BRENDA - Enzyme Database

Characterization of a non-mitochondrial type I phosphatidylserine decarboxylase in Plasmodium falciparum

Baunaure, F.; Eldin, P.; Cathiard, A.M.; Vial, H.; Mol. Microbiol. 51, 33-46 (2004)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
4.1.1.65
Triton X-100
enzyme activity depends on presence of Triton X-100, optimum concentration 0.052%
Plasmodium falciparum
Cloned(Commentary)
EC Number
Commentary
Organism
4.1.1.65
-
Plasmodium falciparum
Engineering
EC Number
Amino acid exchange
Commentary
Organism
4.1.1.65
G315A
no processing of pro-enzyme, pro-enzyme is inactive
Plasmodium falciparum
4.1.1.65
G315A/S316A
no processing of pro-enzyme, pro-enzyme is inactive
Plasmodium falciparum
4.1.1.65
S316A
no processing of pro-enzyme
Plasmodium falciparum
4.1.1.65
S316T
no processing of pro-enzyme
Plasmodium falciparum
4.1.1.65
V314L/S317T
processing occurs to a slightly greater extent as in wild type, enzyme is twice as active
Plasmodium falciparum
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.1.65
63
-
phosphatidyl-L-serine
pH 6.8, recombinant enzyme
Plasmodium falciparum
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
4.1.1.65
membrane
-
Plasmodium falciparum
16020
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.1.1.65
35000
-
x * 42600, deduced from gene sequence, x * 35000, SDS-PAGE
Plasmodium falciparum
4.1.1.65
42600
-
x * 42600, deduced from gene sequence, x * 35000, SDS-PAGE
Plasmodium falciparum
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.1.1.65
Plasmodium falciparum
Q9GPP8
parasite-encoded enzyme in human erythrocytes
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.1.65
Phosphatidyl-L-serine
-
653276
Plasmodium falciparum
Phosphatidylethanolamine + CO2
-
653276
Plasmodium falciparum
?
Subunits
EC Number
Subunits
Commentary
Organism
4.1.1.65
?
x * 42600, deduced from gene sequence, x * 35000, SDS-PAGE
Plasmodium falciparum
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.1.1.65
6.8
-
strictly pH-dependent
Plasmodium falciparum
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
4.1.1.65
Triton X-100
enzyme activity depends on presence of Triton X-100, optimum concentration 0.052%
Plasmodium falciparum
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
4.1.1.65
-
Plasmodium falciparum
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
4.1.1.65
G315A
no processing of pro-enzyme, pro-enzyme is inactive
Plasmodium falciparum
4.1.1.65
G315A/S316A
no processing of pro-enzyme, pro-enzyme is inactive
Plasmodium falciparum
4.1.1.65
S316A
no processing of pro-enzyme
Plasmodium falciparum
4.1.1.65
S316T
no processing of pro-enzyme
Plasmodium falciparum
4.1.1.65
V314L/S317T
processing occurs to a slightly greater extent as in wild type, enzyme is twice as active
Plasmodium falciparum
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.1.65
63
-
phosphatidyl-L-serine
pH 6.8, recombinant enzyme
Plasmodium falciparum
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
4.1.1.65
membrane
-
Plasmodium falciparum
16020
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.1.1.65
35000
-
x * 42600, deduced from gene sequence, x * 35000, SDS-PAGE
Plasmodium falciparum
4.1.1.65
42600
-
x * 42600, deduced from gene sequence, x * 35000, SDS-PAGE
Plasmodium falciparum
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.1.65
Phosphatidyl-L-serine
-
653276
Plasmodium falciparum
Phosphatidylethanolamine + CO2
-
653276
Plasmodium falciparum
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.1.1.65
?
x * 42600, deduced from gene sequence, x * 35000, SDS-PAGE
Plasmodium falciparum
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.1.1.65
6.8
-
strictly pH-dependent
Plasmodium falciparum