID
3.4.24.B16	proteolytic degradation of proteins	enzyme might be a modified serine protease with a His residue in the active site, specifically involved in degradation of elastin	Pseudomonas aeruginosa	a

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3.4.24.B16	beta-casein + H2O	only 1 cleavage site: in the sequence NKKIEKFQphosphorylated-S between Lys and Ile, serine protease activity	Pseudomonas aeruginosa	beta-casein + 30 amino acid residues fragment	-	?
3.4.24.B16	Elastin + H2O	cleavage of elastin by the enzyme possibly results in the unfolding or disruption of the complex elastin structure, thus providing more access to elastase and other proteases, elastolytic activity in absence of elastase, enzyme also enhances the elastolytic activity of elastase together with alkaline phophatase	Pseudomonas aeruginosa	?	-	?
3.4.24.B16	additional information	no activity with tosyl-Gly-Pro-Lys-4-nitroanilide, enzyme activates elastase, and the elastolytic, not the proteolytic, activity of thermolysin, human neutrophil elastase, proteinase K, by interacting with the elastin substrate rather than the other enzymes	Pseudomonas aeruginosa	?	-	?
3.4.24.B16	protein + H2O	-	Pseudomonas aeruginosa	peptides	-	?

Synonyms

EC Number	Synonyms	Comment	Organism
3.4.24.B16	LasA	-	Pseudomonas aeruginosa
3.4.24.B16	M23.002	Merops-ID	Pseudomonas aeruginosa
3.4.24.B16	staphylolysin	-	Pseudomonas aeruginosa

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Release 2023.1 (January 2023)