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Literature summary extracted from

  • Cha, J.; Batt, C.A.
    Lowering the pH optimum of D-xylose isomerase: the effect of mutations of the negatively charged residues (1998), Mol. Cells, 8, 374-382.
    View publication on PubMed

Application

EC Number Application Comment Organism
5.3.1.5 synthesis production of high fructose corn syrup Streptomyces rubiginosus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.1.5 expression in Escherichia coli Streptomyces rubiginosus

Protein Variants

EC Number Protein Variants Comment Organism
5.3.1.5 D163N/E167Q 40-60% of wild-type activity, lower pH optimum than wild-type, nearly same tehrmostability as wild-type Streptomyces rubiginosus
5.3.1.5 D56N turnover number increased by 30-40% over that ofwild-type at pH 7.3, lower pH optimum than wild-type, nearly same thermostability as wild-type Streptomyces rubiginosus
5.3.1.5 D65A 40-60% of wild-type activity, lower pH optimum than wild-type, nearly same tehrmostability as wild-type Streptomyces rubiginosus
5.3.1.5 D81A 40-60% of wild-type activity, lower pH optimum than wild-type, nearly same tehrmostability as wild-type Streptomyces rubiginosus
5.3.1.5 E221A turnover number increased by 30-40% over that from wild-type at pH 7.3, lower pH optimum than wild-type, nearly same tehrmostability as wild-type Streptomyces rubiginosus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.1.5 160
-
 D-glucose 60°C, pH 7.3, with Co2+, wild-type Streptomyces rubiginosus
5.3.1.5 180
-
 D-glucose 60°C, pH 7.3, with Mg2+ and Co2+, mutant D81A and wild-type Streptomyces rubiginosus
5.3.1.5 190
-
 D-glucose 60°C, pH 7.3, with Mg2+ and Co2+, mutant D65A Streptomyces rubiginosus
5.3.1.5 200
-
 D-glucose 60°C, pH 7.3, with Mg2+ and Co2+, mutant D163N/E167Q Streptomyces rubiginosus
5.3.1.5 310
-
 D-glucose 60°C, pH 7.3, with Mg2+ and Co2+, mutants E221A and D56N Streptomyces rubiginosus
5.3.1.5 340
-
 D-glucose 60°C, pH 7.3, with Co2+, mutant E221A Streptomyces rubiginosus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.3.1.5 Co2+
-
 Streptomyces rubiginosus
5.3.1.5 Mg2+
-
 Streptomyces rubiginosus
5.3.1.5 Mn2+
-
 Streptomyces rubiginosus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.3.1.5 D-Xylose Streptomyces rubiginosus part of xylose metabolism D-Xylulose
-
 r

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.5 Streptomyces rubiginosus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.5 D-Glucose
-
 Streptomyces rubiginosus D-Fructose
-
 r
5.3.1.5 D-Xylose
-
 Streptomyces rubiginosus D-Xylulose
-
 r
5.3.1.5 D-Xylose part of xylose metabolism Streptomyces rubiginosus D-Xylulose
-
 r

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.3.1.5 0.03 0.55 D-glucose 60°C, pH 7.3, with Mg2+ and Co2+, mutant E221A Streptomyces rubiginosus
5.3.1.5 0.031 0.51 D-glucose 60°C, pH 7.3, with Mg2+ and Co2+, mutant D56N Streptomyces rubiginosus
5.3.1.5 0.04 1.97 D-glucose 60°C, pH 7.3, with Co2+, mutant E221A Streptomyces rubiginosus
5.3.1.5 3 6 D-glucose 60°C, pH 7.3, with Mg2+ and Co2+, mutant D163N/E167Q Streptomyces rubiginosus
5.3.1.5 3 6 D-glucose 60°C, pH 7.3, with Mg2+ and Co2+, mutant D65A Streptomyces rubiginosus
5.3.1.5 3 6 D-glucose 60°C, pH 7.3, with Mg2+ and Co2+, mutant D81A Streptomyces rubiginosus
5.3.1.5 3.02
-
 D-glucose 60°C, pH 7.3, with Mg2+ and Co2+, mutant D163N/E167Q Streptomyces rubiginosus
5.3.1.5 3.3
-
 D-glucose 60°C, pH 7.3, with Co2+, wild-type Streptomyces rubiginosus
5.3.1.5 3.3
-
 D-glucose 60°C, pH 7.3, with Mg2+ and Co2+, wild-type Streptomyces rubiginosus
5.3.1.5 3.37
-
 D-glucose 60°C, pH 7.3, with Mg2+ and Co2+, mutant D65A Streptomyces rubiginosus
5.3.1.5 3.41
-
 D-glucose 60°C, pH 7.3, with Mg2+ and Co2+, mutant D81A Streptomyces rubiginosus
5.3.1.5 4.53
-
 D-glucose 60°C, pH 7.3, with Co2+, wild-type Streptomyces rubiginosus
5.3.1.5 5.27
-
 D-glucose 60°C, pH 7.3, with Mg2+ and Co2+, wild-type Streptomyces rubiginosus
5.3.1.5 6.99
-
 D-glucose 60°C, pH 7.3, with Mg2+ and Co2+, mutant E221A Streptomyces rubiginosus
5.3.1.5 7.53
-
 D-glucose 60°C, pH 7.3, with Mg2+ and Co2+, mutant D56N Streptomyces rubiginosus
5.3.1.5 9.22
-
 D-glucose 60°C, pH 7.3, with Co2+, mutant E221A Streptomyces rubiginosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3.1.5 8
-
 in presence of Co2+, mutant E221A Streptomyces rubiginosus
5.3.1.5 8
-
 in presence of Mg2+ and Co2+, mutants E221A and D56N Streptomyces rubiginosus
5.3.1.5 8 8.2 in presence of Mg2+ and Co2+, mutants D65A and D163N/E167Q Streptomyces rubiginosus
5.3.1.5 8.1 8.3 in presence of Mg2+ and Co2+, mutant D81A Streptomyces rubiginosus
5.3.1.5 8.5
-
 in presence of Mg2+ and Co2+, wild-type Streptomyces rubiginosus
5.3.1.5 8.8
-
 in presence of Co2+, wild-type Streptomyces rubiginosus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
5.3.1.5 7.5 9.5
-
 Streptomyces rubiginosus